ZKSCAN3 Antibody - #DF7752

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製品: ZKSCAN3 Antibody
カタログ: DF7752
タンパク質の説明: Rabbit polyclonal antibody to ZKSCAN3
アプリケーション: WB IF/ICC
反応性: Human, Mouse
分子量: 61 kDa; 61kD(Calculated).
ユニプロット: Q9BRR0
RRID: AB_2841218

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MSDS
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IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Polyclonal
特異性:
ZKSCAN3 Antibody detects endogenous levels of total ZKSCAN3.
RRID:
AB_2841218
引用形式: Affinity Biosciences Cat# DF7752, RRID:AB_2841218.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

dJ874C20.1; mszf35; SCAN-KRAB-zinc finger protein; Skz1; Zf47; Zfp-47; ZFP306; Zfp307; Zfp47; Zinc finger and SCAN domain containing protein 13; Zinc finger and SCAN domain-containing protein 13; Zinc finger protein 306; Zinc finger protein 306, isoform CRA_a; Zinc finger protein 307; Zinc finger protein 309; Zinc finger protein 47 homolog; Zinc finger protein with KRAB and SCAN domains 3; Zinc finger protein with KRAB and SCAN domains 3; Zinc finger protein zfp47; Zinc finger with KRAB and SCAN domains 3; ZKSC3_HUMAN; ZKSCAN3; ZNF309; ZSCAN13; ZSCAN35;

免疫原

免疫原:
Uniprot:

Q9BRR0(ZKSC3_HUMAN) >>Visit HPA database.

遺伝子(ID):
ZKSCAN3(80317)
タンパク質配列:
MARELSESTALDAQSTEDQMELLVIKVEEEEAGFPSSPDLGSEGSRERFRGFRYPEAAGPREALSRLRELCRQWLQPEMHSKEQILELLVLEQFLTILPGNLQSWVREQHPESGEEVVVLLEYLERQLDEPAPQVSGVDQGQELLCCKMALLTPAPGSQSSQFQLMKALLKHESVGSQPLQDRVLQVPVLAHGGCCREDKVVASRLTPESQGLLKVEDVALTLTPEWTQQDSSQGNLCRDEKQENHGSLVSLGDEKQTKSRDLPPAEELPEKEHGKISCHLREDIAQIPTCAEAGEQEGRLQRKQKNATGGRRHICHECGKSFAQSSGLSKHRRIHTGEKPYECEECGKAFIGSSALVIHQRVHTGEKPYECEECGKAFSHSSDLIKHQRTHTGEKPYECDDCGKTFSQSCSLLEHHRIHTGEKPYQCSMCGKAFRRSSHLLRHQRIHTGDKNVQEPEQGEAWKSRMESQLENVETPMSYKCNECERSFTQNTGLIEHQKIHTGEKPYQCNACGKGFTRISYLVQHQRSHVGKNILSQ

PTMs - Q9BRR0 基板として

Site PTM Type Enzyme
Y54 Phosphorylation
S65 Phosphorylation
K82 Methylation
K171 Ubiquitination
T207 Phosphorylation
T222 Phosphorylation
T224 Phosphorylation
T228 Phosphorylation
T337 Phosphorylation
T365 Phosphorylation
T391 Phosphorylation
T393 Phosphorylation
T421 Phosphorylation
T449 Phosphorylation
Y480 Phosphorylation
T503 Phosphorylation
K515 Acetylation

研究背景

機能:

Transcriptional factor that binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3' and acts as a repressor of autophagy. Specifically represses expression of genes involved in autophagy and lysosome biogenesis/function such as MAP1LC3B, ULK1 or WIPI2. Associates with chromatin at the ITGB4 and VEGF promoters. Also acts as a transcription activator and promotes cancer cell progression and/or migration in various tumors and myelomas.

細胞の位置付け:

Nucleus. Cytoplasm.
Note: Mainly localizes in the nucleus. Under starvation conditions translocates to the cytoplasm, allowing expression of target genes involved in autophagy and lysosome biogenesis/function.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the krueppel C2H2-type zinc-finger protein family.

参考文献

1). Effect of salt promote the muscle triglyceride hydrolysis during dry-salting by inducing the phosphorylation of adipose tissue triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) and lipid droplets splitting. Food Chemistry, 2020 (PubMed: 32454271) [IF=8.8]

Application: WB    Species: Pig    Sample: adipose

Fig. 3. Western blotting analysis of ATGL, HSL and their phosphorylated protein contents in adipose tissue during processing of dry-cured meat. (A) WB image of each dry-cured group. (B) ATGL relative protein level in adipose tissue. (C) p-ATGL(S406) relative protein level in adipose tissue. (D) HSL relative protein level in adipose tissue. (E) p-HSL(Ser563) relative protein level in adipose tissue. (F) p-HSL(Ser660) relative protein level in adipose tissue.
2). Targeting ONECUT2 inhibits tumor angiogenesis via down-regulating ZKSCAN3/VEGFA. Biochemical pharmacology, 2024 (PubMed: 38797268) [IF=5.8]

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