NODAL Antibody - #DF7791

(1)   (2)  
製品: NODAL Antibody
カタログ: DF7791
タンパク質の説明: Rabbit polyclonal antibody to NODAL
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 40 kDa, 30 kDa; 40kD(Calculated).
ユニプロット: Q96S42
RRID: AB_2841248

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
NODAL Antibody detects endogenous levels of total NODAL.
RRID:
AB_2841248
引用形式: Affinity Biosciences Cat# DF7791, RRID:AB_2841248.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

HTX5; MGC138230; Nodal; Nodal Growth Differentiation Factor; nodal homolog (mouse); Nodal homolog; Nodal, Mouse, Homolog; NODAL_HUMAN;

免疫原

免疫原:
Uniprot:

Q96S42(NODAL_HUMAN) >>Visit HPA database.

遺伝子(ID):
NODAL(4838)
タンパク質配列:
MHAHCLPFLLHAWWALLQAGAATVATALLRTRGQPSSPSPLAYMLSLYRDPLPRADIIRSLQAEDVAVDGQNWTFAFDFSFLSQQEDLAWAELRLQLSSPVDLPTEGSLAIEIFHQPKPDTEQASDSCLERFQMDLFTVTLSQVTFSLGSMVLEVTRPLSKWLKHPGALEKQMSRVAGECWPRPPTPPATNVLLMLYSNLSQEQRQLGGSTLLWEAESSWRAQEGQLSWEWGKRHRRHHLPDRSQLCRKVKFQVDFNLIGWGSWIIYPKQYNAYRCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSMLYVDNGRVLLDHHKDMIVEECGCL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Essential for mesoderm formation and axial patterning during embryonic development.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homodimer; disulfide-linked.

タンパク質ファミリー:

Belongs to the TGF-beta family.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

参考文献

1). The Photoperiod Regulates Granulosa Cell Apoptosis through the FSH-Nodal/ALK7 Signaling Pathway in Phodopus sungorus. Animals, 2022 (PubMed: 36552491) [IF=3.0]

Application: WB    Species: Mouse    Sample: Phodopus sungorus

Figure 7. Levels of the Nodal and ACVR1C protein expression in the ovaries of Phodopus sungorus under three photoperiod conditions. (A,B) Representative immunoblots of the key Nodal and ACVR1C proteins and internal reference proteins (α-tubulin and β-actin) in three different photoperiod groups. (C,D) Relative protein expression of Nodal and ACVR1C. Values are means ± SEM. n = 8. SD, short daylight; MD, moderate daylight; LD, long daylight.

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