製品: DAB2 Antibody
カタログ: DF7792
タンパク質の説明: Rabbit polyclonal antibody to DAB2
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse
予測: Pig, Bovine, Sheep, Rabbit, Dog, Xenopus
分子量: 105 kDa, 80 kDa; 82kD(Calculated).
ユニプロット: P98082
RRID: AB_2841249

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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(80%), Bovine(80%), Sheep(80%), Rabbit(80%), Dog(80%), Xenopus(83%)
クローナリティ:
Polyclonal
特異性:
DAB2 Antibody detects endogenous levels of total DAB2.
RRID:
AB_2841249
引用形式: Affinity Biosciences Cat# DF7792, RRID:AB_2841249.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

DAB 2; Dab, mitogen-responsive phosphoprotein, homolog 2 (Drosophila); DAB2; Differentially expressed in ovarian cancer 2; Differentially expressed protein 2; Disabled (Drosophila) homolog 2 (mitogen responsive phosphoprotein); Disabled homolog 2; Disabled homolog 2 mitogen responsive phosphoprotein (Drosophila); Disabled homolog 2 mitogen responsive phosphoprotein; Disabled, Drosophila, homolog of, 2; DOC 2; DOC2; FLJ26626; Mitogen responsive phosphoprotein;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P98082 DAB2_HUMAN:

Expressed in deep invaginations, inclusion cysts and the surface epithelial cells of the ovary. Also expressed in breast epithelial cells, spleen, thymus, prostate, testis, macrophages, fibroblasts, lung epithelial cells, placenta, brain stem, heart and small intestine. Expressed in kidney proximal tubular epithelial cells (at protein level).

タンパク質配列:
MSNEVETSATNGQPDQQAAPKAPSKKEKKKGPEKTDEYLLARFKGDGVKYKAKLIGIDDVPDARGDKMSQDSMMKLKGMAAAGRSQGQHKQRIWVNISLSGIKIIDEKTGVIEHEHPVNKISFIARDVTDNRAFGYVCGGEGQHQFFAIKTGQQAEPLVVDLKDLFQVIYNVKKKEEEKKKIEEASKAVENGSEALMILDDQTNKLKSGVDQMDLFGDMSTPPDLNSPTESKDILLVDLNSEIDTNQNSLRENPFLTNGITSCSLPRPTPQASFLPENAFSANLNFFPTPNPDPFRDDPFTQPDQSTPSSFDSLKSPDQKKENSSSSSTPLSNGPLNGDVDYFGQQFDQISNRTGKQEAQAGPWPFSSSQTQPAVRTQNGVSEREQNGFSVKSSPNPFVGSPPKGLSIQNGVKQDLESSVQSSPHDSIAIIPPPQSTKPGRGRRTAKSSANDLLASDIFAPPVSEPSGQASPTGQPTALQPNPLDLFKTSAPAPVGPLVGLGGVTVTLPQAGPWNTASLVFNQSPSMAPGAMMGGQPSGFSQPVIFGTSPAVSGWNQPSPFAASTPPPVPVVWGPSASVAPNAWSTTSPLGNPFQSNIFPAPAVSTQPPSMHSSLLVTPPQPPPRAGPPKDISSDAFTALDPLGDKEIKDVKEMFKDFQLRQPPAVPARKGEQTSSGTLSAFASYFNSKVGIPQENADHDDFDANQLLNKINEPPKPAPRQVSLPVTKSTDNAFENPFFKDSFGSSQASVASSQPVSSEMYRDPFGNPFA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Xenopus
83
Pig
80
Bovine
80
Sheep
80
Dog
80
Rabbit
80
Horse
70
Zebrafish
67
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P98082 基板として

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S24 Phosphorylation Q05655 (PRKCD) , P05129 (PRKCG) , P05771-2 (PRKCB)
K34 Ubiquitination
K53 Ubiquitination
R64 Methylation
K67 Acetylation
K67 Ubiquitination
K77 Ubiquitination
S98 Phosphorylation
S100 Phosphorylation
K108 Ubiquitination
K120 Ubiquitination
T151 Phosphorylation
K163 Methylation
Y170 Phosphorylation
K173 Methylation
S193 Phosphorylation
S208 Phosphorylation
T221 Phosphorylation
S227 Phosphorylation
T229 Phosphorylation
S231 Phosphorylation
S241 Phosphorylation
S264 Phosphorylation
T307 Phosphorylation
S313 Phosphorylation
K315 Ubiquitination
S316 Phosphorylation
K321 Ubiquitination
S324 Phosphorylation
S325 Phosphorylation
S326 Phosphorylation
S328 Phosphorylation
T329 Phosphorylation
S332 Phosphorylation
Y342 Phosphorylation
K356 Ubiquitination
S390 Phosphorylation
K392 Ubiquitination
S393 Phosphorylation
S394 Phosphorylation
S401 Phosphorylation
S407 Phosphorylation
S418 Phosphorylation
S419 Phosphorylation
S422 Phosphorylation
S423 Phosphorylation
S427 Phosphorylation
S436 Phosphorylation
T437 Phosphorylation
K438 Ubiquitination
S448 Phosphorylation
S449 Phosphorylation
T477 Phosphorylation
K646 Ubiquitination
K652 Ubiquitination
K670 Ubiquitination
S675 Phosphorylation
S684 Phosphorylation
Y685 Phosphorylation
S723 Phosphorylation
K728 Ubiquitination
S729 Phosphorylation
T730 Phosphorylation
Y761 Phosphorylation

研究背景

機能:

Adapter protein that functions as clathrin-associated sorting protein (CLASP) required for clathrin-mediated endocytosis of selected cargo proteins. Can bind and assemble clathrin, and binds simultaneously to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and cargos containing non-phosphorylated NPXY internalization motifs, such as the LDL receptor, to recruit them to clathrin-coated pits. Can function in clathrin-mediated endocytosis independently of the AP-2 complex. Involved in endocytosis of integrin beta-1; this function seems to redundant with the AP-2 complex and seems to require DAB2 binding to endocytosis accessory EH domain-containing proteins such as EPS15, EPS15L1 and ITSN1. Involved in endocytosis of cystic fibrosis transmembrane conductance regulator/CFTR. Involved in endocytosis of megalin/LRP2 lipoprotein receptor during embryonal development. Required for recycling of the TGF-beta receptor. Involved in CFTR trafficking to the late endosome. Involved in several receptor-mediated signaling pathways. Involved in TGF-beta receptor signaling and facilitates phosphorylation of the signal transducer SMAD2. Mediates TFG-beta-stimulated JNK activation. May inhibit the canoniocal Wnt/beta-catenin signaling pathway by stabilizing the beta-catenin destruction complex through a competing association with axin preventing its dephosphorylation through protein phosphatase 1 (PP1). Sequesters LRP6 towards clathrin-mediated endocytosis, leading to inhibition of Wnt/beta-catenin signaling. May activate non-canonical Wnt signaling. In cell surface growth factor/Ras signaling pathways proposed to inhibit ERK activation by interrupting the binding of GRB2 to SOS1 and to inhibit SRC by preventing its activating phosphorylation at 'Tyr-419'. Proposed to be involved in modulation of androgen receptor (AR) signaling mediated by SRC activation; seems to compete with AR for interaction with SRC. Plays a role in the CSF-1 signal transduction pathway. Plays a role in cellular differentiation. Involved in cell positioning and formation of visceral endoderm (VE) during embryogenesis and proposed to be required in the VE to respond to Nodal signaling coming from the epiblast. Required for the epithelial to mesenchymal transition, a process necessary for proper embryonic development. May be involved in myeloid cell differentiation and can induce macrophage adhesion and spreading. May act as a tumor suppressor.

PTMs:

Phosphorylated. Phosphorylation during mitosis is leading to membrane displacement (By similarity).

細胞の位置付け:

Cytoplasm. Cytoplasmic vesicle>Clathrin-coated vesicle membrane. Membrane>Clathrin-coated pit.
Note: Colocalizes with large insert-containing isoforms of MYO6 at clathrin-coated pits/vesicles. During mitosis is progressively displaced from the membrane and translocated to the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in deep invaginations, inclusion cysts and the surface epithelial cells of the ovary. Also expressed in breast epithelial cells, spleen, thymus, prostate, testis, macrophages, fibroblasts, lung epithelial cells, placenta, brain stem, heart and small intestine. Expressed in kidney proximal tubular epithelial cells (at protein level).

サブユニット構造:

Interacts (via NPXY motif) with DAB2 (via PID domain). Can interact (via PID domain) with LDLR, APP, APLP1 and APLP2, and weakly with INPP5D (via NPXY motifs); the interaction is impaired by tyrosine phosphorylation of the respective NPXY motifs. Can weakly interact (via PID domain) with LRP1 (via NPXY motif); the interaction is enhanced by tyrosine phosphorylation of the NPXY motif. Interacts with LRP2 (via NPXY motif); the interaction is not affected by tyrosine phosphorylation of the NPXY motif. Interacts with clathrin; in vitro can assemble clathrin triskelia into polyhedral coats. Interacts with AP2A2, ITGB1, ITGB3, ITGB5, PIAS2, DAB2IP, NOSTRIN, FCHO1, DVL3, EPS15, ITSN1 and EPS15L1. Interacts with SH3KBP1 (via SH3 domains). Interacts with GRB2; competes with SOS1 for binding to GRB2 and the interaction is enhanced by EGF and NT-3 stimulation. Interacts with MAP3K7; the interaction is induced by TGF-beta stimulation and may mediate TGF-beta stimulated JNK activation. Interacts with AXIN1 and PPP1CA; the interactions are mutually exclusive. Interacts with the globular tail of MYO6. Interacts (via DPF motifs) with FCHO2; the interaction is direct and required for DAB2-mediated LDLR endocytosis. Interacts with LRP6; the interaction involves LRP6 phosphorylation by CK2 and sequesters LRP6 towards clathrin-mediated endocytosis. Associates with the TGF-beta receptor complex (Probable). Interacts with SMAD2 and SMAD3; the interactions are enhanced upon TGF-beta stimulation. Interacts with GRB2; the interaction is enhanced by EGF and NT-3 stimulation. Interacts with SRC; the interaction is enhanced by EGF stimulation.

タンパク質ファミリー:

The PID domain binds to predominantly non-phosphorylated NPXY internalization motifs present in members of the LDLR and APP family; it also mediates simultaneous binding to phosphatidylinositol 4,5-bisphosphate.

The Asn-Pro-Phe (NPF) motifs, which are found in proteins involved in the endocytic pathway, mediate the interaction with the EH domain of EPS15, EPS15R and ITSN1.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

参考文献

1). DAB2 promotes pulmonary fibrosis and may act as an intermediate between IGF‑1R and PI3K/AKT signaling pathways. Experimental and Therapeutic Medicine, 2023 (PubMed: 37021069) [IF=2.7]

Application: WB    Species: Human    Sample: MRC-5 cells

Figure 2 Knockdown of DAB2 inhibits TGF-β1-induced proliferation of MRC-5 cells. Myofibroblast differentiation of MRC-5 cells (human embryonic lung fibroblasts) under TGF-β1 induction for 24 h. (A) Immunofluorescence staining of DAB2 in MRC-5 cells with TGF-β1 treatment (scale bar, 50 µm). (B) Relative mRNA level of DAB2 in TGF-β1-treated MRC-5 cells. ***P

Application: IF/ICC    Species: Human    Sample: MRC-5 cells

Figure 1 Expression of DAB2 is significantly upregulated in the bleomycin-induced fibrotic lung tissue. (A-C) Results from differential gene expression analysis based on Gene Expression Omnibus database. Lung samples from mice treated with saline (Control group) or bleomycin (BLM group) were set in GSE8553. (A) Volcano plot showing 244 differentially expressed genes with blue dots representing downregulated genes (Log2FC

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