CaMK1 alpha Antibody - #DF7805
| 製品: | CaMK1 alpha Antibody |
| カタログ: | DF7805 |
| タンパク質の説明: | Rabbit polyclonal antibody to CaMK1 alpha |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 41 kDa; 41kD(Calculated). |
| ユニプロット: | Q14012 |
| RRID: | AB_2841259 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7805, RRID:AB_2841259.
折りたたみ／展開
Calcium / calmodulin dependent protein kinase 1; Calcium / calmodulin dependent protein kinase I; Calcium / calmodulin dependent protein kinase type 1; Calcium/calmodulin dependent protein kinase 1; Calcium/calmodulin dependent protein kinase I; Calcium/calmodulin dependent protein kinase type 1; Calcium/calmodulin-dependent protein kinase type 1; CaM K1; CaM KI; CaM kinase 1 alpha; CAM kinase 1; CaM kinase I alpha; CaM kinase I; CaM-KI; CaMK 1; CAMK I; CaMK1 alpha; CAMK1; CAMK1 PEN; CaMKI alpha; CaMKI-alpha; KCC1A_HUMAN; MGC120317; MGC120318;
免疫原
Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.
- Q14012 KCC1A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLGAVEGPRWKQAEDIRDIYDFRDVLGTGAFSEVILAEDKRTQKLVAIKCIAKEALEGKEGSMENEIAVLHKIKHPNIVALDDIYESGGHLYLIMQLVSGGELFDRIVEKGFYTERDASRLIFQVLDAVKYLHDLGIVHRDLKPENLLYYSLDEDSKIMISDFGLSKMEDPGSVLSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVIAYILLCGYPPFYDENDAKLFEQILKAEYEFDSPYWDDISDSAKDFIRHLMEKDPEKRFTCEQALQHPWIAGDTALDKNIHQSVSEQIKKNFAKSKWKQAFNATAVVRHMRKLQLGTSQEGQGQTASHGELLTPVAGGPAAGCCCRDCCVEPGTELSPTLPHQL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14012 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y20 | Phosphorylation | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| Y131 | Phosphorylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| T177 | Phosphorylation | Q8N5S9 (CAMKK1) , Q96RR4 (CAMKK2) , Q14012 (CAMK1) | Uniprot |
| Y235 | Phosphorylation | Uniprot | |
| T323 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot |
PTMs - Q14012 酵素として
| Substrate | Site | Source |
|---|---|---|
| O43432 (EIF4G3) | S1156 | Uniprot |
| P05783 (KRT18) | S53 | Uniprot |
| P16220 (CREB1) | S133 | Uniprot |
| P17600 (SYN1) | S9 | Uniprot |
| P18846 (ATF1) | S63 | Uniprot |
| P24844 (MYL9) | S20 | Uniprot |
| P29475 (NOS1) | S746 | Uniprot |
| P29475-1 (NOS1) | S852 | Uniprot |
| P39019 (RPS19) | S59 | Uniprot |
| P46527 (CDKN1B) | T157 | Uniprot |
| P46527 (CDKN1B) | T198 | Uniprot |
| P49757 (NUMB) | S276 | Uniprot |
| P49757 (NUMB) | S295 | Uniprot |
| P57059 (SIK1) | T322 | Uniprot |
| Q05469 (LIPE) | S855 | Uniprot |
| Q14012 (CAMK1) | T177 | Uniprot |
| Q14155 (ARHGEF7) | S694 | Uniprot |
| Q7KZI7 (MARK2) | S91 | Uniprot |
| Q7KZI7 (MARK2) | S92 | Uniprot |
| Q7KZI7 (MARK2) | S93 | Uniprot |
| Q7KZI7 (MARK2) | T294 | Uniprot |
| Q9NP62 (GCM1) | S47 | Uniprot |
| Q9UKV0 (HDAC9) | S220 | Uniprot |
| Q9UKV0 (HDAC9) | S451 | Uniprot |
| Q9UQL6 (HDAC5) | S259 | Uniprot |
| Q9UQL6-3 (HDAC5) | S260 | Uniprot |
| Q9UQL6 (HDAC5) | S498 | Uniprot |
| Q9UQL6-3 (HDAC5) | S499 | Uniprot |
| Q9UQM7-2 (CAMK2A) | S332 | Uniprot |
研究背景
Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I.
Phosphorylated by CaMKK1 and CaMKK2 on Thr-177.
Polybiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL12), leading to proteasomal degradation.
Cytoplasm. Nucleus.
Note: Predominantly cytoplasmic.
Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.
Monomer. Interacts with XPO1 (By similarity). Interacts with MARK2, ARHGEF7/BETAPIX and GIT1.
The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
研究領域
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.