B-Myb Antibody - #DF7817

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製品: B-Myb Antibody
カタログ: DF7817
タンパク質の説明: Rabbit polyclonal antibody to B-Myb
アプリケーション: WB IHC
Cited expt.: WB, IHC
反応性: Human, Mouse
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 79kDa; 79kD(Calculated).
ユニプロット: P10244
RRID: AB_2841266

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%), Xenopus(80%)
クローナリティ:
Polyclonal
特異性:
B-Myb Antibody detects endogenous levels of total B-Myb.
RRID:
AB_2841266
引用形式: Affinity Biosciences Cat# DF7817, RRID:AB_2841266.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

B-Myb; BMyB; MGC15600; MYB L2; Myb related protein B; Myb-like protein 2; Myb-related protein B; MybB; MYBB_HUMAN; MYBL 2; Mybl2; v myb avian myeloblastosis viral oncogene homolog like 2; v myb myeloblastosis viral oncogene homolog (avian) like 2; v myb myeloblastosis viral oncogene homolog like 2;

免疫原

免疫原:

A synthesized peptide derived from human B-Myb, corresponding to a region within the internal amino acids.

Uniprot:

P10244(MYBB_HUMAN) >>Visit HPA database.

遺伝子(ID):
MYBL2(4605)
タンパク質配列:
MSRRTRCEDLDELHYQDTDSDVPEQRDSKCKVKWTHEEDEQLRALVRQFGQQDWKFLASHFPNRTDQQCQYRWLRVLNPDLVKGPWTKEEDQKVIELVKKYGTKQWTLIAKHLKGRLGKQCRERWHNHLNPEVKKSCWTEEEDRIICEAHKVLGNRWAEIAKMLPGRTDNAVKNHWNSTIKRKVDTGGFLSESKDCKPPVYLLLELEDKDGLQSAQPTEGQGSLLTNWPSVPPTIKEEENSEEELAAATTSKEQEPIGTDLDAVRTPEPLEEFPKREDQEGSPPETSLPYKWVVEAANLLIPAVGSSLSEALDLIESDPDAWCDLSKFDLPEEPSAEDSINNSLVQLQASHQQQVLPPRQPSALVPSVTEYRLDGHTISDLSRSSRGELIPISPSTEVGGSGIGTPPSVLKRQRKRRVALSPVTENSTSLSFLDSCNSLTPKSTPVKTLPFSPSQFLNFWNKQDTLELESPSLTSTPVCSQKVVVTTPLHRDKTPLHQKHAAFVTPDQKYSMDNTPHTPTPFKNALEKYGPLKPLPQTPHLEEDLKEVLRSEAGIELIIEDDIRPEKQKRKPGLRRSPIKKVRKSLALDIVDEDVKLMMSTLPKSLSLPTTAPSNSSSLTLSGIKEDNSLLNQGFLQAKPEKAAVAQKPRSHFTTPAPMSSAWKTVACGGTRDQLFMQEKARQLLGRLKPSHTSRTLILS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
80
Chicken
80
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.

PTMs:

Phosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

研究領域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

参考文献

1). LncRNA-MALAT1 Regulates Cancer Glucose Metabolism in Prostate Cancer via MYBL2/mTOR Axis. Oxidative Medicine and Cellular Longevity, 2022 (PubMed: 35557985)

Application: WB    Species: Mouse    Sample: Tumor tissues

Figure 2 MALAT1 regulates downstream mRNA MYBL2 and confer PCa progression. (a) QRT-PCR verified the MYBL2 levels in MALAT1 knockdown PC-3 cell line (left panel) and C4-2 cell line (right panel). (b) Western blot examines MYBL2 level in MALAT1 knockdown PC-3 and C4-2 cell lines (left panel), and quantification (right panel) of western blot result. (c) QRT-PCR displayed the knockdown efficiency in PC-3 and C4-2 by treating with MYBL2 siRNA. (d) Western blot tests MYBL2 level after treating PC-3 and C4-2 cell line with siMYBL2 (left panel) and quantification (right panel) of western blot result. (e) QRT-PCR examines the MALAT1 level in knockdown MYBL2 PC-3 cell line (left panel) and in C4-2 (right panel). (f) Representative images of EdU assay formed by the vector control PC-3 cell line and MALAT1 or MYBL2 knockdown cell lines. Scale bars: 50 μm. (h) Representative images of colonies formed by vector control PC-3 cell line and MALAT1 or MYBL2 knockdown cell lines. (g and i) Quantification of EdU assay (left panel) and colonies (right panel). All samples were normalized to GAPDH mRNA and protein levels. The error bars indicate SD (n = 3). Student t test was used. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

Application: IHC    Species: Mouse    Sample: Tumor tissues

Figure 5 MALAT1/MYBL2/mTOR axis regulates PCa progression and glucose metabolism in mouse model. (a) The tumorigenic ability of nude mouse model (n = 6) between injected knockdown MALAT1 PC-3 cell siMALAT1 group and vector control NC group. (b) Final tumor weight between siMALAT1 group and NC group. (c) Tumor volume growth between two groups. (d) Representative images of hematoxylin-eosin staining between two groups. Scale bars: 100 μm. (e–i) Representative images of immunohistochemistry of Ki67 (e), MYBL2 (f), mTOR (g), p-mTOR (h), and PKM2 (i) between siMALAT1 group and NC group. (j) Quantification of IHC results. Scale bars: 100 μm. The error bars indicate SD (n = 6). Student t test was used. ∗P < 0.05, ∗∗P < 0.01.

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