SPTAN1 Antibody - #DF7847

(2)  
製品: SPTAN1 Antibody
カタログ: DF7847
タンパク質の説明: Rabbit polyclonal antibody to SPTAN1
アプリケーション: WB IF/ICC
反応性: Human
予測: Bovine, Horse, Rabbit, Dog, Chicken
分子量: 285 kDa; 285kD(Calculated).
ユニプロット: Q13813
RRID: AB_2841284

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MS Report
HPLC Report
MSDS
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IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(91%)
クローナリティ:
Polyclonal
特異性:
SPTAN1 Antibody detects endogenous levels of total SPTAN1.
RRID:
AB_2841284
引用形式: Affinity Biosciences Cat# DF7847, RRID:AB_2841284.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

(ALPHA)II-SPECTRIN; Alpha II Spectrin; Alpha-II spectrin; brain; EIEE5; FLJ17738; FLJ44613; Fodrin alpha chain; Fodrin, alpha; NEAS; Non erythrocytic spectrin alpha; non-erythroid alpha chain; SPECA; Spectrin alpha chain; Spectrin alpha chain brain; Spectrin alpha non erythrocytic 1; Spectrin; Spectrin non erythroid alpha chain; Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin); Spectrin, nonerythroid, alpha subunit; Spna2; SPTA 2; SPTA2; SPTA2_HUMAN; SPTAN 1; SPTAN1;

免疫原

免疫原:
Uniprot:

Q13813(SPTN1_HUMAN) >>Visit HPA database.

遺伝子(ID):
SPTAN1(6709)
タンパク質配列:
MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Rabbit
100
Chicken
91
Pig
0
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13813 基板として

Site PTM Type Enzyme
M1 Acetylation
S33 Phosphorylation
T34 Phosphorylation
R51 Methylation
K58 Ubiquitination
K63 Ubiquitination
Y72 Phosphorylation
K73 Ubiquitination
K144 Ubiquitination
K150 Ubiquitination
K189 Ubiquitination
K190 Ubiquitination
T196 Phosphorylation
K214 Ubiquitination
K227 Ubiquitination
K241 Ubiquitination
S289 Phosphorylation
K310 Ubiquitination
R320 Methylation
S328 Phosphorylation
T330 Phosphorylation
K335 Ubiquitination
S375 Phosphorylation
S391 Phosphorylation
R402 Methylation
S416 Phosphorylation
S419 Phosphorylation
S423 Phosphorylation
Y433 Phosphorylation
S435 Phosphorylation
K441 Ubiquitination
R459 Methylation
K514 Ubiquitination
S517 Phosphorylation
K522 Ubiquitination
S553 Phosphorylation
S572 Phosphorylation
K592 Acetylation
T595 Phosphorylation
Y601 Phosphorylation
K602 Ubiquitination
K610 Ubiquitination
K613 Acetylation
K613 Ubiquitination
K633 Ubiquitination
K637 Acetylation
K637 Ubiquitination
Y644 Phosphorylation
K646 Ubiquitination
T667 Phosphorylation
K718 Ubiquitination
K719 Ubiquitination
R733 Methylation
T738 Phosphorylation
K757 Ubiquitination
K758 Ubiquitination
Y766 Phosphorylation
K779 Acetylation
S783 Phosphorylation
K803 Ubiquitination
S809 Phosphorylation
T810 Phosphorylation
K814 Ubiquitination
K824 Ubiquitination
K825 Ubiquitination
K864 Ubiquitination
K909 Ubiquitination
K920 Ubiquitination
S937 Phosphorylation
Y942 Phosphorylation
S944 Phosphorylation
Y976 Phosphorylation
Y978 Phosphorylation
S982 Phosphorylation
K990 Ubiquitination
T995 Phosphorylation
S999 Phosphorylation
T1000 Phosphorylation
Y1020 Phosphorylation
K1022 Acetylation
K1023 Acetylation
S1029 Phosphorylation
S1031 Phosphorylation
S1041 Phosphorylation
T1053 Phosphorylation
K1057 Ubiquitination
S1061 Phosphorylation
S1063 Phosphorylation
K1067 Ubiquitination
Y1073 Phosphorylation P12931 (SRC)
S1075 Phosphorylation
K1082 Ubiquitination
K1093 Acetylation
T1115 Phosphorylation
S1116 Phosphorylation
K1131 Ubiquitination
K1132 Ubiquitination
K1138 Methylation
K1138 Ubiquitination
S1159 Phosphorylation
Y1176 Phosphorylation P12931 (SRC) , P06239 (LCK)
T1184 Phosphorylation
S1186 Phosphorylation
K1187 Ubiquitination
T1188 Phosphorylation
S1190 Phosphorylation
K1193 Ubiquitination
S1194 Phosphorylation
T1204 Phosphorylation
S1207 Phosphorylation
K1209 Ubiquitination
S1217 Phosphorylation
S1226 Phosphorylation
S1231 Phosphorylation
K1246 Ubiquitination
K1252 Ubiquitination
Y1261 Phosphorylation
K1274 Ubiquitination
K1288 Ubiquitination
S1291 Phosphorylation
S1302 Phosphorylation
S1306 Phosphorylation
K1313 Ubiquitination
S1322 Phosphorylation
S1323 Phosphorylation
K1326 Ubiquitination
K1334 Acetylation
S1338 Phosphorylation
S1346 Phosphorylation
S1363 Phosphorylation
K1369 Acetylation
T1386 Phosphorylation
Y1411 Phosphorylation
S1413 Phosphorylation
K1419 Ubiquitination
R1464 Methylation
K1473 Ubiquitination
S1476 Phosphorylation
S1479 Phosphorylation
K1485 Ubiquitination
K1519 Acetylation
K1519 Ubiquitination
R1532 Methylation
S1550 Phosphorylation
T1552 Phosphorylation
S1557 Phosphorylation
S1578 Phosphorylation
Y1579 Phosphorylation
K1580 Ubiquitination
K1588 Ubiquitination
K1591 Acetylation
S1615 Phosphorylation
S1625 Phosphorylation
K1630 Ubiquitination
S1647 Phosphorylation
K1659 Ubiquitination
S1692 Phosphorylation
K1698 Ubiquitination
K1699 Ubiquitination
S1708 Phosphorylation
K1715 Ubiquitination
S1719 Phosphorylation
S1751 Phosphorylation
S1755 Phosphorylation
S1778 Phosphorylation
Y1792 Phosphorylation
T1797 Phosphorylation
K1829 Ubiquitination
K1838 Ubiquitination
K1853 Ubiquitination
K1856 Ubiquitination
Y1898 Phosphorylation
K1911 Acetylation
K1911 Ubiquitination
K1923 Ubiquitination
S1959 Phosphorylation
K1963 Ubiquitination
K1995 Ubiquitination
S2009 Phosphorylation
S2010 Phosphorylation
T2016 Phosphorylation
T2020 Phosphorylation
K2040 Ubiquitination
K2047 Acetylation
K2047 Ubiquitination
K2052 Acetylation
K2052 Ubiquitination
S2066 Phosphorylation
S2072 Phosphorylation
K2078 Ubiquitination
S2084 Phosphorylation
T2095 Phosphorylation
K2098 Acetylation
K2099 Ubiquitination
R2137 Methylation
S2138 Phosphorylation
S2139 Phosphorylation
S2159 Phosphorylation
K2187 Ubiquitination
K2195 Acetylation
K2264 Ubiquitination
K2281 Ubiquitination
S2340 Phosphorylation
K2404 Ubiquitination
S2411 Phosphorylation
K2421 Acetylation
K2421 Ubiquitination
Y2423 Phosphorylation
T2425 Phosphorylation
K2426 Acetylation
K2426 Ubiquitination
Y2430 Phosphorylation
T2434 Phosphorylation
Y2440 Phosphorylation
K2446 Acetylation
K2452 Ubiquitination
T2458 Phosphorylation
Y2462 Phosphorylation

研究背景

機能:

Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.

PTMs:

Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.

細胞の位置付け:

Cytoplasm>Cytoskeleton. Cytoplasm>Cell cortex.
Note: Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Like erythrocyte spectrin, the spectrin-like proteins are capable of forming dimers which can further associate to tetramers. Interacts (via C-terminal spectrin repeats) with TRPC4. Interacts with CALM and EMD. Interacts with isoform 1 of ACP1. Identified in a complex with ACTN4, CASK, IQGAP1, MAGI2, NPHS1 and SPTBN1. Interacts with SHANK3 (via ANK repeats).

タンパク質ファミリー:

Belongs to the spectrin family.

研究領域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

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