NRP1 Antibody - #DF7877
(1)
(4)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7877, RRID:AB_2841305.
折りたたみ／展開
A5 protein; BDCA4; BLOOD DENDRITIC CELL ANTIGEN 4; CD304; Neuropilin-1; Neuropilin1; NP1; NPN1; NRP 1; NRP; NRP1; NRP1_HUMAN; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165R;
免疫原
The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
- O14786 NRP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MERGLPLLCAVLALVLAPAGAFRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFVPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEALGMESGEIHSDQITASSQYSTNWSAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYKIDVSSNGEDWITIKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRIKPATWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPAPHSYINEWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSEFPTYGFNCEFGWGSHKTFCHWEHDNHVQLKWSVLTSKTGPIQDHTGDGNFIYSQADENQKGKVARLVSPVVYSQNSAHCMTFWYHMSGSHVGTLRVKLRYQKPEEYDQLVWMAIGHQGDHWKEGRVLLHKSLKLYQVIFEGEIGKGNLGGIAVDDISINNHISQEDCAKPADLDKKNPEIKIDETGSTPGYEGEGEGDKNISRKPGNVLKTLDPILITIIAMSALGVLLGAVCGVVLYCACWHNGMSERNLSALENYNFELVDGVKLKKDKLNTQSTYSEA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O14786 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T30 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| N150 | N-Glycosylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| N261 | N-Glycosylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| Y297 | Phosphorylation | Uniprot | |
| Y310 | Phosphorylation | Uniprot | |
| T316 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| Y322 | Phosphorylation | Uniprot | |
| Y353 | Phosphorylation | Uniprot | |
| Y354 | Phosphorylation | Uniprot | |
| Y358 | Phosphorylation | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| T448 | Phosphorylation | Uniprot | |
| N522 | N-Glycosylation | Uniprot | |
| S524 | Phosphorylation | Uniprot | |
| S612 | O-Glycosylation | Uniprot | |
| T827 | O-Glycosylation | Uniprot | |
| T827 | Phosphorylation | Uniprot | |
| S829 | O-Glycosylation | Uniprot | |
| T830 | O-Glycosylation | Uniprot | |
| S844 | O-Glycosylation | Uniprot | |
| S894 | Phosphorylation | Uniprot | |
| Y899 | Phosphorylation | Uniprot | |
| K908 | Ubiquitination | Uniprot | |
| K913 | Ubiquitination | Uniprot | |
| T919 | Phosphorylation | Uniprot | |
| Y920 | Phosphorylation | Uniprot | |
| S921 | Phosphorylation | Uniprot |
研究背景
Receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF165 isoform of VEGFA and VEGFB. Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR.
Binds VEGF-165 and may inhibit its binding to cells. May induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
Secreted.
Mitochondrion membrane>Single-pass type I membrane protein. Cell membrane>Single-pass type I membrane protein. Cytoplasm.
The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
Homodimer, and heterodimer with NRP2. Interacts with FER (By similarity). Interacts with PLXNB1. Interacts with VEGFA. Interacts with ABCB8/MITOSUR in mitochondria.
The tandem CUB domains mediate binding to semaphorin, while the tandem F5/8 domains are responsible for heparin and VEGF binding.
Belongs to the neuropilin family.
研究領域
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Organismal Systems > Development > Axon guidance. (View pathway)
参考文献
Application: WB Species: human Sample: gastric cancer cells
Application: WB Species: Human Sample: gastric cancer tissue
Application: IHC Species: Human Sample: gastric cancer cell
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