AP2S1 Antibody - #DF7883
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7883, RRID:AB_2841307.
折りたたみ／展開
Adapter-related protein complex 2 sigma subunit; Adaptor protein complex AP 2 subunit sigma; Adaptor protein complex AP-2 subunit sigma; Adaptor protein complex AP2 subunit sigma; Adaptor related protein complex 2 sigma 1 subunit; AI043088; AP 17; AP 2 complex subunit sigma 1; AP-2 complex subunit sigma; AP17; AP17 delta; AP2 complex subunit sigma 1; Ap2s1; AP2S1_HUMAN; CLAPS 2; CLAPS2; Clathrin adaptor complex AP2, sigma subunit; Clathrin adaptor protein AP17; Clathrin assembly protein 2 small chain; Clathrin associated/assembly/adaptor protein small 2; Clathrin associated/assembly/adaptor protein small 2, 17-KD; Clathrin associated/assembly/adaptor protein, small 2 (17kD); Clathrin coat assembly protein AP17; Clathrin coat associated protein AP17; Clathrin coat-associated protein AP17; HA2 17 kDa subunit; MGC62945; Plasma membrane adaptor AP 2 17 kDa protein; Plasma membrane adaptor AP-2 17 kDa protein; Plasma membrane adaptor AP2 17 kDa protein; Sigma adaptin 3b; Sigma2 adaptin; Sigma2-adaptin;
免疫原
- P53680 AP2S1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MIRFILIQNRAGKTRLAKWYMQFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNFKIIYRRYAGLYFCICVDVNDNNLAYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFLAGEIRETSQTKVLKQLLMLQSLE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P53680 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K18 | Ubiquitination | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| T129 | Phosphorylation | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| S140 | Phosphorylation | Uniprot |
研究背景
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity). May also play a role in extracellular calcium homeostasis.
Cell membrane. Membrane>Coated pit>Peripheral membrane protein>Cytoplasmic side.
Note: AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1).
Belongs to the adaptor complexes small subunit family.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Organismal Systems > Nervous system > Synaptic vesicle cycle.
· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.
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