製品: Hemoglobin subunit alpha Antibody
カタログ: DF7893
タンパク質の説明: Rabbit polyclonal antibody to Hemoglobin subunit alpha
アプリケーション: WB IF/ICC
反応性: Human
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
分子量: 15 kDa, 25 kDa; 15kD(Calculated).
ユニプロット: P69905
RRID: AB_2841314

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:200-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Dog(92%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
Hemoglobin subunit alpha Antibody detects endogenous levels of total Hemoglobin subunit alpha.
RRID:
AB_2841314
引用形式: Affinity Biosciences Cat# DF7893, RRID:AB_2841314.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Alpha 1 globin; Alpha globin; Alpha one globin; Alpha-globin; HBA_HUMAN; HBA1; HBA2; Hemoglobin alpha 1; Hemoglobin alpha 1 chain; Hemoglobin alpha 1 globin chain; Hemoglobin alpha 2; Hemoglobin alpha chain; Hemoglobin subunit alpha; MGC126895; MGC126897;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P69905 HBA_HUMAN:

Red blood cells.

タンパク質配列:
MVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYR

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Dog
92
Zebrafish
82
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P69905 基板として

Site PTM Type Enzyme
S4 O-Glycosylation
S4 Phosphorylation
K8 Acetylation
T9 Phosphorylation
K12 Acetylation
K17 Acetylation
K17 Ubiquitination
Y25 Phosphorylation
S36 O-Glycosylation
S36 Phosphorylation
T39 Phosphorylation
T40 Phosphorylation
K41 Acetylation
K41 Ubiquitination
T42 Phosphorylation
Y43 Phosphorylation
S50 Phosphorylation
S53 Phosphorylation
K57 Acetylation
K57 Ubiquitination
K61 Acetylation
K61 Ubiquitination
K62 Acetylation
S82 Phosphorylation
K91 Acetylation
K100 Acetylation
C105 S-Nitrosylation
T109 Phosphorylation
S132 Phosphorylation
S134 O-Glycosylation
T135 Phosphorylation
T138 Phosphorylation
S139 Phosphorylation
K140 Acetylation
Y141 Phosphorylation

研究背景

機能:

Involved in oxygen transport from the lung to the various peripheral tissues.

PTMs:

The initiator Met is not cleaved in variant Thionville and is acetylated.

組織特異性:

Red blood cells.

サブユニット構造:

Heterotetramer of two alpha chains and two beta chains in adult hemoglobin A (HbA); two alpha chains and two delta chains in adult hemoglobin A2 (HbA2); two alpha chains and two epsilon chains in early embryonic hemoglobin Gower-2; two alpha chains and two gamma chains in fetal hemoglobin F (HbF).

(Microbial infection) Interacts with Staphylococcus aureus protein isdB.

タンパク質ファミリー:

Belongs to the globin family.

研究領域

· Human Diseases > Infectious diseases: Parasitic > African trypanosomiasis.

· Human Diseases > Infectious diseases: Parasitic > Malaria.

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