APOA2 Antibody - #DF7905

(2)   (3)  
製品: APOA2 Antibody
カタログ: DF7905
タンパク質の説明: Rabbit polyclonal antibody to APOA2
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Bovine, Sheep, Dog
分子量: 11 kDa; 11kD(Calculated).
ユニプロット: P02652
RRID: AB_2841322

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(80%), Sheep(80%), Dog(83%)
クローナリティ:
Polyclonal
特異性:
APOA2 Antibody detects endogenous levels of total APOA2.
RRID:
AB_2841322
引用形式: Affinity Biosciences Cat# DF7905, RRID:AB_2841322.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

APO A2; Apo AII; Apo-AII; APOA 2; ApoA II; ApoA-II; APOA2; APOA2_HUMAN; APOAII; Apolipoprotein A II; Apolipoprotein A-II(1-76); Apolipoprotein A2; Apolipoprotein AII; ApolipoproteinA II; OTTHUMP00000032244;

免疫原

免疫原:
Uniprot:

P02652(APOA2_HUMAN) >>Visit HPA database.

遺伝子(ID):
APOA2(336)
発現特異性:
P02652 APOA2_HUMAN:

Plasma; synthesized in the liver and intestine.

タンパク質配列:
MKLLAATVLLLTICSLEGALVRRQAKEPCVESLVSQYFQTVTDYGKDLMEKVKSPELQAEAKSYFEKSKEQLTPLIKKAGTELVNFLSYFVELGTQPATQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
83
Bovine
80
Sheep
80
Rabbit
75
Horse
67
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02652 基板として

Site PTM Type Enzyme
S32 Phosphorylation
S35 Phosphorylation
Y37 Phosphorylation
T42 O-Glycosylation
S54 Phosphorylation
K67 Acetylation
S68 Phosphorylation

研究背景

機能:

May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.

PTMs:

Phosphorylation sites are present in the extracellular medium.

Apolipoprotein A-II is O-glycosylated.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Plasma; synthesized in the liver and intestine.

サブユニット構造:

Monomer. Homodimer; disulfide-linked. Also forms a disulfide-linked heterodimer with APOD.Interacts with APOA1BP and NDRG1.

(Microbial infection) Interacts with HCV core protein.

タンパク質ファミリー:

Belongs to the apolipoprotein A2 family.

研究領域

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Digestive system > Cholesterol metabolism.

参考文献

1). Neural stem cell-derived exosomes regulate neural stem cell differentiation through miR-9-Hes1 axis. Frontiers in Cell and Developmental Biology, 2021 (PubMed: 34055767) [IF=5.5]

Application: WB    Species: Mouse    Sample: neural stem cells (NSC)

FIGURE 1 Characterization of exosomes (EXOs). (A) Purified exosomes were observed under transmission electron microscopy (TEM) using negative staining. (B) The levels of positive exosomal markers TSG101, CD9, and Flotillin-1 in protein lysates of neural stem cells (NSC)-derived exosome pellets were determined by western blotting. (C) The levels of negative exosomal markers APOA1 and APOA2 in protein lysates of NSC-derived exosome pellets and NSCs were determined by western blotting. (D,E) Particle-size distribution of exosomes was determined by NanoSight analysis (NTA) in panel (D) and NanoFCM in panel (E) technologies. Scale bar 200 nm in panel (A).

Application: WB    Species: mouse    Sample: NSCs

FIGURE 1 | Characterization of exosomes (EXOs). (C) The levels of negative exosomal markers APOA1 and APOA2 in protein lysates of NSC-derived exosome pellets and NSCs were determined by western blotting.
2). Neural stem cell-derived exosomal FTO protects neuron from microglial inflammatory injury by inhibiting microglia NRF2 mRNA m6A modification. Journal of neurogenetics, 2023 (PubMed: 37812019) [IF=1.9]

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