TMPRSS2 Antibody - #DF7917

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製品: TMPRSS2 Antibody
カタログ: DF7917
タンパク質の説明: Rabbit polyclonal antibody to TMPRSS2
アプリケーション: WB IHC
反応性: Human, Mouse
予測: Pig, Bovine, Horse, Dog
分子量: 54 kDa; 54kD(Calculated).
ユニプロット: O15393
RRID: AB_2841332

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(92%), Bovine(100%), Horse(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
TMPRSS2 Antibody detects endogenous levels of total TMPRSS2.
RRID:
AB_2841332
引用形式: Affinity Biosciences Cat# DF7917, RRID:AB_2841332.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

D16Ertd61e; Epitheliasin; FLJ41954; MGC6821; PP9284; PRSS10; Serine protease 10; TMPRSS2; TMPRSS2 ERG FUSION GENE, INCLUDED; TMPRSS2 ETV1 FUSION GENE, INCLUDED; TMPS2_HUMAN; Transmembrane protease serine 2 catalytic chain; Transmembrane protease, serine 2; Transmembrane protease, serine 2, EC 3.4.219;

免疫原

免疫原:
Uniprot:

O15393(TMPS2_HUMAN) >>Visit HPA database.

遺伝子(ID):
TMPRSS2(7113)
発現特異性:
O15393 TMPS2_HUMAN:

Highly expressed in prostate epithelial cells and in prostate cancers. Expressed in type II pneumocytes in the lung (at protein level). Expressed strongly in small intestine. Also expressed in colon, stomach and salivary gland.

タンパク質配列:
MALNSGSPPAIGPYYENHGYQPENPYPAQPTVVPTVYEVHPAQYYPSPVPQYAPRVLTQASNPVVCTQPKSPSGTVCTSKTKKALCITLTLGTFLVGAALAAGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYSSQRKSWHPVCQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSRQSRIVGGESALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTKNNDIALMKLQKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGDSGGPLVTSKNNIWWLIGDTSWGSGCAKAYRPGVYGNVMVFTDWIYRQMRADG

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Pig
92
Chicken
58
Sheep
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15393 基板として

Site PTM Type Enzyme
Y44 Phosphorylation
Y45 Phosphorylation
Y52 Phosphorylation
T58 Phosphorylation
S61 Phosphorylation
T341 Phosphorylation

研究背景

機能:

Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions; spike proteins are synthesized and maintained in precursor intermediate folding states and proteolysis permits the refolding and energy release required to create stable virus-cell linkages and membrane coalescence. Facilitates human SARS coronavirus (SARS-CoV) infection via two independent mechanisms, proteolytic cleavage of ACE2, which might promote viral uptake, and cleavage of coronavirus spike glycoprotein which activates the glycoprotein for cathepsin L-independent host cell entry. Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9); involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.

PTMs:

Proteolytically processed; by an autocatalytic mechanism.

細胞の位置付け:

Cell membrane>Single-pass type II membrane protein.

Secreted.
Note: Activated by cleavage and secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in prostate epithelial cells and in prostate cancers. Expressed in type II pneumocytes in the lung (at protein level). Expressed strongly in small intestine. Also expressed in colon, stomach and salivary gland.

サブユニット構造:

The catalytically active form interacts with ACE2.

タンパク質ファミリー:

Belongs to the peptidase S1 family.

研究領域

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

参考文献

1). Revealing the mechanism of ethyl acetate extracts of Semen Impatientis against prostate cancer based on network pharmacology and transcriptomics. Journal of ethnopharmacology, 2024 (PubMed: 38643863) [IF=5.4]

Application: WB    Species: Human    Sample:

Fig. 6. Network pharmacology analysis and experiments were utilized to explore the molecular mechanism of EAESI in PCa. (A) Disease-related targets for PCa were selected from DrugBank, GeneCards, OMIM, and the TTD. (B) Venn diagram showing the intersection of the drug targets and the disease-related targets. (C) A compound–target network was constructed. (D) The common targets of Semen Impatientis and PCa were used to construct the PPI network. (E) Topological analysis of the PPI network was employed to identify the hub genes. (F–G) The impacts of EAESI on the expression and transcriptional activity of AR in LNCaP and 22RV1 cells were evaluated by RT‒qPCR (F) and Western blot (G) assays. The data are presented as the means ± SDs. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the 0 mg/mL group.

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