製品: EHD1 Antibody
カタログ: DF7948
タンパク質の説明: Rabbit polyclonal antibody to EHD1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Xenopus
分子量: 61 kDa; 61kD(Calculated).
ユニプロット: Q9H4M9
RRID: AB_2841350

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Xenopus(92%)
クローナリティ:
Polyclonal
特異性:
EHD1 Antibody detects endogenous levels of total EHD1.
RRID:
AB_2841350
引用形式: Affinity Biosciences Cat# DF7948, RRID:AB_2841350.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

CDABP0131; EH domain containing 1; EH domain-containing protein 1; EHD1; EHD1_HUMAN; FLJ42622; FLJ44618; H PAST; hPAST1; PAST; PAST homolog 1; PAST1; Testilin;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q9H4M9 EHD1_HUMAN:

Highly expressed in testis.

タンパク質配列:
MFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRHLIEQDFPGMRIGPEPTTDSFIAVMHGPTEGVVPGNALVVDPRRPFRKLNAFGNAFLNRFMCAQLPNPVLDSISIIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKIRVVLNKADQIETQQLMRVYGALMWSLGKIINTPEVVRVYIGSFWSHPLLIPDNRKLFEAEEQDLFKDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPNVFGKESKKKELVNNLGEIYQKIEREHQISPGDFPSLRKMQELLQTQDFSKFQALKPKLLDTVDDMLANDIARLMVMVRQEESLMPSQVVKGGAFDGTMNGPFGHGYGEGAGEGIDDVEWVVGKDKPTYDEIFYTLSPVNGKITGANAKKEMVKSKLPNTVLGKIWKLADVDKDGLLDDEEFALANHLIKVKLEGHELPADLPPHLVPPSKRRHE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Xenopus
92
Dog
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H4M9 基板として

Site PTM Type Enzyme
M1 Acetylation
K7 Ubiquitination
K13 Ubiquitination
Y29 Phosphorylation
K32 Ubiquitination
Y40 Phosphorylation
T69 Phosphorylation
K124 Ubiquitination
K162 Ubiquitination
K205 Ubiquitination
K208 Sumoylation
K220 Ubiquitination
T226 Phosphorylation
T246 Phosphorylation
K280 Ubiquitination
S284 Phosphorylation
K293 Ubiquitination
K299 Ubiquitination
K316 Ubiquitination
K324 Acetylation
K324 Ubiquitination
K327 Acetylation
Y339 Phosphorylation
K341 Ubiquitination
S349 Phosphorylation
S355 Phosphorylation
K358 Ubiquitination
K370 Ubiquitination
K375 Ubiquitination
T417 Phosphorylation
Y426 Phosphorylation
T447 Phosphorylation
Y448 Phosphorylation
Y453 Phosphorylation
T454 Phosphorylation
S456 Phosphorylation
K475 Ubiquitination
T479 Phosphorylation
K492 Ubiquitination
K509 Ubiquitination

研究背景

機能:

ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes. Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth (By similarity). Plays a role in myoblast fusion (By similarity). Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing (By similarity). Plays a role in the formation of the ciliary vesicle (CV), an early step in cilium biogenesis. Proposed to be required for the fusion of distal appendage vesicles (DAVs) to form the CV by recruiting SNARE complex component SNAP29. Is required for recruitment of transition zone proteins CEP290, RPGRIP1L, TMEM67 and B9D2, and of IFT20 following DAV reorganization before Rab8-dependent ciliary membrane extension. Required for the loss of CCP110 form the mother centriole essential for the maturation of the basal body during ciliogenesis.

細胞の位置付け:

Recycling endosome membrane>Peripheral membrane protein>Cytoplasmic side. Early endosome membrane>Peripheral membrane protein>Cytoplasmic side. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Cilium membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Preferentially associates with tubular recycling endosomes (PubMed:15020713, PubMed:17233914, PubMed:19864458, PubMed:23596323). Colocalizes with FER1L5 at plasma membrane in myoblasts and myotubes (By similarity). Localizes to the ciliary pocket from where the cilium protrudes (PubMed:25686250). Colocalizes with BACE1 in tubulovesicular cytoplasmic membranes. Colocalizes with BACE1 and APP amyloid beta proteins in hippocampal mossy fiber terminals (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in testis.

サブユニット構造:

Homooligomer, and heterooligomer with EHD2, EHD3 and EHD4, ATP-binding is required for heterooligomerization. Interacts (via EH domain) with MICALL1 (via NPF1 motif); the interaction is direct and recruits EHD1 to membranes. Interacts with RAB35; the interaction is indirect through MICALL1 and recruits EHD1 to membranes (By similarity). Interacts (via EH domain) with PACSIN2 (via NPF motifs); regulates localization to tubular recycling endosome membranes. Interacts with PACSIN1 (By similarity). Interacts with RAB8A. Interacts with FER1L5 (via second C2 domain) (By similarity). Interacts with MYOF (By similarity). Interacts with ZFYVE20. Interacts (via EH domain) with RAB11FIP2.

タンパク質ファミリー:

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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