EHD1 Antibody - #DF7948
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7948, RRID:AB_2841350.
折りたたみ/展開
CDABP0131; EH domain containing 1; EH domain-containing protein 1; EHD1; EHD1_HUMAN; FLJ42622; FLJ44618; H PAST; hPAST1; PAST; PAST homolog 1; PAST1; Testilin;
免疫原
- Q9H4M9 EHD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRHLIEQDFPGMRIGPEPTTDSFIAVMHGPTEGVVPGNALVVDPRRPFRKLNAFGNAFLNRFMCAQLPNPVLDSISIIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKIRVVLNKADQIETQQLMRVYGALMWSLGKIINTPEVVRVYIGSFWSHPLLIPDNRKLFEAEEQDLFKDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPNVFGKESKKKELVNNLGEIYQKIEREHQISPGDFPSLRKMQELLQTQDFSKFQALKPKLLDTVDDMLANDIARLMVMVRQEESLMPSQVVKGGAFDGTMNGPFGHGYGEGAGEGIDDVEWVVGKDKPTYDEIFYTLSPVNGKITGANAKKEMVKSKLPNTVLGKIWKLADVDKDGLLDDEEFALANHLIKVKLEGHELPADLPPHLVPPSKRRHE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H4M9 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
M1 | Acetylation | Uniprot | |
K7 | Ubiquitination | Uniprot | |
K13 | Ubiquitination | Uniprot | |
Y29 | Phosphorylation | Uniprot | |
K32 | Ubiquitination | Uniprot | |
Y40 | Phosphorylation | Uniprot | |
T69 | Phosphorylation | Uniprot | |
K124 | Ubiquitination | Uniprot | |
K162 | Ubiquitination | Uniprot | |
K205 | Ubiquitination | Uniprot | |
K208 | Sumoylation | Uniprot | |
K220 | Ubiquitination | Uniprot | |
T226 | Phosphorylation | Uniprot | |
T246 | Phosphorylation | Uniprot | |
K280 | Ubiquitination | Uniprot | |
S284 | Phosphorylation | Uniprot | |
K293 | Ubiquitination | Uniprot | |
K299 | Ubiquitination | Uniprot | |
K316 | Ubiquitination | Uniprot | |
K324 | Acetylation | Uniprot | |
K324 | Ubiquitination | Uniprot | |
K327 | Acetylation | Uniprot | |
Y339 | Phosphorylation | Uniprot | |
K341 | Ubiquitination | Uniprot | |
S349 | Phosphorylation | Uniprot | |
S355 | Phosphorylation | Uniprot | |
K358 | Ubiquitination | Uniprot | |
K370 | Ubiquitination | Uniprot | |
K375 | Ubiquitination | Uniprot | |
T417 | Phosphorylation | Uniprot | |
Y426 | Phosphorylation | Uniprot | |
T447 | Phosphorylation | Uniprot | |
Y448 | Phosphorylation | Uniprot | |
Y453 | Phosphorylation | Uniprot | |
T454 | Phosphorylation | Uniprot | |
S456 | Phosphorylation | Uniprot | |
K475 | Ubiquitination | Uniprot | |
T479 | Phosphorylation | Uniprot | |
K492 | Ubiquitination | Uniprot | |
K509 | Ubiquitination | Uniprot |
研究背景
ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes. Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth (By similarity). Plays a role in myoblast fusion (By similarity). Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing (By similarity). Plays a role in the formation of the ciliary vesicle (CV), an early step in cilium biogenesis. Proposed to be required for the fusion of distal appendage vesicles (DAVs) to form the CV by recruiting SNARE complex component SNAP29. Is required for recruitment of transition zone proteins CEP290, RPGRIP1L, TMEM67 and B9D2, and of IFT20 following DAV reorganization before Rab8-dependent ciliary membrane extension. Required for the loss of CCP110 form the mother centriole essential for the maturation of the basal body during ciliogenesis.
Recycling endosome membrane>Peripheral membrane protein>Cytoplasmic side. Early endosome membrane>Peripheral membrane protein>Cytoplasmic side. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Cilium membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Preferentially associates with tubular recycling endosomes (PubMed:15020713, PubMed:17233914, PubMed:19864458, PubMed:23596323). Colocalizes with FER1L5 at plasma membrane in myoblasts and myotubes (By similarity). Localizes to the ciliary pocket from where the cilium protrudes (PubMed:25686250). Colocalizes with BACE1 in tubulovesicular cytoplasmic membranes. Colocalizes with BACE1 and APP amyloid beta proteins in hippocampal mossy fiber terminals (By similarity).
Highly expressed in testis.
Homooligomer, and heterooligomer with EHD2, EHD3 and EHD4, ATP-binding is required for heterooligomerization. Interacts (via EH domain) with MICALL1 (via NPF1 motif); the interaction is direct and recruits EHD1 to membranes. Interacts with RAB35; the interaction is indirect through MICALL1 and recruits EHD1 to membranes (By similarity). Interacts (via EH domain) with PACSIN2 (via NPF motifs); regulates localization to tubular recycling endosome membranes. Interacts with PACSIN1 (By similarity). Interacts with RAB8A. Interacts with FER1L5 (via second C2 domain) (By similarity). Interacts with MYOF (By similarity). Interacts with ZFYVE20. Interacts (via EH domain) with RAB11FIP2.
The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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