USP18 Antibody - #DF7968

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製品: USP18 Antibody
カタログ: DF7968
タンパク質の説明: Rabbit polyclonal antibody to USP18
アプリケーション: WB
反応性: Human
分子量: 43 kDa.; 43kD(Calculated).
ユニプロット: Q9UMW8
RRID: AB_2841360

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Polyclonal
特異性:
USP18 Antibody detects endogenous levels of total USP18.
RRID:
AB_2841360
引用形式: Affinity Biosciences Cat# DF7968, RRID:AB_2841360.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

43 kDa ISG15 specific protease; 43 kDa ISG15-specific protease; EC 3.1.2.; hUBP43; Interferon Stimulated Gene 43 kD; ISG15 Specific Processing Protease; ISG15-specific-processing protease; ISG43; Ubiquitin Specific Peptidase 18; Ubiquitin Specific Protease 18 43 kD; Ubiquitin Specific Protease 18; Ubl carboxyl terminal hydrolase 18; Ubl carboxyl-terminal hydrolase 18; Ubl thioesterase 18; Ubl thiolesterase 18; Ubp15; UBP18_HUMAN; USP18;

免疫原

免疫原:
Uniprot:

Q9UMW8(UBP18_HUMAN) >>Visit HPA database.

遺伝子(ID):
USP18(11274)
タンパク質配列:
MSKAFGLLRQICQSILAESSQSPADLEEKKEEDSNMKREQPRERPRAWDYPHGLVGLHNIGQTCCLNSLIQVFVMNVDFTRILKRITVPRGADEQRRSVPFQMLLLLEKMQDSRQKAVRPLELAYCLQKCNVPLFVQHDAAQLYLKLWNLIKDQITDVHLVERLQALYTIRVKDSLICVDCAMESSRNSSMLTLPLSLFDVDSKPLKTLEDALHCFFQPRELSSKSKCFCENCGKKTRGKQVLKLTHLPQTLTIHLMRFSIRNSQTRKICHSLYFPQSLDFSQILPMKRESCDAEEQSGGQYELFAVIAHVGMADSGHYCVYIRNAVDGKWFCFNDSNICLVSWEDIQCTYGNPNYHWQETAYLLVYMKMEC

PTMs - Q9UMW8 基板として

Site PTM Type Enzyme
K3 Ubiquitination
Y125 Phosphorylation
K173 Ubiquitination
K268 Ubiquitination
K288 Ubiquitination

研究背景

機能:

Involved in the regulation of inflammatory response to interferon type 1. Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.

Has enzymatic activity similar to isoform 1 and interferes with type I interferon signaling. Major deISGylation enzyme for nuclear proteins.

細胞の位置付け:

Cytoplasm.

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the peptidase C19 family.

参考文献

1). USP18‑deficiency in cervical carcinoma is crucial for the malignant behavior of tumor cells in an ERK signal‑dependent manner. Oncology Letters, 2021 (PubMed: 33850562) [IF=2.9]

Application: IHC    Species: Human    Sample: tumor tissues

Figure 1. Downregulated USP18 expression in tumor tissues of a cervix tissue microarray. UCC tissues with grade (A) 1 and (B) 2 exhibited moderate staining, and grade (C) 3 exhibited weak staining of USP18. (D) Non-cancer tissues exhibited super intensive staining of USP18. Magnification, ×200. USP18, ubiquitin-specific peptidase 18.

Application: WB    Species: Human    Sample: HeLa cells

Figure 2. USP18 expression in HeLa cells. (A) Representative images of USP18 protein expression from western blot assay. (B) Fluorescence images of mock- and USP18-siRNA-transfected HeLa cells. Magnification, ×200. (C) Statistical analysis of USP18 protein expression. **P<0.01 vs. si-USP18. USP18, ubiquitin-specific peptidase 18; siRNA, small interfering RNA.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.