MBD1 Antibody - #DF7981
| 製品: | MBD1 Antibody |
| カタログ: | DF7981 |
| タンパク質の説明: | Rabbit polyclonal antibody to MBD1 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Horse, Sheep, Rabbit, Dog |
| 分子量: | 67 kDa; 67kD(Calculated). |
| ユニプロット: | Q9UIS9 |
| RRID: | AB_2841368 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7981, RRID:AB_2841368.
折りたたみ／展開
CXXC 3; CXXC-type zinc finger protein 3; CXXC3; MBD 1; Mbd1; MBD1_HUMAN; MECP1 COMPLEX; Methyl CpG binding domain protein 1; Methyl CpG binding domain protein 1 isoform PCM1; Methyl CpG binding protein MBD1; Methyl CpG binding protein splice variant 1; Methyl CpG binding protein splice variant 2; Methyl CpG binding protein splice variant 3; Methyl CpG binding protein splice variant 4; Methyl-CpG-binding domain protein 1; Methyl-CpG-binding protein MBD1; PCM 1; PCM1; Protein containing methyl-CpG-binding domain 1; Regulator of fibroblast growth factor 2 (FGF 2) transcription; RFT; The regulator of fibroblast growth factor 2(FGF 2) transcription;
免疫原
- Q9UIS9 MBD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGILCYPAPKAHPVAVASKKRKKPSRPAKTRKRQVGPQSGEVRKEAPRDETKADTDTAPASFPAPGCCENCGISFSGDGTQRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECAACQVTEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTQEDCGHCPICLRPPRPGLRRQWKCVQRRCLRGKHARRKGGCDSKMAARRRPGAQPLPPPPPSQSPEPTEPHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGACAACLRRMDCGRCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWSESEDGAGSPPPYRRRKRPSSARRHHLGPTLKPTLATRTAQPDHTQAPTKQEAGGGFVLPPPGTDLVFLREGASSPVQVPGPVAASTEALLQEAQCSGLSWVVALPQVKQEKADTQDEWTPGTAVLTSPVLVPGCPSKAVDPGLPSVKQEPPDPEEDKEENKDDSASKLAPEEEAGGAGTPVITEIFSLGGTRFRDTAVWLPRSKDLKKPGARKQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UIS9 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S37 | Phosphorylation | Uniprot | |
| K46 | Sumoylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| Y52 | Phosphorylation | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| T116 | Phosphorylation | Uniprot | |
| T120 | Phosphorylation | Uniprot | |
| K277 | Sumoylation | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| T301 | Phosphorylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| S365 | Phosphorylation | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| S388 | Phosphorylation | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| S393 | Phosphorylation | Uniprot | |
| S399 | Phosphorylation | Uniprot | |
| Y403 | Phosphorylation | Uniprot | |
| K422 | Acetylation | Uniprot | |
| K422 | Sumoylation | Uniprot | |
| K422 | Ubiquitination | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| K499 | Sumoylation | Uniprot | |
| K502 | Sumoylation | Uniprot | |
| K502 | Ubiquitination | Uniprot | |
| T505 | Phosphorylation | Uniprot | |
| K538 | Sumoylation | Uniprot | |
| S557 | Phosphorylation | Uniprot | |
| K558 | Ubiquitination | Uniprot | |
| T570 | Phosphorylation | Uniprot | |
| T574 | Phosphorylation | Uniprot | |
| T587 | Phosphorylation | Uniprot | |
| R593 | Methylation | Uniprot | |
| K595 | Methylation | Uniprot |
研究背景
Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
Nucleus. Nucleus matrix. Nucleus speckle. Chromosome.
Note: Colocalizes with the Ten-1 ICD form of TENM1 in foci associated with the nuclear matrix (By similarity). Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.
Widely expressed.
Interacts with the Ten-1 ICD form of TENM1 (By similarity). Interacts with OASL, AFT7IP, AFT7IP2 and BAHD1. Binds CHAF1A and the SUV39H1-CBX5 complex via the MBD domain. Binds MGP via the TRD domain. May be part of the MeCP1 complex. During DNA replication, it recruits SETDB1 to form a S phase-specific complex that facilitates methylation of H3 'Lys-9' during replication-coupled chromatin assembly and is at least composed of the CAF-1 subunit CHAF1A, MBD1 and SETDB1.
The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
The third CXXC-type zinc finger mediates binding to DNA containing unmethylated CpG dinucleotides.
The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.