GRN Antibody - #DF7997

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製品: GRN Antibody
カタログ: DF7997
タンパク質の説明: Rabbit polyclonal antibody to GRN
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IHC
反応性: Human, Mouse, Rat, Monkey
予測: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 64 kDa; 64kD(Calculated).
ユニプロット: P28799
RRID: AB_2841380

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 100ul $280 在庫あり
 200ul $350 在庫あり

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MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
予測:
Bovine(100%), Horse(100%), Sheep(83%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
GRN Antibody detects endogenous levels of total GRN.
RRID:
AB_2841380
引用形式: Affinity Biosciences Cat# DF7997, RRID:AB_2841380.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Acrogranin; CLN11; GEP; GP88; Granulin A; Granulin B; Granulin C; Granulin D; Granulin E; Granulin epithelin; Granulin F; Granulin G; Granulin-7; Granulins; GRN; GRN_HUMAN; PC cell derived growth factor; PCDGF; PEPI; PGRN; Proepithelin; Progranulin;

免疫原

免疫原:

A synthesized peptide derived from human GRN, corresponding to a region within the internal amino acids.

Uniprot:

P28799(GRN_HUMAN) >>Visit HPA database.

遺伝子(ID):
GRN(2896)
発現特異性:
P28799 GRN_HUMAN:

In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.

タンパク質配列:
MWTLVSWVALTAGLVAGTRCPDGQFCPVACCLDPGGASYSCCRPLLDKWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQRSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGDVKCDMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTCVAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQPATFLARSPHVGVKDVECGEGHFCHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREAPRWDAPLRDPALRQLL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Rabbit
100
Sheep
83
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation. Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes. Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB. In addition, functions as wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures (By similarity). Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases. Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity.

Promotes proliferation of the epithelial cell line A431 in culture.

Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8.

Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity.

PTMs:

Cleaved by ELANE; proteolysis is blocked by SLPI and is concentration- and time-dependent and induces CXCL8/IL-8 production; granulin-3 and granulin-4 are resistant to ELANE. Cleaved by CTSL in lysosome thus regulating the maturation and turnover of progranulin within the lysosome.

細胞の位置付け:

Secreted. Lysosome.
Note: Endocytosed by SORT1 and delivred to lysosomes (PubMed:21092856, PubMed:28073925). Targeted to lysosome by PSAP via M6PR and LRP1, in both biosynthetic and endocytic pathways (PubMed:26370502, PubMed:28073925). Co-localized with GBA in the intracellular trafficking compartments until to lysosome (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.

タンパク質ファミリー:

Belongs to the granulin family.

参考文献

1). Identification of potential ferroptosis-associated biomarkers in rheumatoid arthritis. Frontiers in Immunology, 2023 (PubMed: 37492576) [IF=5.7]

Application: WB    Species: Human    Sample:

Figure 13 Validation of the hub genes in experimental samples. (A) Representative tracing of the PI and the joint swelling thickness in rat after CFA or Nor treatment in the two groups(n=12). (B) Representative images of H&E staining of joint sections from AIA rat or Nor rat. (C) The expression of GRN, PTGS2 and ENO1. n = 3. *p < 0.05, **p < 0.01, ***p < 0.001. (D) IHC staining for PTGS2, ENO1, and GRN on synovium from samples of OA and RA patients (n = 3).

Application: IHC    Species: Human    Sample:

Figure 13 Validation of the hub genes in experimental samples. (A) Representative tracing of the PI and the joint swelling thickness in rat after CFA or Nor treatment in the two groups(n=12). (B) Representative images of H&E staining of joint sections from AIA rat or Nor rat. (C) The expression of GRN, PTGS2 and ENO1. n = 3. *p < 0.05, **p < 0.01, ***p < 0.001. (D) IHC staining for PTGS2, ENO1, and GRN on synovium from samples of OA and RA patients (n = 3).
2). PGRN knockdown alleviates pulmonary fibrosis regulating the Akt/GSK3β signaling pathway. International immunopharmacology, 2025 (PubMed: 40088870) [IF=4.8]
3). Physical exercise mitigates the cognitive impairments by promoting ER-phagy in mice model of Alzheimer's disease. Behavioural brain research, 2025 (PubMed: 40414310) [IF=2.6]
4). Granulin in renal tubular epithelia is associated with interstitial inflammation and activates the TLR9-IFN-α pathway in lupus nephritis. Lupus, 2024 (PubMed: 38334360) [IF=1.9]

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