ACTN4 Antibody - #DF8000
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8000, RRID:AB_2841381.
折りたたみ／展開
actinin 4; Actinin alpha 4; actinin4; ACTN 4; ACTN4; ACTN4_HUMAN; Alpha-actinin-4; DKFZp686K23158; F actin cross linking protein; F-actin cross-linking protein; Focal segmental glomerulosclerosis 1; FSGS 1; FSGS; FSGS1; Non muscle alpha actinin 4; Non-muscle alpha-actinin 4;
免疫原
- O43707 ACTN4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDALGSLTHSRREALEKTEKQLEAIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGEAEFNRIMSLVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAVPGALDYKSFSTALYGESDL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O43707 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y4 | Phosphorylation | Q05397 (PTK2) | Uniprot |
| Y11 | Phosphorylation | Uniprot | |
| Y13 | Phosphorylation | Uniprot | |
| Y31 | Phosphorylation | Q05397 (PTK2) | Uniprot |
| K50 | Ubiquitination | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| T69 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| K114 | Acetylation | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| T142 | Phosphorylation | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| T163 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| Y212 | Phosphorylation | Uniprot | |
| K214 | Acetylation | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| K217 | Acetylation | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K233 | Acetylation | Uniprot | |
| K233 | Ubiquitination | Uniprot | |
| Y234 | Phosphorylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| T249 | Phosphorylation | Uniprot | |
| Y260 | Phosphorylation | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| Y265 | Phosphorylation | Q05397 (PTK2) | Uniprot |
| S269 | Phosphorylation | Uniprot | |
| R280 | Methylation | Uniprot | |
| K283 | Acetylation | Uniprot | |
| Y298 | Phosphorylation | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| R310 | Methylation | Uniprot | |
| T312 | Phosphorylation | Uniprot | |
| R319 | Methylation | Uniprot | |
| K323 | Ubiquitination | Uniprot | |
| T324 | Phosphorylation | Uniprot | |
| K331 | Acetylation | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| Y338 | Phosphorylation | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| C351 | S-Nitrosylation | Uniprot | |
| T359 | Phosphorylation | Uniprot | |
| T362 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| Y397 | Phosphorylation | Uniprot | |
| K417 | Acetylation | Uniprot | |
| K417 | Ubiquitination | Uniprot | |
| K421 | Ubiquitination | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| T429 | Phosphorylation | Uniprot | |
| K432 | Acetylation | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| K437 | Acetylation | Uniprot | |
| Y441 | Phosphorylation | Uniprot | |
| T445 | Phosphorylation | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| K450 | Ubiquitination | Uniprot | |
| K455 | Acetylation | Uniprot | |
| K455 | Ubiquitination | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| R469 | Methylation | Uniprot | |
| Y485 | Phosphorylation | Uniprot | |
| Y486 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| T493 | Phosphorylation | Uniprot | |
| K497 | Ubiquitination | Uniprot | |
| C499 | S-Nitrosylation | Uniprot | |
| S507 | Phosphorylation | Uniprot | |
| T509 | Phosphorylation | Uniprot | |
| S511 | Phosphorylation | Uniprot | |
| K521 | Ubiquitination | Uniprot | |
| Y533 | Phosphorylation | Uniprot | |
| K592 | Acetylation | Uniprot | |
| K592 | Ubiquitination | Uniprot | |
| K604 | Ubiquitination | Uniprot | |
| S608 | Phosphorylation | Uniprot | |
| Y611 | Phosphorylation | Uniprot | |
| T612 | Phosphorylation | Uniprot | |
| T615 | Phosphorylation | Uniprot | |
| S621 | Phosphorylation | Uniprot | |
| K622 | Acetylation | Uniprot | |
| K622 | Ubiquitination | Uniprot | |
| K625 | Acetylation | Uniprot | |
| K625 | Ubiquitination | Uniprot | |
| K632 | Ubiquitination | Uniprot | |
| S642 | Phosphorylation | Uniprot | |
| K643 | Ubiquitination | Uniprot | |
| S656 | Phosphorylation | Uniprot | |
| T667 | Phosphorylation | Uniprot | |
| T682 | Phosphorylation | Uniprot | |
| Y693 | Phosphorylation | Uniprot | |
| S696 | Phosphorylation | Uniprot | |
| Y700 | Phosphorylation | Uniprot | |
| K701 | Ubiquitination | Uniprot | |
| Y727 | Phosphorylation | Uniprot | |
| T741 | Phosphorylation | Uniprot | |
| T746 | Phosphorylation | Uniprot | |
| T756 | Phosphorylation | Uniprot | |
| K760 | Ubiquitination | Uniprot | |
| S763 | Phosphorylation | Uniprot | |
| S773 | Phosphorylation | Uniprot | |
| K779 | Acetylation | Uniprot | |
| K779 | Methylation | Uniprot | |
| K779 | Ubiquitination | Uniprot | |
| C793 | S-Nitrosylation | Uniprot | |
| S796 | Phosphorylation | Uniprot | |
| R805 | Methylation | Uniprot | |
| T839 | Phosphorylation | Uniprot | |
| T840 | Phosphorylation | Uniprot | |
| T842 | Phosphorylation | Uniprot | |
| T844 | Phosphorylation | Uniprot | |
| K853 | Acetylation | Uniprot | |
| K859 | Acetylation | Uniprot | |
| K859 | Ubiquitination | Uniprot | |
| R868 | Methylation | Uniprot | |
| Y878 | Phosphorylation | Uniprot | |
| Y898 | Phosphorylation | Uniprot | |
| K899 | Acetylation | Uniprot | |
| Y906 | Phosphorylation | Uniprot | |
| S909 | Phosphorylation | Uniprot |
研究背景
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA.
Nucleus. Cytoplasm. Cell junction. Cytoplasm>Cytoskeleton>Stress fiber.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines (breast cancer cell line MCF-7, oral floor cancer IMC-2, and bladder cancer KU-7).
Widely expressed.
Homodimer; antiparallel (By similarity). Binds TRIM3 at the N-terminus (By similarity). Interacts with MICALL2 (preferentially in opened conformation); stimulated by RAB13 activation (By similarity). Identified in a complex with CASK, IQGAP1, MAGI2, NPHS1, SPTAN1 and SPTBN1 (By similarity). Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Interacts with BAIAP1 and PDLIM2. Interacts with PPARG and RARA. Binds to VCL; this interaction triggers VCL conformational changes.
Contains one Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif that mediates interaction with nuclear receptors.
Belongs to the alpha-actinin family.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Human Diseases > Immune diseases > Systemic lupus erythematosus.
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
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