UBE2L3 Antibody - #DF8031
| 製品: | UBE2L3 Antibody |
| カタログ: | DF8031 |
| タンパク質の説明: | Rabbit polyclonal antibody to UBE2L3 |
| アプリケーション: | WB |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 18 kDa; 18kD(Calculated). |
| ユニプロット: | P68036 |
| RRID: | AB_2841399 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8031, RRID:AB_2841399.
折りたたみ／展開
E2 F1; L UBC; L-UBC; UB2L3_HUMAN; UBCE7; UbcH7; UbcM4; Ube2l3; Ubiquitin carrier protein L3; Ubiquitin conjugating enzyme E2 L3; Ubiquitin protein ligase L3; Ubiquitin-conjugating enzyme E2 L3; Ubiquitin-conjugating enzyme E2-F1; Ubiquitin-protein ligase L3;
免疫原
A synthesized peptide derived from human UBE2L3, corresponding to a region within C-terminal amino acids.
- P68036 UB2L3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
研究背景
Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation. Autoubiquitinated in vitro.
Nucleus. Cytoplasm.
Ubiquitous, with highest expression in testis.
In contrast to other ubiquitin-conjugating enzymes E2, residues essential for lysine reactivity are absent: Pro and a His residues are present instead of an Asp and an Asp residues in positions 88 and 119, respectively.
Belongs to the ubiquitin-conjugating enzyme family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
· Human Diseases > Neurodegenerative diseases > Parkinson's disease.
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