製品: HSPA14 Antibody
カタログ: DF8060
タンパク質の説明: Rabbit polyclonal antibody to HSPA14
アプリケーション: WB IF/ICC
反応性: Human, Monkey
予測: Pig, Bovine, Horse, Rabbit, Dog
分子量: 55 kDa; 55kD(Calculated).
ユニプロット: Q0VDF9
RRID: AB_2841416

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Monkey
予測:
Pig(100%), Bovine(80%), Horse(80%), Rabbit(91%), Dog(90%)
クローナリティ:
Polyclonal
特異性:
HSPA14 Antibody detects endogenous levels of total HSPA14.
RRID:
AB_2841416
引用形式: Affinity Biosciences Cat# DF8060, RRID:AB_2841416.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Heat shock 70 kDa protein 14; Heat shock 70kDa protein 14; Heat shock protein HSP60; Heat shock protein hsp70 related protein; HSP60; HSP70 4; HSP70 like protein 1; HSP70-like protein 1; HSP70L1; HSP7E_HUMAN; HSPA 14; HSPA14; MGC131990;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MAAIGVHLGCTSACVAVYKDGRAGVVANDAGDRVTPAVVAYSENEEIVGLAAKQSRIRNISNTVMKVKQILGRSSSDPQAQKYIAESKCLVIEKNGKLRYEIDTGEETKFVNPEDVARLIFSKMKETAHSVLGSDANDVVITVPFDFGEKQKNALGEAARAAGFNVLRLIHEPSAALLAYGIGQDSPTGKSNILVFKLGGTSLSLSVMEVNSGIYRVLSTNTDDNIGGAHFTETLAQYLASEFQRSFKHDVRGNARAMMKLTNSAEVAKHSLSTLGSANCFLDSLYEGQDFDCNVSRARFELLCSPLFNKCIEAIRGLLDQNGFTADDINKVVLCGGSSRIPKLQQLIKDLFPAVELLNSIPPDEVIPIGAAIEAGILIGKENLLVEDSLMIECSARDILVKGVDESGASRFTVLFPSGTPLPARRQHTLQAPGSISSVCLELYESDGKNSAKEETKFAQVVLQDLDKKENGLRDILAVLTMKRDGSLHVTCTDQETGKCEAISIEIAS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Rabbit
91
Dog
90
Horse
80
Bovine
80
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q0VDF9 基板として

Site PTM Type Enzyme
K53 Ubiquitination
K66 Ubiquitination
K68 Ubiquitination
S75 Phosphorylation
S76 Phosphorylation
K82 Ubiquitination
S87 Phosphorylation
K88 Ubiquitination
Y100 Phosphorylation
K109 Ubiquitination
K123 Ubiquitination
K125 Ubiquitination
K150 Ubiquitination
S186 Phosphorylation
S219 Phosphorylation
K260 Acetylation
Y286 Phosphorylation
S305 Phosphorylation
K310 Ubiquitination
K331 Ubiquitination
K343 Ubiquitination
K402 Ubiquitination
T420 Phosphorylation
T456 Phosphorylation
K457 Ubiquitination
K468 Ubiquitination
K469 Acetylation
T481 Phosphorylation
S487 Phosphorylation

研究背景

機能:

Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity.

細胞の位置付け:

Cytoplasm>Cytosol.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Component of ribosome-associated complex (RAC), a heterodimer composed of Hsp70/DnaK-type chaperone HSPA14 and Hsp40/DnaJ-type chaperone DNAJC2.

タンパク質ファミリー:

Belongs to the heat shock protein 70 family.

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