ABI2 Antibody - #DF8070
| 製品: | ABI2 Antibody |
| カタログ: | DF8070 |
| タンパク質の説明: | Rabbit polyclonal antibody to ABI2 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 80 kDa; 56kD(Calculated). |
| ユニプロット: | Q9NYB9 |
| RRID: | AB_2841425 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8070, RRID:AB_2841425.
折りたたみ／展開
Abelson interactor 2; ABI 2; Abi-2; ABI2; ABI2_HUMAN; ABI2B; Abl binding protein 3; Abl interacting protein 1 (SH3 containing protein); Abl interactor 2; Abl interactor protein 2b; Abl-binding protein 3; AblBP3; AIP 1; Arg binding protein 1; Arg protein tyrosine kinase binding protein; Arg-binding protein 1; ArgBP1; ARGBPIA; ArgBPIB; OTTHUMP00000163783; OTTHUMP00000163784; OTTHUMP00000206182; OTTHUMP00000206183; OTTHUMP00000206184; OTTHUMP00000206185; SSH3BP2;
免疫原
Widely expressed. Abundant in testes, ovary, thymus, and colon, with lower but detectable levels in prostate, peripheral blood leukocytes, and spleen.
- Q9NYB9 ABI2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAELQMLLEEEIPGGRRALFDSYTNLERVADYCENNYIQSADKQRALEETKAYTTQSLASVAYLINTLANNVLQMLDIQASQLRRMESSINHISQTVDIHKEKVARREIGILTTNKNTSRTHKIIAPANLERPVRYIRKPIDYTILDDIGHGVKWLLRFKVSTQNMKMGGLPRTTPPTQKPPSPPMSGKGTLGRHSPYRTLEPVRPPVVPNDYVPSPTRNMAPSQQSPVRTASVNQRNRTYSSSGSSGGSHPSSRSSSRENSGSGSVGVPIAVPTPSPPSVFPAPAGSAGTPPLPATSASAPAPLVPATVPSSTAPNAAAGGAPNLADGFTSPTPPVVSSTPPTGHPVQFYSMNRPASRHTPPTIGGSLPYRRPPSITSQTSLQNQMNGGPFYSQNPVSDTPPPPPPVEEPVFDESPPPPPPPEDYEEEEAAVVEYSDPYAEEDPPWAPRSYLEKVVAIYDYTKDKEDELSFQEGAIIYVIKKNDDGWYEGVMNGVTGLFPGNYVESIMHYSE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NYB9 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K43 | Ubiquitination | Uniprot | |
| S88 | Phosphorylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| T174 | Phosphorylation | Uniprot | |
| T175 | Phosphorylation | Uniprot | |
| T178 | Phosphorylation | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| T191 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| Y198 | Phosphorylation | Uniprot | |
| T200 | Phosphorylation | Uniprot | |
| R205 | Methylation | Uniprot | |
| Y213 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| T218 | Phosphorylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| R230 | Methylation | Uniprot | |
| T231 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| T240 | Phosphorylation | Uniprot | |
| Y241 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| T361 | Phosphorylation | Uniprot | |
| T364 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| Y371 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| Y460 | Phosphorylation | Uniprot | |
| T463 | Phosphorylation | Uniprot | |
| K466 | Acetylation | Uniprot | |
| K482 | Acetylation | Uniprot | |
| S512 | Phosphorylation | Uniprot |
研究背景
Regulator of actin cytoskeleton dynamics underlying cell motility and adhesion. Functions as a component of the WAVE complex, which activates actin nucleating machinery Arp2/3 to drive lamellipodia formation. Acts as regulator and substrate of nonreceptor tyrosine kinases ABL1 and ABL2 involved in processes linked to cell growth and differentiation. Positively regulates ABL1-mediated phosphorylation of ENAH, which is required for proper polymerization of nucleated actin filaments at the leading edge. Contributes to the regulation of actin assembly at the tips of neuron projections. In particular, controls dendritic spine morphogenesis and may promote dendritic spine specification toward large mushroom-type spines known as repositories of memory in the brain (By similarity). In hippocampal neurons, may mediate actin-dependent BDNF-NTRK2 early endocytic trafficking that triggers dendrite outgrowth (By similarity). Participates in ocular lens morphogenesis, likely by regulating lamellipodia-driven adherens junction formation at the epithelial cell-secondary lens fiber interface (By similarity). Also required for nascent adherens junction assembly in epithelial cells.
Phosphorylated by ABL1.
Cytoplasm. Nucleus.
Cell projection>Lamellipodium. Cell projection>Filopodium. Cytoplasm>Cytoskeleton. Cell junction>Adherens junction.
Note: Isoform 1 but not isoform 3 is localized to protruding lamellipodia and filopodia tips (PubMed:11516653, PubMed:15572692). Present at nascent adherens junctions, where it clusters adjacent to the tips of F-actin protrusions (PubMed:15572692).
Widely expressed. Abundant in testes, ovary, thymus, and colon, with lower but detectable levels in prostate, peripheral blood leukocytes, and spleen.
Component of the WAVE complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. Interacts (via SH3 domain) with ABL1 and ABL2.
(Microbial infection) Interacts with human cytomegalovirus UL135.
The SH3 domain is critical for binding to ABL1 and ABL2.
Belongs to the ABI family.
研究領域
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.