TSLP Antibody - #DF8077

(1)   (2)  
製品: TSLP Antibody
カタログ: DF8077
タンパク質の説明: Rabbit polyclonal antibody to TSLP
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Horse
分子量: 18 kDa; 18kD(Calculated).
ユニプロット: Q969D9
RRID: AB_2841431

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Horse(80%)
クローナリティ:
Polyclonal
特異性:
TSLP Antibody detects endogenous levels of total TSLP.
RRID:
AB_2841431
引用形式: Affinity Biosciences Cat# DF8077, RRID:AB_2841431.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Thymic stromal lymphopoietin; Thymic stromal lymphopoietin protein TSLP; Tslp; TSLP protein; TSLP_HUMAN;

免疫原

免疫原:
Uniprot:

Q969D9(TSLP_HUMAN) >>Visit HPA database.

遺伝子(ID):
TSLP(85480)
発現特異性:
Q969D9 TSLP_HUMAN:

Isoform 1 is expressed in a number of tissues including heart, liver and prostate. Isoform 2 is the predominant form in keratinocytes of oral mucosa, skin and in salivary glands. It is secreted into saliva.

タンパク質配列:
MFPFALLYVLSVSFRKIFILQLVGLVLTYDFTNCDFEKIKAAYLSTISKDLITYMSGTKSTEFNNTVSCSNRPHCLTEIQSLTFNPTAGCASLAKEMFAMKTKAALAIWCPGYSETQINATQAMKKRRKRKVTTNKCLEQVSQLQGLWRRFNRPLLKQQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
80
Dog
70
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.

May act as an antimicrobial peptide in the oral cavity and on the skin.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Isoform 1 is expressed in a number of tissues including heart, liver and prostate. Isoform 2 is the predominant form in keratinocytes of oral mucosa, skin and in salivary glands. It is secreted into saliva.

サブユニット構造:

Interacts with a receptor composed of CRLF2 and IL7R. Binding of TSLP to CRLF2/TSLPR is a mechanistic prerequisite for recruitment of IL7R to the high-affinity ternary complex.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

参考文献

1). RBM15-mediated N6-methyladenosine modification affects COVID-19 severity by regulating the expression of multitarget genes. Cell death & disease, 2021 (PubMed: 34301919) [IF=9.0]

Application: WB    Species: Human    Sample:

Fig. 2 Aberrant methylation triggered by SARS-CoV-2 regulates multiple targets related to immune response and cell death pathways. Heatmap of genes (P 1.5) related to the cell death (A) and immune response (B). Box plot (C) depicting the expression levels of CASP1, CASP5, DDX3X, TRIB1, IL17RB, and TLSP in patients with COVID-19 (20 severe and 19 mild patients) as compared with those in healthy controls (n = 20). And scatter plots revealed m6A enrichment of CASP1, CASP5, DDX3X, TRIB1, IL17RB, and TSLP in patients. D Total m6A levels of COVID-19 patients and healthy controls (n = 3). E The protein levels of candidate genes were detected by western blot in COVID-19 patients and healthy controls (HCs). F CASP1, CASP5, DDX3X, TRIB1, IL17RB, and TLSP expression levels in HuT 78 cells co-cultured with activated THP-1 cells were measured after being treated with S proteins separately for 0, 6, 12, and 24 h. HuT 78 cells were co-cultured with (Ctrl) or without (spike) spike proteins (50 ng/ml), and cell death was detected by apoptosis assay (G), quantitative analysis of positive signals was showed in (H), and the protein levels of cleaved–CASP3 were detected using western blot (I). CCK-8 assay (J) was applied to evaluate proliferation abilities of HuT 78 cells with spike stimulated or without spike. Data were shown as means ± SD (n = 3, *P 

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