TRIP10 Antibody - #DF8087
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8087, RRID:AB_2841438.
折りたたみ／展開
Cdc42 interacting protein 4; Cdc42 interaction protein 4 long isoform; Cdc42-interacting protein 4; CG11341; CG15015 PA; Cip 4; CIP4; CIP4_HUMAN; DCIP4; hSTP; Protein Felic; Salt tolerant protein; Salt tolerator; STOT; STP; Thyroid hormone receptor interactor 10; Thyroid receptor interacting protein 10; Thyroid receptor-interacting protein 10; TR-interacting protein 10; TRIP 10; TRIP-10; trip10; Truncated Cdc42 interaction protein 4;
免疫原
Expressed in brain, colon, heart, kidney, liver, lung, megakaryocyte, ovary, pancreas, peripheral blood lymphocytes, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus and trachea.
- Q15642 CIP4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDWGTELWDQFEVLERHTQWGLDLLDRYVKFVKERTEVEQAYAKQLRSLVKKYLPKRPAKDDPESKFSQQQSFVQILQEVNDFAGQRELVAENLSVRVCLELTKYSQEMKQERKMHFQEGRRAQQQLENGFKQLENSKRKFERDCREAEKAAQTAERLDQDINATKADVEKAKQQAHLRSHMAEESKNEYAAQLQRFNRDQAHFYFSQMPQIFDKLQDMDERRATRLGAGYGLLSEAELEVVPIIAKCLEGMKVAANAVDPKNDSHVLIELHKSGFARPGDVEFEDFSQPMNRAPSDSSLGTPSDGRPELRGPGRSRTKRWPFGKKNKPRPPPLSPLGGPVPSALPNGPPSPRSGRDPLAILSEISKSVKPRLASFRSLRGSRGTVVTEDFSHLPPEQQRKRLQQQLEERSRELQKEVDQREALKKMKDVYEKTPQMGDPASLEPQIAETLSNIERLKLEVQKYEAWLAEAESRVLSNRGDSLSRHARPPDPPASAPPDSSSNSASQDTKESSEEPPSEESQDTPIYTEFDEDFEEEPTSPIGHCVAIYHFEGSSEGTISMAEGEDLSLMEEDKGDGWTRVRRKEGGEGYVPTSYLRVTLN
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15642 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R27 | Methylation | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| K104 | Acetylation | Uniprot | |
| K132 | Acetylation | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| Y190 | Phosphorylation | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| T302 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| S442 | Phosphorylation | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| S477 | Phosphorylation | Uniprot | |
| S482 | Phosphorylation | Uniprot | |
| T579 | Phosphorylation | Uniprot | |
| Y590 | Phosphorylation | Uniprot |
研究背景
Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL.
Tyrosine phosphorylated. Also phosphorylated by PKA.
Cytoplasm>Cytoskeleton. Cytoplasm>Cell cortex. Lysosome. Golgi apparatus. Cell membrane. Cell projection>Phagocytic cup.
Note: Translocates to the plasma membrane in response to insulin stimulation, and this may require active RHOQ (By similarity). Localizes to cortical regions coincident with F-actin, to lysosomes and to sites of phagocytosis in macrophages. Also localizes to the Golgi, and this requires AKAP9.
Cytoplasm>Perinuclear region.
Expressed in brain, colon, heart, kidney, liver, lung, megakaryocyte, ovary, pancreas, peripheral blood lymphocytes, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus and trachea.
Interacts specifically with GTP-bound RHOQ. Interacts with DNM2 and PDE6G (By similarity). Homodimerizes, the dimers can polymerize end-to-end to form filamentous structures. Interacts specifically with GTP-bound CDC42. Interacts with AKAP9, ARHGAP17, DAAM1, DIAPH1, DIAPH2, DNM1, FASLG/FASL, GAPVD1, LYN, microtubules, SRC, WAS/WASP and WASL/N-WASP. Interacts with the ligand binding domain of the thyroid receptor (TR) in the presence of thyroid hormone. May interact with CTNNB1 and HD/HTT.
The F-BAR domain binds the phospholipid membrane with its concave surface. The end-to-end polymerization of dimers of these domains provides a curved surface that fits best membranes with around 600 A diameter, and may drive tubulation.
Belongs to the FNBP1 family.
研究領域
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
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