EVL Antibody - #DF8091
| 製品: | EVL Antibody |
| カタログ: | DF8091 |
| タンパク質の説明: | Rabbit polyclonal antibody to EVL |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Dog |
| 分子量: | 50 kDa; 45kD(Calculated). |
| ユニプロット: | Q9UI08 |
| RRID: | AB_2841440 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8091, RRID:AB_2841440.
折りたたみ／展開
AI528774; Ena-vasodilator stimulated phosphoprotein; Ena/vasodilator stimulated phosphoprotein like; Ena/VASP like protein; EVL; RNB 6; RNB6;
免疫原
- Q9UI08 EVL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSEQSICQARASVMVYDDTSKKWVPIKPGQQGFSRINIYHNTASNTFRVVGVKLQDQQVVINYSIVKGLKYNQATPTFHQWRDARQVYGLNFASKEEATTFSNAMLFALNIMNSQEGGPSSQRQVQNGPSPDEMDIQRRQVMEQHQQQRQESLERRTSATGPILPPGHPSSAASAPVSCSGPPPPPPPPVPPPPTGATPPPPPPLPAGGAQGSSHDESSMSGLAAAIAGAKLRRVQRPEDASGGSSPSGTSKSDANRASSGGGGGGLMEEMNKLLAKRRKAASQSDKPAEKKEDESQMEDPSTSPSPGTRAASQPPNSSEAGRKPWERSNSVEKPVSSILSRTPSVAKSPEAKSPLQSQPHSRMKPAGSVNDMALDAFDLDRMKQEILEEVVRELHKVKEEIIDAIRQELSGISTT
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UI08 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y16 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| Y39 | Phosphorylation | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| T250 | Phosphorylation | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| T303 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| S331 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| T343 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| K365 | Ubiquitination | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| K399 | Ubiquitination | Uniprot |
研究背景
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization.
Phosphorylated by PKA; phosphorylation abolishes binding to SH3 domains of ABL and SRC.
Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Stress fiber. Cell projection>Lamellipodium.
Note: Targeted to the leading edge of lamellipodia and the distal tip of stress fibers through interaction with a number of proteins. In activated T-cells, localizes to the F-actin collar and the distal tip of microspikes.
Homotetramer (By similarity). Binds to the SH3 domains of ABL1, LYN and SRC. Also binds to profilin, with preference for isoform IIa of PFN2, and the WW domain of APBB1/FE65. Binds to SEMA6A. Interacts, via the Pro-rich region, with the C-terminal SH3 domain of DNMBP. Interacts with RAPH1. Binds, via the EVH1 domain, the Pro-rich domain of Listeria monocytogenes actA (By similarity). Binds, via the EVH1 domain, the Pro-rich domain of ZYX. Interacts with FYB1. Interacts with ZDHHC17.
The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
Belongs to the Ena/VASP family.
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