Mitofusin 2 Antibody - #DF8106

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製品: Mitofusin 2 Antibody
カタログ: DF8106
タンパク質の説明: Rabbit polyclonal antibody to Mitofusin 2
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 86 kDa; 86kD(Calculated).
ユニプロット: O95140
RRID: AB_2841449

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(87%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%), Xenopus(86%)
クローナリティ:
Polyclonal
特異性:
Mitofusin 2 Antibody detects endogenous levels of total Mitofusin 2.
RRID:
AB_2841449
引用形式: Affinity Biosciences Cat# DF8106, RRID:AB_2841449.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CMT2A; CMT2A2; CPRP 1; CPRP1; EC 3.6.5.-; Fzo; HSG; hyperplasia suppressor gene; Hypertension related protein 1; KIAA0214; MARF; MFN 2; Mfn2; MFN2_HUMAN; Mitochondrial assembly regulatory factor; Mitofusin-2; Mitofusin2; Transmembrane GTPase MFN2;

免疫原

免疫原:
Uniprot:

O95140(MFN2_HUMAN) >>Visit HPA database.

遺伝子(ID):
MFN2(9927)
発現特異性:
O95140 MFN2_HUMAN:

Ubiquitous; expressed at low level. Highly expressed in heart and kidney.

タンパク質配列:
MSLLFSRCNSIVTVKKNKRHMAEVNASPLKHFVTAKKKINGIFEQLGAYIQESATFLEDTYRNAELDPVTTEEQVLDVKGYLSKVRGISEVLARRHMKVAFFGRTSNGKSTVINAMLWDKVLPSGIGHTTNCFLRVEGTDGHEAFLLTEGSEEKRSAKTVNQLAHALHQDKQLHAGSLVSVMWPNSKCPLLKDDLVLMDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKHFFHKVSERLSRPNIFILNNRWDASASEPEYMEEVRRQHMERCTSFLVDELGVVDRSQAGDRIFFVSAKEVLNARIQKAQGMPEGGGALAEGFQVRMFEFQNFERRFEECISQSAVKTKFEQHTVRAKQIAEAVRLIMDSLHMAAREQQVYCEEMREERQDRLKFIDKQLELLAQDYKLRIKQITEEVERQVSTAMAEEIRRLSVLVDDYQMDFHPSPVVLKVYKNELHRHIEEGLGRNMSDRCSTAITNSLQTMQQDMIDGLKPLLPVSVRSQIDMLVPRQCFSLNYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRALMGYNDQVQRPIPLTPANPSMPPLPQGSLTQEEFMVSMVTGLASLTSRTSMGILVVGGVVWKAVGWRLIALSFGLYGLLYVYERLTWTTKAKERAFKRQFVEHASEKLQLVISYTGSNCSHQVQQELSGTFAHLCQQVDVTRENLEQEIAAMNKKIEVLDSLQSKAKLLRNKAGWLDSELNMFTHQYLQPSR

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
87
Xenopus
86
Chicken
80
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95140 基板として

Site PTM Type Enzyme
Ubiquitination
S10 Phosphorylation
S27 Phosphorylation P45984 (MAPK9)
K30 Ubiquitination
K36 Acetylation
K36 Ubiquitination
K79 Ubiquitination
K84 Ubiquitination
K98 Ubiquitination
T111 Phosphorylation
K154 Ubiquitination
K158 Ubiquitination
K171 Ubiquitination
K192 Ubiquitination
K243 Acetylation
K243 Ubiquitination
K307 Ubiquitination
K316 Ubiquitination
K355 Acetylation
K355 Ubiquitination
K357 Ubiquitination
K366 Ubiquitination
K406 Ubiquitination
K416 Ubiquitination
K420 Ubiquitination
T423 Phosphorylation
S442 Phosphorylation
K460 Ubiquitination
K463 Ubiquitination
K560 Ubiquitination
K662 Ubiquitination
K719 Ubiquitination
K720 Ubiquitination
K730 Ubiquitination
K737 Ubiquitination
S756 Phosphorylation

研究背景

機能:

Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains (Probable). Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes (By similarity). Plays an important role in the regulation of vascular smooth muscle cell proliferation (By similarity). Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PRKN recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress (By similarity). Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions (By similarity).

PTMs:

Phosphorylated by PINK1.

Ubiquitinated by non-degradative ubiquitin by PRKN, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion.

細胞の位置付け:

Mitochondrion outer membrane>Multi-pass membrane protein.
Note: Colocalizes with BAX during apoptosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous; expressed at low level. Highly expressed in heart and kidney.

サブユニット構造:

Forms homomultimers and heteromultimers with MFN1. Oligomerization is essential for mitochondrion fusion (Probable). Interacts with VAT1 (By similarity). Interacts with STOML2; may form heterooligomers. Interacts (phosphorylated) with PRKN. Interacts with EIF2AK3 (By similarity).

タンパク質ファミリー:

A helix bundle is formed by helices from the N-terminal and the C-terminal part of the protein. The GTPase domain cannot be expressed by itself, without the helix bundle. Rearrangement of the helix bundle and/or of the coiled coil domains may bring membranes from adjacent mitochondria into close contact, and thereby play a role in mitochondrial fusion.

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.

研究領域

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

参考文献

1). Ginsenoside CK improves skeletal muscle insulin resistance by activating DRP1/PINK1-mediated mitophagy. Food & Function, 2023 (PubMed: 36562271) [IF=6.1]
2). FGFR2 Mutation p.Cys342Arg Enhances Mitochondrial Metabolism-Mediated Osteogenesis via FGF/FGFR-AMPK-Erk1/2 Axis in Crouzon Syndrome. Cells, 2022 (PubMed: 36231091) [IF=6.0]

Application: WB    Species: Human    Sample:

Figure 7 Mitochondrial dynamics participate in osteogenesis mediated by FGFR/FGFR2-AMPK pathway: (A) Mito-tracker red and mitochondria 2D analysis showed more fragmented or punctate mitochondria with fewer and shorter branches in the MT group, while there were widely reticular mitochondria with longer branches in the WT group. (B) qRT-PCR and Western blot analysis demonstrated that the expressions of mitochondrial-fusion-related factors Mfn2 and Opa1 were downregulated, however, the expression of mitochondrial-fission-related factor Drp1 was upregulated in the MT group compared to the WT group. (C) Mito-tracker red and mitochondria 2D analysis showed a filamentous network of mitochondria with more and longer branches widely spread in the cytoplasm in the siFGFR2 group. (D) qPCR and Western blot analysis showed that the expression of fusion-related genes Mfn2 and Opa1 was increased and the expression of fission-related gene Drp1 was decreased in the siFGFR2 group. (E) After treatment with Compound C, the morphology of the mitochondria which should have tended to split adopted a fused reticular structure with more and longer branches, as shown by Mito-tracker red and mitochondria 2D analysis. (F) Western blot analysis showed the level of MFN2 and OPA1 was increased and then level of DRP1 was decreased, as induced by Compound C. p values were significant at * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.
3). Targeted inhibition of CX3CL1 limits podocytes ferroptosis to ameliorate cisplatin-induced acute kidney injury. Molecular medicine (Cambridge, Mass.), 2023 (PubMed: 37875838) [IF=5.7]

Application: WB    Species: Mouse    Sample: kidney tissues

Fig. 4 The mitochondrial function was improved by CX3CL1 deficiency in mice with cisplatin-induced AKI. A TEM images of renal tissues were captured. Scale bar = 500 nm. B The relative expression profiles of the mitochondrial proteins UCP2, Mfn2, and PGC1α in kidney tissues were determined by Western blotting. C DHE staining was conducted to determine the ROS level in renal tissues. Scale bar = 20 μm. D TEM was employed to capture images of podocytes. Scale bar = 500 nm. E Western blot analysis illustrating the relative expression patterns of the mitochondrial proteins UCP2, Mfn2, and PGC1α in podocytes. F The ROS level was detected through DCFH-DA labeling. Scale bar = 10 μm. G JC-1 staining was conducted to detect MMP in podocytes. Scale bar = 10 μm. (AKI, acute kidney injury; TEM, transmission electron microscopy; UCP2, uncoupling protein 2; Mfn2, Mitofusin 2; PGC1α, peroxisome proliferators-activated receptor γ coactivator l-alpha; DHE, dihydroethidium; ROS, reactive oxygen species; DCFH-DA, 2,7-dichlorodihydrofluorescein diacetate; MMP, matrix metalloproteinase; P value was calculated by one-way analysis of variance and Tukey’s test.
4). Patchouli alcohol alleviates metabolic dysfunction-associated steatohepatitis via inhibiting mitochondria-associated endoplasmic reticulum membrane disruption-induced hepatic steatosis and inflammation in rats. International immunopharmacology, 2024 (PubMed: 38971107) [IF=5.6]
5). ASA VI controls osteoarthritis in mice by maintaining mitochondrial homeostasis through Sirtuin 3. International immunopharmacology, 2024 (PubMed: 39111145) [IF=5.6]
6). Effect and molecular mechanism of Sulforaphane alleviates brain damage caused by acute carbon monoxide poisoning:Network pharmacology analysis, molecular docking, and experimental evidence. Environmental toxicology, 2024 (PubMed: 37860845) [IF=4.5]
7). GSDMD induces hepatocyte pyroptosis to trigger alcoholic hepatitis through modulating mitochondrial dysfunction. Cell division, 2024 (PubMed: 38532477) [IF=2.3]
8). Berberine protects against palmitate induced beta cell injury via promoting mitophagy. Genes & genomics, 2022 (PubMed: 35633490) [IF=2.1]
9). Melatonin Alleviates Acute Kidney Injury by Inhibiting NRF2/Slc7a11 Axis-Mediated Ferroptosis. Oxidative Medicine and Cellular Longevity, 2022 (PubMed: 35979396)

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