XDH Antibody - #DF8111

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製品: XDH Antibody
カタログ: DF8111
タンパク質の説明: Rabbit polyclonal antibody to XDH
アプリケーション: WB IHC
反応性: Human, Rat
予測: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
分子量: 146 kDa; 146kD(Calculated).
ユニプロット: P47989
RRID: AB_2841454

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
予測:
Pig(88%), Bovine(100%), Sheep(88%), Rabbit(100%), Dog(100%), Chicken(88%)
クローナリティ:
Polyclonal
特異性:
XDH Antibody detects endogenous levels of total XDH.
RRID:
AB_2841454
引用形式: Affinity Biosciences Cat# DF8111, RRID:AB_2841454.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Xanthine dehydrogenase; Xanthine dehydrogenase/oxidase; Xanthine oxidase; Xanthine oxidoreductase; XD; XDH; XDH_HUMAN; xdha; XO; xor;

免疫原

免疫原:
Uniprot:

P47989(XDH_HUMAN) >>Visit HPA database.

遺伝子(ID):
XDH(7498)
発現特異性:
P47989 XDH_HUMAN:

Detected in milk (at protein level).

タンパク質配列:
MTADKLVFFVNGRKVVEKNADPETTLLAYLRRKLGLSGTKLGCGEGGCGACTVMLSKYDRLQNKIVHFSANACLAPICSLHHVAVTTVEGIGSTKTRLHPVQERIAKSHGSQCGFCTPGIVMSMYTLLRNQPEPTMEEIENAFQGNLCRCTGYRPILQGFRTFARDGGCCGGDGNNPNCCMNQKKDHSVSLSPSLFKPEEFTPLDPTQEPIFPPELLRLKDTPRKQLRFEGERVTWIQASTLKELLDLKAQHPDAKLVVGNTEIGIEMKFKNMLFPMIVCPAWIPELNSVEHGPDGISFGAACPLSIVEKTLVDAVAKLPAQKTEVFRGVLEQLRWFAGKQVKSVASVGGNIITASPISDLNPVFMASGAKLTLVSRGTRRTVQMDHTFFPGYRKTLLSPEEILLSIEIPYSREGEYFSAFKQASRREDDIAKVTSGMRVLFKPGTTEVQELALCYGGMANRTISALKTTQRQLSKLWKEELLQDVCAGLAEELHLPPDAPGGMVDFRCTLTLSFFFKFYLTVLQKLGQENLEDKCGKLDPTFASATLLFQKDPPADVQLFQEVPKGQSEEDMVGRPLPHLAADMQASGEAVYCDDIPRYENELSLRLVTSTRAHAKIKSIDTSEAKKVPGFVCFISADDVPGSNITGICNDETVFAKDKVTCVGHIIGAVVADTPEHTQRAAQGVKITYEELPAIITIEDAIKNNSFYGPELKIEKGDLKKGFSEADNVVSGEIYIGGQEHFYLETHCTIAVPKGEAGEMELFVSTQNTMKTQSFVAKMLGVPANRIVVRVKRMGGGFGGKETRSTVVSTAVALAAYKTGRPVRCMLDRDEDMLITGGRHPFLARYKVGFMKTGTVVALEVDHFSNVGNTQDLSQSIMERALFHMDNCYKIPNIRGTGRLCKTNLPSNTAFRGFGGPQGMLIAECWMSEVAVTCGMPAEEVRRKNLYKEGDLTHFNQKLEGFTLPRCWEECLASSQYHARKSEVDKFNKENCWKKRGLCIIPTKFGISFTVPFLNQAGALLHVYTDGSVLLTHGGTEMGQGLHTKMVQVASRALKIPTSKIYISETSTNTVPNTSPTAASVSADLNGQAVYAACQTILKRLEPYKKKNPSGSWEDWVTAAYMDTVSLSATGFYRTPNLGYSFETNSGNPFHYFSYGVACSEVEIDCLTGDHKNLRTDIVMDVGSSLNPAIDIGQVEGAFVQGLGLFTLEELHYSPEGSLHTRGPSTYKIPAFGSIPIEFRVSLLRDCPNKKAIYASKAVGEPPLFLAASIFFAIKDAIRAARAQHTGNNVKELFRLDSPATPEKIRNACVDKFTTLCVTGVPENCKPWSVRV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Dog
100
Rabbit
100
Pig
88
Sheep
88
Chicken
88
Zebrafish
75
Horse
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P47989 基板として

Site PTM Type Enzyme
T39 Phosphorylation
T222 Phosphorylation Q00535 (CDK5)
T311 Phosphorylation
T324 Phosphorylation
K443 Methylation
K552 Acetylation
S810 Phosphorylation
Y818 Phosphorylation
T856 Phosphorylation
S1052 Phosphorylation
S1111 Phosphorylation
S1113 Phosphorylation
T1320 Phosphorylation

研究背景

機能:

Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).

PTMs:

Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).

Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).

細胞の位置付け:

Cytoplasm. Peroxisome. Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in milk (at protein level).

サブユニット構造:

Homodimer. Interacts with BTN1A1 (By similarity).

タンパク質ファミリー:

Belongs to the xanthine dehydrogenase family.

研究領域

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Biosynthesis of other secondary metabolites > Caffeine metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

参考文献

1). Sesamol supplementation alleviates nonalcoholic steatohepatitis and atherosclerosis in high-fat, high carbohydrate and high-cholesterol diet-fed rats. Food & Function, 2021 (PubMed: 34606548) [IF=6.1]

Application: WB    Species: Rat    Sample: liver tissue

Fig. 6 Sesamol decreased uric acid levels through the inhibition of XO activity and/or its expression in HF-HCC diet-fed rats. (A–D) The analyses of serum and hepatic XO activities and uric acid levels (samples from 6 rats in each group were analyzed). (E) Representative images of the western blot analysis from hepatic XO expression, and (F) representative images of the IHC analyses from hepatic XO expression (samples from 3 rats in each group were analyzed). Values are expressed as means ± SEM (n = 6); #P < 0.05, # #P < 0.01 and ###P < 0.001, versus control; *P < 0.05 and **P < 0.01, versus HF-HCC.

Application: IHC    Species: Rat    Sample: liver tissue

Fig. 6 Sesamol decreased uric acid levels through the inhibition of XO activity and/or its expression in HF-HCC diet-fed rats. (A–D) The analyses of serum and hepatic XO activities and uric acid levels (samples from 6 rats in each group were analyzed). (E) Representative images of the western blot analysis from hepatic XO expression, and (F) representative images of the IHC analyses from hepatic XO expression (samples from 3 rats in each group were analyzed). Values are expressed as means ± SEM (n = 6); #P < 0.05, # #P < 0.01 and ###P < 0.001, versus control; *P < 0.05 and **P < 0.01, versus HF-HCC.

Application: WB    Species: Rat    Sample:

Fig. 6 Sesamol decreased uric acid levels through the inhibition of XO activity and/or its expression in HF-HCC diet-fed rats. (A–D) The analyses of serum and hepatic XO activities and uric acid levels (samples from 6 rats in each group were analyzed). (E) Representative images of the western blot analysis from hepatic XO expression, and (F) representative images of the IHC analyses from hepatic XO expression (samples from 3 rats in each group were analyzed). Values are expressed as means ± SEM (n = 6); # P < 0.05, # # P < 0.01 and # # # P < 0.001, versus control; *P < 0.05 and **P < 0.01, versus HF-HCC.
2). C1QBP regulates apoptosis of renal cell carcinoma via modulating xanthine dehydrogenase (XDH) mediated ROS generation. International Journal of Medical Sciences, 2022 (PubMed: 35693733) [IF=3.6]

Application: WB    Species: Human    Sample: ACHN and 786-O cells

Figure 2 C1QBP regulates the expression of XDH in RCC. (A) Western blot detected the expression of XDH in ACHN and 786-O cells with stable C1QBP knockdown (left panel) and C1QBP overexpression (right panel). (B) The XDH mRNA was examined by qRT-PCR in ACHN (upper panel) and 786-O (lower panel) cells with stable C1QBP knockdown (left panel) and C1QBP overexpression (right panel). The expression of C1QBP and XDH was detected by western blot both in tumors (T) and adjacent normal tissues (N) of 30 primary RCC patients. (C) Representative 8 pairs of western blot images were shown. (D) The expression of C1QBP (left panel) and XDH (right panel) was normalized with β-actin and quantified by Image J software in 30 primary RCC patients. The analysis was used paired t-test, P = 0.015, P < 0.0001, respectively, n = 30. (E) The expression of C1QBP and XDH was examined by immunohistochemistry staining in a study cohort with 57 pairs of RCC (Tumor) and adjacent normal kidney tissues (Para-tumor). Representative pictures were shown, and brown signals indicated positive staining. Scale bar = 50μm. Statistically significant differences were indicated: *, P < 0.05, **, P < 0.01 and ***, P < 0.001.

Application: IHC    Species: Human    Sample: kidney tissues

Figure 2 C1QBP regulates the expression of XDH in RCC. (A) Western blot detected the expression of XDH in ACHN and 786-O cells with stable C1QBP knockdown (left panel) and C1QBP overexpression (right panel). (B) The XDH mRNA was examined by qRT-PCR in ACHN (upper panel) and 786-O (lower panel) cells with stable C1QBP knockdown (left panel) and C1QBP overexpression (right panel). The expression of C1QBP and XDH was detected by western blot both in tumors (T) and adjacent normal tissues (N) of 30 primary RCC patients. (C) Representative 8 pairs of western blot images were shown. (D) The expression of C1QBP (left panel) and XDH (right panel) was normalized with β-actin and quantified by Image J software in 30 primary RCC patients. The analysis was used paired t-test, P = 0.015, P < 0.0001, respectively, n = 30. (E) The expression of C1QBP and XDH was examined by immunohistochemistry staining in a study cohort with 57 pairs of RCC (Tumor) and adjacent normal kidney tissues (Para-tumor). Representative pictures were shown, and brown signals indicated positive staining. Scale bar = 50μm. Statistically significant differences were indicated: *, P < 0.05, **, P < 0.01 and ***, P < 0.001.

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