PLK4 Antibody - #DF8120
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| 製品: | PLK4 Antibody |
| カタログ: | DF8120 |
| タンパク質の説明: | Rabbit polyclonal antibody to PLK4 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat, Monkey |
| 予測: | Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 95 kDa; 109kD(Calculated). |
| ユニプロット: | O00444 |
| RRID: | AB_2841460 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8120, RRID:AB_2841460.
折りたたみ／展開
PLK 4; PLK-4; PLK4; PLK4_HUMAN; Polo like Kinase 4; Polo-like kinase 4; Protein serine/threonine kinase; SAK; Serine/Threonine Kinase 18; Serine/threonine kinase; Serine/threonine protein kinase 18; Serine/threonine protein kinase PLK4; Serine/threonine protein kinase Sak; Serine/threonine-protein kinase 18; Serine/threonine-protein kinase PLK4; Serine/threonine-protein kinase SAK; Snk Akin Kinase; STK 18; STK18;
免疫原
- O00444 PLK4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATCIGEKIEDFKVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHPSILELYNYFEDSNYVYLVLEMCHNGEMNRYLKNRVKPFSENEARHFMHQIITGMLYLHSHGILHRDLTLSNLLLTRNMNIKIADFGLATQLKMPHEKHYTLCGTPNYISPEIATRSAHGLESDVWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPSFLSIEAKDLIHQLLRRNPADRLSLSSVLDHPFMSRNSSTKSKDLGTVEDSIDSGHATISTAITASSSTSISGSLFDKRRLLIGQPLPNKMTVFPKNKSSTDFSSSGDGNSFYTQWGNQETSNSGRGRVIQDAEERPHSRYLRRAYSSDRSGTSNSQSQAKTYTMERCHSAEMLSVSKRSGGGENEERYSPTDNNANIFNFFKEKTSSSSGSFERPDNNQALSNHLCPGKTPFPFADPTPQTETVQQWFGNLQINAHLRKTTEYDSISPNRDFQGHPDLQKDTSKNAWTDTKVKKNSDASDNAHSVKQQNTMKYMTALHSKPEIIQQECVFGSDPLSEQSKTRGMEPPWGYQNRTLRSITSPLVAHRLKPIRQKTKKAVVSILDSEEVCVELVKEYASQEYVKEVLQISSDGNTITIYYPNGGRGFPLADRPPSPTDNISRYSFDNLPEKYWRKYQYASRFVQLVRSKSPKITYFTRYAKCILMENSPGADFEVWFYDGVKIHKTEDFIQVIEKTGKSYTLKSESEVNSLKEEIKMYMDHANEGHRICLALESIISEEERKTRSAPFFPIIIGRKPGSTSSPKALSPPPSVDSNYPTRERASFNRMVMHSAASPTQAPILNPSMVTNEGLGLTTTASGTDISSNSLKDCLPKSAQLLKSVFVKNVGWATQLTSGAVWVQFNDGSQLVVQAGVSSISYTSPNGQTTRYGENEKLPDYIKQKLQCLSSILLMFSNPTPNFH
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O00444 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K8 | Ubiquitination | Uniprot | |
| K13 | Ubiquitination | Uniprot | |
| S22 | Phosphorylation | O00444 (PLK4) | Uniprot |
| K45 | Acetylation | Uniprot | |
| K46 | Acetylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| T170 | Phosphorylation | O00444 (PLK4) | Uniprot |
| T174 | Phosphorylation | Uniprot | |
| Y177 | Phosphorylation | O00444 (PLK4) | Uniprot |
| S255 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | O00444 (PLK4) | Uniprot |
| S285 | Phosphorylation | O00444 (PLK4) | Uniprot |
| T289 | Phosphorylation | O00444 (PLK4) | Uniprot |
| S291 | Phosphorylation | O00444 (PLK4) | Uniprot |
| T292 | Phosphorylation | O00444 (PLK4) | Uniprot |
| T295 | Phosphorylation | O00444 (PLK4) | Uniprot |
| S297 | Phosphorylation | O00444 (PLK4) | Uniprot |
| S298 | Phosphorylation | O00444 (PLK4) | Uniprot |
| T300 | Phosphorylation | O00444 (PLK4) | Uniprot |
| S301 | Phosphorylation | O00444 (PLK4) | Uniprot |
| S303 | Phosphorylation | O00444 (PLK4) | Uniprot |
| S305 | Phosphorylation | O00444 (PLK4) | Uniprot |
| K321 | Ubiquitination | Uniprot | |
| T323 | Phosphorylation | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| K329 | Ubiquitination | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| T345 | Phosphorylation | Uniprot | |
| R357 | Methylation | Uniprot | |
| Y377 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| K392 | Ubiquitination | Uniprot | |
| Y394 | Phosphorylation | Uniprot | |
| S401 | Phosphorylation | Uniprot | |
| S406 | Phosphorylation | Uniprot | |
| Y420 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| Y495 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| K512 | Ubiquitination | Uniprot | |
| K538 | Acetylation | Uniprot | |
| T547 | Phosphorylation | Uniprot | |
| S551 | Phosphorylation | Uniprot | |
| Y582 | Phosphorylation | Uniprot | |
| S589 | Phosphorylation | Uniprot | |
| T591 | Phosphorylation | Uniprot | |
| S592 | Phosphorylation | Uniprot | |
| S665 | Phosphorylation | Uniprot | |
| S671 | Phosphorylation | Uniprot | |
| S749 | Phosphorylation | Uniprot | |
| T751 | Phosphorylation | Uniprot | |
| K753 | Ubiquitination | Uniprot | |
| S817 | Phosphorylation | Uniprot | |
| S821 | Phosphorylation | Uniprot |
PTMs - O00444 酵素として
| Substrate | Site | Source |
|---|---|---|
| O00444 (PLK4) | S22 | Uniprot |
| O00444 (PLK4) | T170 | Uniprot |
| O00444 (PLK4) | Y177 | Uniprot |
| O00444 (PLK4) | S282 | Uniprot |
| O00444 (PLK4) | S285 | Uniprot |
| O00444 (PLK4) | T289 | Uniprot |
| O00444 (PLK4) | S291 | Uniprot |
| O00444 (PLK4) | T292 | Uniprot |
| O00444 (PLK4) | T295 | Uniprot |
| O00444 (PLK4) | S297 | Uniprot |
| O00444 (PLK4) | S298 | Uniprot |
| O00444 (PLK4) | T300 | Uniprot |
| O00444 (PLK4) | S301 | Uniprot |
| O00444 (PLK4) | S303 | Uniprot |
| O00444 (PLK4) | S305 | Uniprot |
| O96004 (HAND1) | T107 | Uniprot |
| O96004 (HAND1) | S109 | Uniprot |
| Q15154 (PCM1) | S372 | Uniprot |
| Q969U6 (FBXW5) | S151 | Uniprot |
| Q96RT7 (TUBGCP6) | S392 | Uniprot |
| Q96RT7 (TUBGCP6) | S1041 | Uniprot |
| Q96RT7 (TUBGCP6) | S1060 | Uniprot |
| Q96RT7 (TUBGCP6) | S1068 | Uniprot |
| Q96RT7 (TUBGCP6) | S1087 | Uniprot |
| Q96RT7 (TUBGCP6) | S1095 | Uniprot |
| Q96RT7 (TUBGCP6) | S1114 | Uniprot |
| Q96RT7 (TUBGCP6) | S1122 | Uniprot |
| Q96RT7 (TUBGCP6) | S1141 | Uniprot |
| Q96RT7 (TUBGCP6) | S1149 | Uniprot |
| Q96RT7 (TUBGCP6) | S1168 | Uniprot |
| Q96RT7 (TUBGCP6) | S1176 | Uniprot |
| Q96RT7 (TUBGCP6) | S1195 | Uniprot |
| Q96RT7 (TUBGCP6) | S1203 | Uniprot |
| Q96RT7 (TUBGCP6) | S1222 | Uniprot |
| Q96RT7 (TUBGCP6) | S1230 | Uniprot |
| Q96RT7 (TUBGCP6) | S1249 | Uniprot |
| Q96RT7 (TUBGCP6) | S1257 | Uniprot |
| Q96RT7 (TUBGCP6) | S1437 | Uniprot |
| Q96RT7 (TUBGCP6) | S1465 | Uniprot |
| Q9HC77 (CENPJ) | S595 | Uniprot |
研究背景
Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2. Required for the recruitment of STIL to the centriole and for STIL-mediated centriole amplification.
Acetylation by KAT2A and KAT2B impairs kinase activity by shifting the kinase to an inactive conformation.
Ubiquitinated; leading to its degradation by the proteasome.
Tyrosine-phosphorylated by TEC.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Nucleus>Nucleolus. Cleavage furrow.
Note: Component of the deuterosome, a structure that promotes de novo centriole amplification in multiciliated cells that can generate more than 100 centrioles. Associates with centrioles throughout the cell cycle. According to PubMed:16244668, it is not present at cleavage furrows.
Homodimer (By similarity). Interacts with CEP152 (via N-terminus). Interacts with CEP78; this interaction may be important for proper PLK4 localization to the centriole and PLK4-induced overduplication of centrioles.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
研究領域
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
参考文献
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