LCAT Antibody - #DF8167
| 製品: | LCAT Antibody |
| カタログ: | DF8167 |
| タンパク質の説明: | Rabbit polyclonal antibody to LCAT |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 50 kDa; 50kD(Calculated). |
| ユニプロット: | P04180 |
| RRID: | AB_2841488 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8167, RRID:AB_2841488.
折りたたみ／展開
LCAT; LCAT_HUMAN; Lecithin cholesterol acyltransferase; Lecithin-cholesterol acyltransferase; Phosphatidylcholine sterol acyltransferase; Phosphatidylcholine-sterol acyltransferase; Phospholipid cholesterol acyltransferase; Phospholipid-cholesterol acyltransferase;
免疫原
Detected in blood plasma (PubMed:3458198, PubMed:8820107, PubMed:10222237). Detected in cerebral spinal fluid (at protein level) (PubMed:10222237). Detected in liver (PubMed:3797244, PubMed:3458198). Expressed mainly in brain, liver and testes.
- P04180 LCAT_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGPPGSPWQWVTLLLGLLLPPAAPFWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTIWLDLNMFLPLGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGHSLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLKEEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDLLAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQGRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P04180 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N44 | N-Glycosylation | Uniprot | |
| N108 | N-Glycosylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| Y213 | Phosphorylation | Uniprot | |
| N296 | N-Glycosylation | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| T377 | Phosphorylation | Uniprot | |
| N408 | N-Glycosylation | Uniprot | |
| T431 | O-Glycosylation | Uniprot | |
| S433 | O-Glycosylation | Uniprot |
研究背景
Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms.
O- and N-glycosylated. O-glycosylation on Thr-431 and Ser-433 consists of sialylated galactose beta 1-->3N-acetylgalactosamine structures. N-glycosylated sites contain sialylated triantennary and/or biantennary complex structures.
Secreted.
Note: Secreted into blood plasma (PubMed:3458198, PubMed:8820107, PubMed:10222237). Produced in astrocytes and secreted into cerebral spinal fluid (CSF).
Detected in blood plasma. Detected in cerebral spinal fluid (at protein level). Detected in liver. Expressed mainly in brain, liver and testes.
Belongs to the AB hydrolase superfamily. Lipase family.
研究領域
· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.
· Organismal Systems > Digestive system > Cholesterol metabolism.
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