ABCA1 Antibody - #DF8233

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製品: ABCA1 Antibody
カタログ: DF8233
タンパク質の説明: Rabbit polyclonal antibody to ABCA1
アプリケーション: WB
反応性: Human, Mouse
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 254 kDa; 254kD(Calculated).
ユニプロット: O95477
RRID: AB_2841530

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 100ul $280 在庫あり
 200ul $350 在庫あり

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
ABCA1 Antibody detects endogenous levels of total ABCA1.
RRID:
AB_2841530
引用形式: Affinity Biosciences Cat# DF8233, RRID:AB_2841530.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ABC 1; ABC Transporter 1; ABC-1; ABC1; ABCA 1; ABCA1; ABCA1_HUMAN; ATP binding Cassette 1; ATP binding cassette sub family A ABC1 member 1; ATP binding cassette sub family A member 1; ATP binding Cassette Transporter 1; ATP-binding cassette 1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1; CERP; Cholesterol efflux regulatory protein; FLJ14958; HDLDT1; Membrane bound; MGC164864; MGC165011; TD; TGD;

免疫原

免疫原:
Uniprot:

O95477(ABCA1_HUMAN) >>Visit HPA database.

遺伝子(ID):
ABCA1(19)
発現特異性:
O95477 ABCA1_HUMAN:

Widely expressed, but most abundant in macrophages.

タンパク質配列:
MACWPQLRLLLWKNLTFRRRQTCQLLLEVAWPLFIFLILISVRLSYPPYEQHECHFPNKAMPSAGTLPWVQGIICNANNPCFRYPTPGEAPGVVGNFNKSIVARLFSDARRLLLYSQKDTSMKDMRKVLRTLQQIKKSSSNLKLQDFLVDNETFSGFLYHNLSLPKSTVDKMLRADVILHKVFLQGYQLHLTSLCNGSKSEEMIQLGDQEVSELCGLPREKLAAAERVLRSNMDILKPILRTLNSTSPFPSKELAEATKTLLHSLGTLAQELFSMRSWSDMRQEVMFLTNVNSSSSSTQIYQAVSRIVCGHPEGGGLKIKSLNWYEDNNYKALFGGNGTEEDAETFYDNSTTPYCNDLMKNLESSPLSRIIWKALKPLLVGKILYTPDTPATRQVMAEVNKTFQELAVFHDLEGMWEELSPKIWTFMENSQEMDLVRMLLDSRDNDHFWEQQLDGLDWTAQDIVAFLAKHPEDVQSSNGSVYTWREAFNETNQAIRTISRFMECVNLNKLEPIATEVWLINKSMELLDERKFWAGIVFTGITPGSIELPHHVKYKIRMDIDNVERTNKIKDGYWDPGPRADPFEDMRYVWGGFAYLQDVVEQAIIRVLTGTEKKTGVYMQQMPYPCYVDDIFLRVMSRSMPLFMTLAWIYSVAVIIKGIVYEKEARLKETMRIMGLDNSILWFSWFISSLIPLLVSAGLLVVILKLGNLLPYSDPSVVFVFLSVFAVVTILQCFLISTLFSRANLAAACGGIIYFTLYLPYVLCVAWQDYVGFTLKIFASLLSPVAFGFGCEYFALFEEQGIGVQWDNLFESPVEEDGFNLTTSVSMMLFDTFLYGVMTWYIEAVFPGQYGIPRPWYFPCTKSYWFGEESDEKSHPGSNQKRISEICMEEEPTHLKLGVSIQNLVKVYRDGMKVAVDGLALNFYEGQITSFLGHNGAGKTTTMSILTGLFPPTSGTAYILGKDIRSEMSTIRQNLGVCPQHNVLFDMLTVEEHIWFYARLKGLSEKHVKAEMEQMALDVGLPSSKLKSKTSQLSGGMQRKLSVALAFVGGSKVVILDEPTAGVDPYSRRGIWELLLKYRQGRTIILSTHHMDEADVLGDRIAIISHGKLCCVGSSLFLKNQLGTGYYLTLVKKDVESSLSSCRNSSSTVSYLKKEDSVSQSSSDAGLGSDHESDTLTIDVSAISNLIRKHVSEARLVEDIGHELTYVLPYEAAKEGAFVELFHEIDDRLSDLGISSYGISETTLEEIFLKVAEESGVDAETSDGTLPARRNRRAFGDKQSCLRPFTEDDAADPNDSDIDPESRETDLLSGMDGKGSYQVKGWKLTQQQFVALLWKRLLIARRSRKGFFAQIVLPAVFVCIALVFSLIVPPFGKYPSLELQPWMYNEQYTFVSNDAPEDTGTLELLNALTKDPGFGTRCMEGNPIPDTPCQAGEEEWTTAPVPQTIMDLFQNGNWTMQNPSPACQCSSDKIKKMLPVCPPGAGGLPPPQRKQNTADILQDLTGRNISDYLVKTYVQIIAKSLKNKIWVNEFRYGGFSLGVSNTQALPPSQEVNDAIKQMKKHLKLAKDSSADRFLNSLGRFMTGLDTKNNVKVWFNNKGWHAISSFLNVINNAILRANLQKGENPSHYGITAFNHPLNLTKQQLSEVALMTTSVDVLVSICVIFAMSFVPASFVVFLIQERVSKAKHLQFISGVKPVIYWLSNFVWDMCNYVVPATLVIIIFICFQQKSYVSSTNLPVLALLLLLYGWSITPLMYPASFVFKIPSTAYVVLTSVNLFIGINGSVATFVLELFTDNKLNNINDILKSVFLIFPHFCLGRGLIDMVKNQAMADALERFGENRFVSPLSWDLVGRNLFAMAVEGVVFFLITVLIQYRFFIRPRPVNAKLSPLNDEDEDVRRERQRILDGGGQNDILEIKELTKIYRRKRKPAVDRICVGIPPGECFGLLGVNGAGKSSTFKMLTGDTTVTRGDAFLNKNSILSNIHEVHQNMGYCPQFDAITELLTGREHVEFFALLRGVPEKEVGKVGEWAIRKLGLVKYGEKYAGNYSGGNKRKLSTAMALIGGPPVVFLDEPTTGMDPKARRFLWNCALSVVKEGRSVVLTSHSMEECEALCTRMAIMVNGRFRCLGSVQHLKNRFGDGYTIVVRIAGSNPDLKPVQDFFGLAFPGSVLKEKHRNMLQYQLPSSLSSLARIFSILSQSKKRLHIEDYSVSQTTLDQVFVNFAKDQSDDDHLKDLSLHKNQTVVDVAVLTSFLQDEKVKESYV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
92
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95477 基板として

Site PTM Type Enzyme
N98 N-Glycosylation
S116 Phosphorylation
S231 Phosphorylation
N244 N-Glycosylation
Y325 Phosphorylation
S884 Phosphorylation
S1031 Phosphorylation
S1042 Phosphorylation P17612 (PRKACA)
S1145 Phosphorylation
Y1206 Phosphorylation
T1242 Phosphorylation P68400 (CSNK2A1)
T1243 Phosphorylation P68400 (CSNK2A1)
S1255 Phosphorylation P68400 (CSNK2A1)
S1262 Phosphorylation
T1286 Phosphorylation
S1296 Phosphorylation
T1305 Phosphorylation
S1536 Phosphorylation
S1540 Phosphorylation
S1548 Phosphorylation
T1582 Phosphorylation
T1586 Phosphorylation
K2050 Acetylation
S2054 Phosphorylation P00533 (EGFR) , P17612 (PRKACA)
S2101 Phosphorylation
S2234 Phosphorylation

研究背景

機能:

Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP. Thereby, participates to phospholipids transfer to apoliproteins to form nascent high density lipoproteins/HDLs. Transports preferentially phosphatidylcholine over phosphatidylserine. May play a similar role in the efflux of intracellular cholesterol to apoliproteins and the formation of nascent high density lipoproteins/HDLs.

PTMs:

Phosphorylation on Ser-2054 regulates phospholipid efflux.

Palmitoylation by DHHC8 is essential for membrane localization.

細胞の位置付け:

Membrane>Multi-pass membrane protein. Cell membrane. Endosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed, but most abundant in macrophages.

サブユニット構造:

Interacts with MEGF10. May interact with APOE1; functionally associated with APOE1 in the biogenesis of HDLs.

タンパク質ファミリー:

Multifunctional polypeptide with two homologous halves, each containing a hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.

Belongs to the ABC transporter superfamily. ABCA family.

研究領域

· Environmental Information Processing > Membrane transport > ABC transporters.

· Organismal Systems > Digestive system > Fat digestion and absorption.

· Organismal Systems > Digestive system > Cholesterol metabolism.

参考文献

1). Yin-xing-tong-mai decoction attenuates atherosclerosis via activating PPARγ-LXRα-ABCA1/ABCG1 pathway. Pharmacological research, 2021 (PubMed: 33932607) [IF=9.3]
2). MaiJiTong granule attenuates atherosclerosis by reducing ferroptosis via activating STAT6-mediated inhibition of DMT1 and SOCS1/p53 pathways in LDLR-/- mice. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38569295) [IF=7.9]
3). Proteus mirabilis Vesicles Induce Mitochondrial Apoptosis by Regulating miR96-5p/Abca1 to Inhibit Osteoclastogenesis and Bone Loss. Frontiers in Immunology, 2022 (PubMed: 35242136) [IF=7.3]

Application: WB    Species: mouse    Sample:

FIGURE 6| Effect of P.M OMVs on miR96/Abca1 mediated mitochondria-dependent apoptosis.(J, K) Expression of Abca1 after 0.15 μg/ml of P.M OMV treatment by RT-qPCR and WB.
4). Fatty acid binding protein 3 deficiency limits atherosclerosis development via macrophage foam cell formation inhibition. EXPERIMENTAL CELL RESEARCH, 2021 (PubMed: 34370993) [IF=3.7]

Application: WB    Species: Mice    Sample: macrophages

Fig. 3. FABP3 knockdown reduced macrophage foam cell formation. Murine peritoneal macrophages were maintained in the special culture medium at 37 ◦ C in a 5 % CO 2 atmosphere. The cells were infected with lentivirus carrying shFABP3 or shRNA NC for 24 h and subsequently exposed with ox-LDL (50 μ g/ml) for 24 h. (A) Representative Oil Red O-stained images of macrophages and quantification of the staining. (B) Immunoblots of SRA1, CD36, SRB1, ABCA1, and ABCG1 in macrophages. (C) Quantification of relative mRNA expression of SRA1, CD36, SRB1, ABCA1, and ABCG1. **p < 0.01 denote significant differences between the Control and the ox-LDL groups, && p < 0.01 denote significant differences between the ox-LDL shRNA NC and ox-LDL + shFABP3 groups.

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