EEA1 Antibody - #DF8248
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8248, RRID:AB_2841542.
折りたたみ／展開
Early endosome antigen 1; Early endosome antigen 1, 162kD; Early endosome associated protein; EEA 1; EEA1; EEA1_HUMAN; Endosome associated protein p162; Endosome-associated protein p162; MST105; MSTP105; ZFYVE2; Zinc finger FYVE domain containing protein 2; Zinc finger FYVE domain-containing protein 2;
免疫原
- Q15075 EEA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQCMKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKWYSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQEVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLHQKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQQQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADLQLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQVTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKEKVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVTAKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDLQQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKKILKQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKNSKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLKLELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQKTELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQDLKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQDIQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQLLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEKEVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDACFNDLQG
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15075 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T9 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| T25 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| K158 | Acetylation | Uniprot | |
| K164 | Acetylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| T235 | Phosphorylation | Uniprot | |
| T241 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| Y286 | Phosphorylation | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| T301 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| S382 | Phosphorylation | Uniprot | |
| K422 | Ubiquitination | Uniprot | |
| K544 | Ubiquitination | Uniprot | |
| K552 | Ubiquitination | Uniprot | |
| T560 | Phosphorylation | Uniprot | |
| K592 | Ubiquitination | Uniprot | |
| S618 | Phosphorylation | Uniprot | |
| T621 | Phosphorylation | Uniprot | |
| S628 | Phosphorylation | Uniprot | |
| K634 | Acetylation | Uniprot | |
| S638 | Phosphorylation | Uniprot | |
| K645 | Acetylation | Uniprot | |
| T761 | Phosphorylation | Uniprot | |
| K824 | Ubiquitination | Uniprot | |
| T836 | Phosphorylation | Uniprot | |
| T837 | Phosphorylation | Uniprot | |
| K848 | Methylation | Uniprot | |
| T853 | Phosphorylation | Uniprot | |
| S856 | Phosphorylation | Uniprot | |
| T857 | Phosphorylation | Uniprot | |
| S863 | Phosphorylation | Uniprot | |
| K884 | Ubiquitination | Uniprot | |
| K895 | Ubiquitination | Uniprot | |
| T908 | Phosphorylation | Uniprot | |
| S926 | Phosphorylation | Uniprot | |
| K995 | Ubiquitination | Uniprot | |
| S1015 | Phosphorylation | Uniprot | |
| Y1021 | Phosphorylation | Uniprot | |
| S1024 | Phosphorylation | Uniprot | |
| T1027 | Phosphorylation | Uniprot | |
| K1029 | Ubiquitination | Uniprot | |
| K1125 | Ubiquitination | Uniprot | |
| K1133 | Acetylation | Uniprot | |
| K1138 | Acetylation | Uniprot | |
| K1142 | Acetylation | Uniprot | |
| T1159 | Phosphorylation | Uniprot | |
| K1178 | Ubiquitination | Uniprot | |
| K1183 | Ubiquitination | Uniprot | |
| T1252 | Phosphorylation | Uniprot | |
| S1265 | Phosphorylation | Uniprot | |
| K1312 | Ubiquitination | Uniprot | |
| K1319 | Ubiquitination | Uniprot | |
| K1340 | Ubiquitination | Uniprot | |
| T1392 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
研究背景
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Cytoplasm. Early endosome membrane>Peripheral membrane protein.
Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding. Interacts with RAB31. Interacts with ERBB2. Interacts with SAMD9 AND SAMD9L. May interact with PLEKHF2.
The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
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