OS9 Antibody - #DF8249
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8249, RRID:AB_2841543.
折りたたみ／展開
Amplified in osteosarcoma 9; amplified in osteosarcoma; ERLEC2; OS-9; Os9; OS9_HUMAN; Osteosarcoma amplified 9 endoplasmic reticulum lectin; Protein OS-9;
免疫原
Ubiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma SJSA-1 and rhabdomyosarcoma Rh30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.
- Q13438 OS9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAETLLSSLLGLLLLGLLLPASLTGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTEEDPEHRVRVRVTKLRLGGPNQDLTVLEMKRENPQLKQIEGLVKELLEREGLTAAGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPGAEEAQKERQRQKELESNYRRVWGSPGGEGTGDLDEFDF
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13438 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K113 | Ubiquitination | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K180 | Ubiquitination | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| K295 | Ubiquitination | Uniprot | |
| S300 | Phosphorylation | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| K373 | Ubiquitination | Uniprot | |
| K380 | Ubiquitination | Uniprot | |
| K402 | Ubiquitination | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| K457 | Ubiquitination | Uniprot | |
| K473 | Ubiquitination | Uniprot | |
| K481 | Ubiquitination | Uniprot | |
| K499 | Ubiquitination | Uniprot | |
| K507 | Ubiquitination | Uniprot | |
| S509 | Phosphorylation | Uniprot | |
| K514 | Ubiquitination | Uniprot | |
| S529 | O-Glycosylation | Uniprot | |
| S529 | Phosphorylation | Uniprot | |
| T533 | O-Glycosylation | Uniprot | |
| K563 | Ubiquitination | Uniprot | |
| K570 | Ubiquitination | Uniprot | |
| K577 | Ubiquitination | Uniprot | |
| K594 | Ubiquitination | Uniprot | |
| T603 | Phosphorylation | Uniprot | |
| T611 | Phosphorylation | Uniprot | |
| K619 | Ubiquitination | Uniprot | |
| K635 | Ubiquitination | Uniprot | |
| K641 | Ubiquitination | Uniprot |
研究背景
Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.
Intramolecular disulfide bonds.
Isoform 1 and isoform 2 are N-glycosylated.
Endoplasmic reticulum lumen.
Ubiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma SJSA-1 and rhabdomyosarcoma Rh30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.
Interacts (via C-terminus) with CPNE6 (via second C2 domain); this interaction occurs in a calcium-dependent manner in vitro (By similarity). Probably part of the HRD1 ubiquitin ligase complex composed at least of SYVN1/HRD1 and SEL1L with which it interacts directly. Through this complex it may interact with ERLEC1 and HSPA5. Interacts with DERL2. Interacts with HSP90B1. Interacts with CREB3. Interacts with SYVN1.
Belongs to the OS-9 family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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