UIMC1 Antibody - #DF8264
| 製品: | UIMC1 Antibody |
| カタログ: | DF8264 |
| タンパク質の説明: | Rabbit polyclonal antibody to UIMC1 |
| アプリケーション: | WB IHC |
| 反応性: | Human |
| 予測: | Horse, Rabbit, Dog |
| 分子量: | 80 kDa; 80kD(Calculated). |
| ユニプロット: | Q96RL1 |
| RRID: | AB_2841553 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8264, RRID:AB_2841553.
折りたたみ／展開
BRCA1-A complex subunit RAP80; Nuclear zinc finger protein RAP80; OTTHUMP00000161441; OTTHUMP00000223372; OTTHUMP00000223374; RAP80; Receptor associated protein 80; Receptor-associated protein 80; Retinoid X receptor interacting protein 110; Retinoid x receptor interacting protein; Retinoid X receptor-interacting protein 110; RIP110; Rxrip110; Ubiquitin interaction motif containing 1; Ubiquitin interaction motif containing protein 1; Ubiquitin interaction motif-containing protein 1; UIMC1; UIMC1_HUMAN; X2HRIP110;
免疫原
Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.
- Q96RL1 UIMC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPRRKKKVKEVSESRNLEKKDVETTSSVSVKRKRRLEDAFIVISDSDGEEPKEENGLQKTKTKQSNRAKCLAKRKIAQMTEEEQFALALKMSEQEAREVNSQEEEEEELLRKAIAESLNSCRPSDASATRSRPLATGPSSQSHQEKTTDSGLTEGIWQLVPPSLFKGSHISQGNEAEEREEPWDHTEKTEEEPVSGSSGSWDQSSQPVFENVNVKSFDRCTGHSAEHTQCGKPQESTGRGSAFLKAVQGSGDTSRHCLPTLADAKGLQDTGGTVNYFWGIPFCPDGVDPNQYTKVILCQLEVYQKSLKMAQRQLLNKKGFGEPVLPRPPSLIQNECGQGEQASEKNECISEDMGDEDKEERQESRASDWHSKTKDFQESSIKSLKEKLLLEEEPTTSHGQSSQGIVEETSEEGNSVPASQSVAALTSKRSLVLMPESSAEEITVCPETQLSSSETFDLEREVSPGSRDILDGVRIIMADKEVGNKEDAEKEVAISTFSSSNQVSCPLCDQCFPPTKIERHAMYCNGLMEEDTVLTRRQKEAKTKSDSGTAAQTSLDIDKNEKCYLCKSLVPFREYQCHVDSCLQLAKADQGDGPEGSGRACSTVEGKWQQRLKNPKEKGHSEGRLLSFLEQSEHKTSDADIKSSETGAFRVPSPGMEEAGCSREMQSSFTRRDLNESPVKSFVSISEATDCLVDFKKQVTVQPGSRTRTKAGRGRRRKF
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96RL1 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K9 | Sumoylation | Uniprot | |
| K9 | Ubiquitination | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| K20 | Sumoylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| T24 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| K31 | Sumoylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| S44 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S46 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K59 | Ubiquitination | Uniprot | |
| K75 | Sumoylation | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| K90 | Sumoylation | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| R97 | Methylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| T136 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| T147 | Phosphorylation | Uniprot | |
| K166 | Sumoylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| S198 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Q13315 (ATM) , Q13535 (ATR) | Uniprot |
| K215 | Ubiquitination | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| R239 | Methylation | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| K245 | Methylation | Uniprot | |
| K245 | Sumoylation | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K265 | Ubiquitination | Uniprot | |
| K305 | Ubiquitination | Uniprot | |
| K308 | Sumoylation | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| K358 | Ubiquitination | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| K374 | Ubiquitination | Uniprot | |
| S380 | Phosphorylation | Uniprot | |
| K382 | Sumoylation | Uniprot | |
| K382 | Ubiquitination | Uniprot | |
| S383 | Phosphorylation | Uniprot | |
| K387 | Ubiquitination | Uniprot | |
| T395 | Phosphorylation | Uniprot | |
| T396 | Phosphorylation | Uniprot | |
| S402 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S415 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| K428 | Ubiquitination | Uniprot | |
| S451 | Phosphorylation | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| S466 | Phosphorylation | Uniprot | |
| K480 | Sumoylation | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| K485 | Sumoylation | Uniprot | |
| K485 | Ubiquitination | Uniprot | |
| S498 | Phosphorylation | Uniprot | |
| K544 | Sumoylation | Uniprot | |
| K544 | Ubiquitination | Uniprot | |
| S545 | Phosphorylation | Uniprot | |
| S547 | Phosphorylation | Uniprot | |
| S554 | Phosphorylation | Uniprot | |
| K559 | Sumoylation | Uniprot | |
| K559 | Ubiquitination | Uniprot | |
| K562 | Sumoylation | Uniprot | |
| K562 | Ubiquitination | Uniprot | |
| K567 | Ubiquitination | Uniprot | |
| K587 | Sumoylation | Uniprot | |
| K587 | Ubiquitination | Uniprot | |
| S597 | Phosphorylation | Uniprot | |
| S602 | Phosphorylation | Uniprot | |
| K607 | Sumoylation | Uniprot | |
| K607 | Ubiquitination | Uniprot | |
| K618 | Ubiquitination | Uniprot | |
| S627 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S632 | Phosphorylation | Uniprot | |
| K635 | Sumoylation | Uniprot | |
| K635 | Ubiquitination | Uniprot | |
| K642 | Ubiquitination | Uniprot | |
| S653 | Phosphorylation | Uniprot | |
| S662 | Phosphorylation | Uniprot | |
| S668 | Phosphorylation | Uniprot | |
| S677 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K680 | Acetylation | Uniprot | |
| S681 | Phosphorylation | Uniprot | |
| K696 | Acetylation | Uniprot | |
| K696 | Ubiquitination | Uniprot | |
| K697 | Ubiquitination | Uniprot | |
| T700 | Phosphorylation | Uniprot | |
| S705 | Phosphorylation | Uniprot |
研究背景
Ubiquitin-binding protein. Specifically recognizes and binds 'Lys-63'-linked ubiquitin Ref.37). Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
Sumoylated.
Phosphorylated upon DNA damage by ATM or ATR.
Nucleus.
Note: Localizes at sites of DNA damage at double-strand breaks (DSBs).
Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.
Interacts with TSP57 (By similarity). Component of the ARISC complex, at least composed of UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with ABRAXAS1. Interacts with ESR1, NR6A1 and UBE2I.
The tandem UIM domains form a continuous 60 Angstrom-long alpha-helix and mediate binding to 'Lys-63'-linked ubiquitins. UIM1 and UIM2 bind to the proximal and distal ubiquitin moieties and recognize an 'Ile-44'-centered hydrophobic patch. Since UIMs don't interact with the 'Lys-63' isopeptide bond the UIM-linker region between the 2 UIM domains determines the selectivity for 'Lys-63'-linkage, and its length is very important for specificity.
The Abraxas-interacting region (AIR) mediates the interaction with ABRAXAS1.
Belongs to the RAP80 family.
研究領域
· Genetic Information Processing > Replication and repair > Homologous recombination.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.