製品: UIMC1 Antibody
カタログ: DF8264
タンパク質の説明: Rabbit polyclonal antibody to UIMC1
アプリケーション: WB IHC
反応性: Human
予測: Horse, Rabbit, Dog
分子量: 80 kDa; 80kD(Calculated).
ユニプロット: Q96RL1
RRID: AB_2841553

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Horse(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
UIMC1 Antibody detects endogenous levels of total UIMC1.
RRID:
AB_2841553
引用形式: Affinity Biosciences Cat# DF8264, RRID:AB_2841553.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

BRCA1-A complex subunit RAP80; Nuclear zinc finger protein RAP80; OTTHUMP00000161441; OTTHUMP00000223372; OTTHUMP00000223374; RAP80; Receptor associated protein 80; Receptor-associated protein 80; Retinoid X receptor interacting protein 110; Retinoid x receptor interacting protein; Retinoid X receptor-interacting protein 110; RIP110; Rxrip110; Ubiquitin interaction motif containing 1; Ubiquitin interaction motif containing protein 1; Ubiquitin interaction motif-containing protein 1; UIMC1; UIMC1_HUMAN; X2HRIP110;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q96RL1 UIMC1_HUMAN:

Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.

タンパク質配列:
MPRRKKKVKEVSESRNLEKKDVETTSSVSVKRKRRLEDAFIVISDSDGEEPKEENGLQKTKTKQSNRAKCLAKRKIAQMTEEEQFALALKMSEQEAREVNSQEEEEEELLRKAIAESLNSCRPSDASATRSRPLATGPSSQSHQEKTTDSGLTEGIWQLVPPSLFKGSHISQGNEAEEREEPWDHTEKTEEEPVSGSSGSWDQSSQPVFENVNVKSFDRCTGHSAEHTQCGKPQESTGRGSAFLKAVQGSGDTSRHCLPTLADAKGLQDTGGTVNYFWGIPFCPDGVDPNQYTKVILCQLEVYQKSLKMAQRQLLNKKGFGEPVLPRPPSLIQNECGQGEQASEKNECISEDMGDEDKEERQESRASDWHSKTKDFQESSIKSLKEKLLLEEEPTTSHGQSSQGIVEETSEEGNSVPASQSVAALTSKRSLVLMPESSAEEITVCPETQLSSSETFDLEREVSPGSRDILDGVRIIMADKEVGNKEDAEKEVAISTFSSSNQVSCPLCDQCFPPTKIERHAMYCNGLMEEDTVLTRRQKEAKTKSDSGTAAQTSLDIDKNEKCYLCKSLVPFREYQCHVDSCLQLAKADQGDGPEGSGRACSTVEGKWQQRLKNPKEKGHSEGRLLSFLEQSEHKTSDADIKSSETGAFRVPSPGMEEAGCSREMQSSFTRRDLNESPVKSFVSISEATDCLVDFKKQVTVQPGSRTRTKAGRGRRRKF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
70
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96RL1 基板として

Site PTM Type Enzyme
K9 Sumoylation
K9 Ubiquitination
S12 Phosphorylation
K19 Ubiquitination
K20 Sumoylation
K20 Ubiquitination
T24 Phosphorylation
S26 Phosphorylation
S27 Phosphorylation
S29 Phosphorylation
K31 Sumoylation
K31 Ubiquitination
S44 Phosphorylation P68400 (CSNK2A1)
S46 Phosphorylation P68400 (CSNK2A1)
K59 Ubiquitination
K75 Sumoylation
K75 Ubiquitination
K90 Sumoylation
K90 Ubiquitination
R97 Methylation
S101 Phosphorylation
K112 Ubiquitination
T136 Phosphorylation
S139 Phosphorylation
S140 Phosphorylation
S142 Phosphorylation
K146 Ubiquitination
T147 Phosphorylation
K166 Sumoylation
K166 Ubiquitination
S171 Phosphorylation
K188 Ubiquitination
S195 Phosphorylation
S197 Phosphorylation
S198 Phosphorylation
S200 Phosphorylation
S205 Phosphorylation Q13315 (ATM) , Q13535 (ATR)
K215 Ubiquitination
S224 Phosphorylation
K232 Ubiquitination
R239 Methylation
S241 Phosphorylation
K245 Methylation
K245 Sumoylation
K245 Ubiquitination
K265 Ubiquitination
K305 Ubiquitination
K308 Sumoylation
K308 Ubiquitination
K318 Ubiquitination
S330 Phosphorylation
S350 Phosphorylation
K358 Ubiquitination
K372 Ubiquitination
K374 Ubiquitination
S380 Phosphorylation
K382 Sumoylation
K382 Ubiquitination
S383 Phosphorylation
K387 Ubiquitination
T395 Phosphorylation
T396 Phosphorylation
S402 Phosphorylation Q13315 (ATM)
S415 Phosphorylation
S419 Phosphorylation
S421 Phosphorylation
K428 Ubiquitination
S451 Phosphorylation
S463 Phosphorylation
S466 Phosphorylation
K480 Sumoylation
K480 Ubiquitination
K485 Sumoylation
K485 Ubiquitination
S498 Phosphorylation
K544 Sumoylation
K544 Ubiquitination
S545 Phosphorylation
S547 Phosphorylation
S554 Phosphorylation
K559 Sumoylation
K559 Ubiquitination
K562 Sumoylation
K562 Ubiquitination
K567 Ubiquitination
K587 Sumoylation
K587 Ubiquitination
S597 Phosphorylation
S602 Phosphorylation
K607 Sumoylation
K607 Ubiquitination
K618 Ubiquitination
S627 Phosphorylation O14757 (CHEK1)
S632 Phosphorylation
K635 Sumoylation
K635 Ubiquitination
K642 Ubiquitination
S653 Phosphorylation
S662 Phosphorylation
S668 Phosphorylation
S677 Phosphorylation P06493 (CDK1)
K680 Acetylation
S681 Phosphorylation
K696 Acetylation
K696 Ubiquitination
K697 Ubiquitination
T700 Phosphorylation
S705 Phosphorylation

研究背景

機能:

Ubiquitin-binding protein. Specifically recognizes and binds 'Lys-63'-linked ubiquitin Ref.37). Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.

PTMs:

Sumoylated.

Phosphorylated upon DNA damage by ATM or ATR.

細胞の位置付け:

Nucleus.
Note: Localizes at sites of DNA damage at double-strand breaks (DSBs).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.

サブユニット構造:

Interacts with TSP57 (By similarity). Component of the ARISC complex, at least composed of UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with ABRAXAS1. Interacts with ESR1, NR6A1 and UBE2I.

タンパク質ファミリー:

The tandem UIM domains form a continuous 60 Angstrom-long alpha-helix and mediate binding to 'Lys-63'-linked ubiquitins. UIM1 and UIM2 bind to the proximal and distal ubiquitin moieties and recognize an 'Ile-44'-centered hydrophobic patch. Since UIMs don't interact with the 'Lys-63' isopeptide bond the UIM-linker region between the 2 UIM domains determines the selectivity for 'Lys-63'-linkage, and its length is very important for specificity.

The Abraxas-interacting region (AIR) mediates the interaction with ABRAXAS1.

Belongs to the RAP80 family.

研究領域

· Genetic Information Processing > Replication and repair > Homologous recombination.

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