Proteasome 20S alpha 5 Antibody - #DF8268
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| 製品: | Proteasome 20S alpha 5 Antibody |
| カタログ: | DF8268 |
| タンパク質の説明: | Rabbit polyclonal antibody to Proteasome 20S alpha 5 |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Chicken, Xenopus |
| 分子量: | 26 kDa; 26kD(Calculated). |
| ユニプロット: | P28066 |
| RRID: | AB_2841557 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8268, RRID:AB_2841557.
折りたたみ／展開
Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; Proteasome (prosome macropain) subunit alpha type 5; Proteasome alpha5 subunit; Proteasome component 5; Proteasome subunit alpha type 5; Proteasome subunit alpha type-5; Proteasome subunit zeta; Proteasome zeta chain; PSA5_HUMAN; PSC5; PSMA5; ZETA;
免疫原
- P28066 PSA5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFLTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGIQTSEGVCLAVEKRITSPLMEPSSIEKIVEIDAHIGCAMSGLIADAKTLIDKARVETQNHWFTYNETMTVESVTQAVSNLALQFGEEDADPGAMSRPFGVALLFGGVDEKGPQLFHMDPSGTFVQCDARAIGSASEGAQSSLQEVYHKSMTLKEAIKSSLIILKQVMEEKLNATNIELATVQPGQNFHMFTKEELEEVIKDI
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P28066 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| Y8 | Phosphorylation | Uniprot | |
| R10 | Methylation | Uniprot | |
| T14 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| R20 | Methylation | Uniprot | |
| Y26 | Phosphorylation | Uniprot | |
| T36 | Phosphorylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| T55 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| K91 | Acetylation | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K149 | Sumoylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| Y185 | Phosphorylation | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| K192 | Sumoylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| K196 | Sumoylation | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| K203 | Sumoylation | Uniprot | |
| K203 | Ubiquitination | Uniprot | |
| K209 | Acetylation | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| T213 | Phosphorylation | Uniprot | |
| T219 | Phosphorylation | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| K239 | Ubiquitination | Uniprot |
研究背景
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
Cytoplasm. Nucleus.
Expressed in fetal brain (at protein level).
The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. PSMA5 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly.
Belongs to the peptidase T1A family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Proteasome.
参考文献
Application: WB Species: rat Sample: gastrocnemius muscle tissue
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