Phospho-JAK1 (Tyr1022)[Tyr1034] Antibody - #AF2012

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製品: Phospho-JAK1 (Tyr1022)[Tyr1034] Antibody
カタログ: AF2012
タンパク質の説明: Rabbit polyclonal antibody to Phospho-JAK1 (Tyr1022)[Tyr1034]
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 130kDa; 133kD(Calculated).
ユニプロット: P23458
RRID: AB_2834437

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:500, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(91%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
クローナリティ:
Polyclonal
特異性:
Phospho-JAK1 (Tyr1022)[Tyr1034] Antibody detects endogenous levels of JAK1 only when phosphorylated at Tyr1034, which site historically referenced as Tyr1022.
RRID:
AB_2834437
引用形式: Affinity Biosciences Cat# AF2012, RRID:AB_2834437.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

JAK 1; JAK 1A; JAK 1B; JAK-1; JAK1; JAK1_HUMAN; JAK1A; JAK1B; Janus kinase 1 (a protein tyrosine kinase); Janus kinase 1; JTK3; Tyrosine protein kinase JAK 1; Tyrosine protein kinase JAK1; Tyrosine-protein kinase JAK1;

免疫原

免疫原:
Uniprot:

P23458(JAK1_HUMAN) >>Visit HPA database.

遺伝子(ID):
JAK1(3716)
発現特異性:
P23458 JAK1_HUMAN:

Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.

タンパク質の説明:
Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain.
タンパク質配列:
MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Horse
91
Xenopus
91
Zebrafish
55
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23458 基板として

Site PTM Type Enzyme
Phosphorylation
M1 Acetylation
Y3 Phosphorylation
K100 Ubiquitination
T119 Phosphorylation
S125 Phosphorylation
K142 Ubiquitination
K213 Ubiquitination
S216 Phosphorylation
Y217 Phosphorylation
Y220 Phosphorylation
K227 Ubiquitination
S228 Phosphorylation
K245 Ubiquitination
K249 Ubiquitination
K254 Ubiquitination
K267 Ubiquitination
K269 Acetylation
K269 Ubiquitination
S333 Phosphorylation
K386 Ubiquitination
Y412 Phosphorylation
K493 Ubiquitination
K496 Ubiquitination
K504 Ubiquitination
Y507 Phosphorylation
S515 Phosphorylation
S518 Phosphorylation
S524 Phosphorylation
K541 Ubiquitination
K559 Ubiquitination
Y568 Phosphorylation
S574 Phosphorylation
K581 Ubiquitination
Y605 Phosphorylation
K606 Ubiquitination
T687 Phosphorylation
T688 Phosphorylation
K691 Ubiquitination
K696 Ubiquitination
K708 Ubiquitination
K718 Ubiquitination
K736 Ubiquitination
S738 Phosphorylation
K768 Ubiquitination
K802 Ubiquitination
K847 Ubiquitination
S857 Phosphorylation
K860 Ubiquitination
K872 Ubiquitination
K888 Ubiquitination
K908 Ubiquitination
S909 Phosphorylation
K911 Ubiquitination
K923 Ubiquitination
K924 Ubiquitination
K939 Ubiquitination
K941 Ubiquitination
Y967 Phosphorylation
K978 Ubiquitination
Y993 Phosphorylation
K1018 Ubiquitination
K1026 Ubiquitination
T1030 Phosphorylation
K1032 Ubiquitination
Y1034 Phosphorylation P52333 (JAK3) , P23458 (JAK1)
Y1035 Phosphorylation P23458 (JAK1) , P52333 (JAK3)
T1036 Phosphorylation
K1038 Ubiquitination
S1043 Phosphorylation
T1107 Phosphorylation
K1109 Acetylation
K1109 Ubiquitination
K1112 Acetylation
Y1125 Phosphorylation
K1130 Ubiquitination

PTMs - P23458 酵素として

Substrate Site Source
P14784 (IL2RB) Y364 Uniprot
P15260 (IFNGR1) Y457 Uniprot
P19525-1 (EIF2AK2) Y101 Uniprot
P19525-1 (EIF2AK2) Y293 Uniprot
P23458 (JAK1) Y1034 Uniprot
P23458 (JAK1) Y1035 Uniprot
P29597 (TYK2) Y1054 Uniprot
P29597 (TYK2) Y1055 Uniprot
P35568 (IRS1) Y612 Uniprot
P35568 (IRS1) Y632 Uniprot
P35568 (IRS1) Y662 Uniprot
P35568 (IRS1) Y732 Uniprot
P40763-2 (STAT3) Y704 Uniprot
P40763 (STAT3) Y705 Uniprot
P42224-2 (STAT1) Y701 Uniprot
P52630-3 (STAT2) Y690 Uniprot
Q13651 (IL10RA) Y446 Uniprot
Q13651 (IL10RA) Y496 Uniprot
Q99683 (MAP3K5) Y718 Uniprot

研究背景

機能:

Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor as well as interleukin (IL)-10 receptor.

PTMs:

Autophosphorylated. Phosphorylated on tyrosine residues in response to interferon gamma signaling. Dephosphorylation of Tyr-1034 and Tyr-1035 by PTPN2 negatively regulates cytokine-mediated signaling.

Ubiquitinated by RNF125; leading to its degradation by the proteasome.

細胞の位置付け:

Endomembrane system>Peripheral membrane protein.
Note: Wholly intracellular, possibly membrane associated.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.

サブユニット構造:

Interacts with IL31RA. Interacts with IFNAR2. Interacts with IFNGR1. Interacts with JAKMIP1. Interacts with SHB. Interacts (via N-terminus) with IL2RB and IL10RA (via its cytoplasmic domain). Interacts with FER (By similarity).

タンパク質ファミリー:

Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1.

The FERM domain mediates interaction with JAKMIP1.

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

研究領域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

参考文献

1). Aligned electrospun poly(l-lactide) nanofibers facilitate wound healing by inhibiting macrophage M1 polarization via the JAK-STAT and NF-κB pathways. JOURNAL OF NANOBIOTECHNOLOGY, 2022 (PubMed: 35883095) [IF=10.2]

Application: WB    Species: Mice    Sample:

Fig. 3 The underlying mechanism by which aligned fibers affected macrophage polarization. A Venn diagram showing differentially expressed genes. B KEGG pathway analysis between the A20 and R20 groups. C Heatmap of differentially expressed genes among the three groups. D Heatmap of macrophage polarization-related genes between the A20 and R20 groups. E Volcano diagram of differentially expressed genes. F Western blot analysis of the NF-κB signaling pathway. G Immunofluorescence staining showing the nuclear translocation of NF-κB p65. The nucleus is stained blue, and NF-κB p65 protein is stained red. H Western blot images and semiquantitative analysis of the JAK-STAT signaling pathway (*p < 0.05, **p < 0.01, n = 3)
2). Graveoline attenuates D-GalN/LPS-induced acute liver injury via inhibition of JAK1/STAT3 signaling pathway. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 39018876) [IF=7.5]
3). A novel fluorinated triazole derivative suppresses macrophage activation and alleviates experimental colitis via a Twist1-dependent pathway. BIOCHEMICAL PHARMACOLOGY, 2018 (PubMed: 30028990) [IF=5.8]
4). Monotropein inhibits colitis associated cancer through VDR/JAK1/STAT1 regulation of macrophage polarization. International Immunopharmacology, 2023 (PubMed: 37633235) [IF=5.6]
5). Irradiation-induced senescence of bone marrow mesenchymal stem cells aggravates osteogenic differentiation dysfunction via paracrine signaling. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2020 (PubMed: 32233952) [IF=5.5]

Application: WB    Species: Mouse    Sample: bone marrow-derived mesenchymal stem cells (BMSCs)

Fig. 6. Involvement of the JAK1/STAT3 pathway and the paracrine effects of irradiation-induced senescent BMSCs on osteoblasts osteogenic differentiation. A-B:Activation of JAK1/STAT3 pathway after irradiation of 10Gy, as shown in western blot analyses of p-JAK1, T-STAT3 and p-STAT3, while 0.8μM JAK1 inhibitor intervention could effectively inhibite JAK1/STAT3 pathway, and the downstream p-STAT3 expression was more significantly blocked. C: ELISA results show the upregulated secretion of IL-6, IL-8 and MMP9 in the supernatant collected 72h after Downloaded from journals.physiology.org/journal/ajpcell at Lunds Univ Medicinska Fak Biblio (130.235.066.010) on April 8, 2020.10Gy-irradiation and suppressed secretion after adding 0.8μM JAK1 inhibitor following irradiation. D-G: Representative images and quantitative analysis of ALP activity and mineralized nodule area of osteoblasts co-cultured with different types of CM from irradiated BMSCs (Con CM, Con/JAKi CM, IRIS CM and IRIS/JAKi CM).Magnification, ×100. H-I: Relative mRNA and protein expression levels of ALP and OC of osteoblasts co-cultured with four types of CM from irradiated BMSCs. All data were analyzed from three independent experiments. P values were calculated by Student’s t-test and one-way ANOVA analysis. Results are presented as mean ± SD.*p < 0.01, **p < 0.01, ***p < 0.001. ALP: alkaline phosphatase; OC: osteocalcin; Con CM: CM from control BMSCs; Con/JAKi CM: CM from control BMSCs with JAKi intervention; IRIS CM: CM from irradiation-induced senescent BMSCs; IRIS/JAKi CM: CM from irradiation-induced senescent BMSCs with JAKi intervention. SD:standard deviation. Scale bar: 100μm (D, F).
6). Transcriptional Regulation of Voltage-Gated Sodium Channels Contributes to GM-CSF-Induced Pain. JOURNAL OF NEUROSCIENCE, 2019 (PubMed: 31015342) [IF=5.3]

Application: WB    Species: rat    Sample: DRG neurons

Figure 5. | GM-CSF increase the mRNA expression level of Nav1.7, Nav1.8, 822 Nav1.9 channel through Jak2-Stat3 signaling pathway. (A) Relative expression of 823 p-Jak1, p-Jak2, p-Jak3, p-stat3 and p-stat5 in DRG neurons after incubation with 824 GM-CSF for 25 mins. (n=3, unpaired t-test, *P < 0.05 as compared to control)
7). BCL2L10 inhibits growth and metastasis of hepatocellular carcinoma both in vitro and in vivo. MOLECULAR CARCINOGENESIS, 2017 (PubMed: 27770580) [IF=4.6]

Application: WB    Species: human    Sample: HepG2

Figure 6. Effect of BCL2L10 on its downstream gene expression profiles of human cancer pathway in HepG2 cells. (A) By human cancer pathway PCR array, ectopic expression of BCL2L10 up- or down-regulated several genes related to tumor proliferation, apoptosis, metastasis and angiogenesis. (B) Western blot was performed to confirm the downstream gene expression regulated by BCL2L10 in HepG2 cells. GAPDH was used as an internal control. (C) Schematic diagram of the molecular events for BCL2L10 function as a tumor suppressor through regulating cell cycle, proliferation, apoptosis metastasis and angiogenesis effectors.
8). Combination of gemcitabine and erlotinib inhibits recurrent pancreatic cancer growth in mice via the JAK-STAT pathway. Oncology Reports, 2018 (PubMed: 29328487) [IF=4.2]

Application: WB    Species: human    Sample: BxPC-3 and PANC‑1 cells

Figure 3.| The gemcitabine-erlotinib (E+G) combination group inhibits the activity of the JAK-STAT pathway, as well as the expression of downstream HIF‑1α, cyclin D1 and p53. (E) Phosphorylation levels of JAK1 (Tyr1022), JAK2 (Tyr221), JAK3(Tyr981) and STAT3 (Tyr701) in BxPC-3 and PANC‑1 cells were analyzed by western blotting, respectively.
9). Curcumin prevents osteocyte apoptosis by inhibiting M1-type macrophage polarization in mice model of glucocorticoid-associated osteonecrosis of the femoral head. Journal of Orthopaedic Research®, 2020 (PubMed: 32009245) [IF=2.8]

Application: WB    Species: Mice    Sample: macrophages

Figure 5 A‐F, The expression of JAK1, p‐JAK1, JAK2, p‐JAK2, STAT1, and p‐STAT1 in macrophages within each group as determined by western blot analysis. G‐I, Quantitative analysis of the bands measured by gray value. The experiment was carried out a minimum of three times, and values are expressed as the mean ± SD. *P  < .05 and **P < .01 compared with MI. JAK, Janus kinase; STAT, signal transducer and activator of transcription [Color figure can be viewed at wileyonlinelibrary.com]
10). Scorpion venom polypeptide governs alveolar macrophage M1/M2 polarization to alleviate pulmonary fibrosis. Tissue and Cell, 2022 (PubMed: 36179453) [IF=2.6]
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