RCC1 Antibody - #DF8275
製品: | RCC1 Antibody |
カタログ: | DF8275 |
タンパク質の説明: | Rabbit polyclonal antibody to RCC1 |
アプリケーション: | WB |
反応性: | Human, Mouse, Rat |
予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
分子量: | 45 kDa; 45kD(Calculated). |
ユニプロット: | P18754 |
RRID: | AB_2841564 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8275, RRID:AB_2841564.
折りたたみ/展開
Cell cycle regulatory protein; CHC 1; CHC1; Chromosome condensation 1; Chromosome condensation protein 1; Guanine nucleotide releasing protein; HERC2; Ran GEF; RanGEF; RCC 1; RCC1; RCC1 I; RCC1_HUMAN; Regulator of chromosome condensation 1; Regulator of chromosome condensation; SHEP1; SNHG3 RCC1; SNHG3 RCC1 readthrough transcript;
免疫原
- P18754 RCC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSPKRIAKRRSPPADAIPKSKKVKVSHRSHSTEPGLVLTLGQGDVGQLGLGENVMERKKPALVSIPEDVVQAEAGGMHTVCLSKSGQVYSFGCNDEGALGRDTSVEGSEMVPGKVELQEKVVQVSAGDSHTAALTDDGRVFLWGSFRDNNGVIGLLEPMKKSMVPVQVQLDVPVVKVASGNDHLVMLTADGDLYTLGCGEQGQLGRVPELFANRGGRQGLERLLVPKCVMLKSRGSRGHVRFQDAFCGAYFTFAISHEGHVYGFGLSNYHQLGTPGTESCFIPQNLTSFKNSTKSWVGFSGGQHHTVCMDSEGKAYSLGRAEYGRLGLGEGAEEKSIPTLISRLPAVSSVACGASVGYAVTKDGRVFAWGMGTNYQLGTGQDEDAWSPVEMMGKQLENRVVLSVSSGGQHTVLLVKDKEQS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P18754 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S2 | Methylation | Uniprot | |
S2 | Phosphorylation | Q13188 (STK3) , P06493 (CDK1) | Uniprot |
S11 | Phosphorylation | P06493 (CDK1) , Q13188 (STK3) | Uniprot |
S20 | Phosphorylation | Uniprot | |
S26 | Phosphorylation | Uniprot | |
S29 | Phosphorylation | Uniprot | |
T32 | Phosphorylation | Uniprot | |
S85 | Phosphorylation | Uniprot | |
Y89 | Phosphorylation | Uniprot | |
S90 | Phosphorylation | Uniprot | |
C93 | S-Nitrosylation | Uniprot | |
S104 | Phosphorylation | Uniprot | |
K114 | Sumoylation | Uniprot | |
K114 | Ubiquitination | Uniprot | |
K120 | Ubiquitination | Uniprot | |
T131 | Phosphorylation | Uniprot | |
T135 | Phosphorylation | Uniprot | |
K160 | Ubiquitination | Uniprot | |
K161 | Ubiquitination | Uniprot | |
S162 | Phosphorylation | Uniprot | |
R214 | Methylation | Uniprot | |
K227 | Ubiquitination | Uniprot | |
K232 | Ubiquitination | Uniprot | |
T274 | Phosphorylation | P06493 (CDK1) | Uniprot |
T306 | Phosphorylation | Uniprot | |
S311 | Phosphorylation | Uniprot | |
K335 | Sumoylation | Uniprot | |
K335 | Ubiquitination | Uniprot | |
S336 | Phosphorylation | Uniprot | |
T339 | Phosphorylation | Uniprot | |
K362 | Ubiquitination | Uniprot | |
S387 | Phosphorylation | P06493 (CDK1) | Uniprot |
S403 | Phosphorylation | Uniprot | |
S405 | Phosphorylation | Uniprot | |
S406 | Phosphorylation | Uniprot | |
T411 | Phosphorylation | Uniprot |
研究背景
Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis. Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis. Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA.
N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di- and trimethylation produce a permanent positive charge on the amino group, which facilitates electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
Nucleus. Chromosome. Cytoplasm.
Note: Predominantly nuclear in interphase cells (PubMed:12194828). Binds to mitotic chromosomes (PubMed:12194828, PubMed:17435751, PubMed:20668449).
Interacts with RAN. Interacts (via N-terminus and RCC1 repeats) with KPNA4. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation.
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