TRPM7 Antibody - #DF8300
(1)
| 製品: | TRPM7 Antibody |
| カタログ: | DF8300 |
| タンパク質の説明: | Rabbit polyclonal antibody to TRPM7 |
| アプリケーション: | WB |
| 反応性: | Human |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 213 kDa; 213kD(Calculated). |
| ユニプロット: | Q96QT4 |
| RRID: | AB_2841583 |
製品説明
ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human
予測:
Pig(100%), Bovine(91%), Horse(82%), Sheep(91%), Rabbit(100%), Dog(91%)
クローナリティ:
Polyclonal
特異性:
TRPM7 Antibody detects endogenous levels of total TRPM7.
RRID:
AB_2841583
引用形式: Affinity Biosciences Cat# DF8300, RRID:AB_2841583.
引用形式: Affinity Biosciences Cat# DF8300, RRID:AB_2841583.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
ALSPDC; CHAK; CHAK1; Channel kinase 1; Channel-kinase 1; Long transient receptor potential channel 7; LTrpC-7; LTrpC7; Transient receptor potential cation channel subfamily M member 7; TRP PLIK; TRPM7; TRPM7_HUMAN;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
- Q96QT4 TRPM7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSQKSWIESTLTKRECVYIIPSSKDPHRCLPGCQICQQLVRCFCGRLVKQHACFTASLAMKYSDVKLGDHFNQAIEEWSVEKHTEQSPTDAYGVINFQGGSHSYRAKYVRLSYDTKPEVILQLLLKEWQMELPKLVISVHGGMQKFELHPRIKQLLGKGLIKAAVTTGAWILTGGVNTGVAKHVGDALKEHASRSSRKICTIGIAPWGVIENRNDLVGRDVVAPYQTLLNPLSKLNVLNNLHSHFILVDDGTVGKYGAEVRLRRELEKTINQQRIHARIGQGVPVVALIFEGGPNVILTVLEYLQESPPVPVVVCEGTGRAADLLAYIHKQTEEGGNLPDAAEPDIISTIKKTFNFGQNEALHLFQTLMECMKRKELITVFHIGSDEHQDIDVAILTALLKGTNASAFDQLILTLAWDRVDIAKNHVFVYGQQWLVGSLEQAMLDALVMDRVAFVKLLIENGVSMHKFLTIPRLEELYNTKQGPTNPMLFHLVRDVKQGNLPPGYKITLIDIGLVIEYLMGGTYRCTYTRKRFRLIYNSLGGNNRRSGRNTSSSTPQLRKSHESFGNRADKKEKMRHNHFIKTAQPYRPKIDTVMEEGKKKRTKDEIVDIDDPETKRFPYPLNELLIWACLMKRQVMARFLWQHGEESMAKALVACKIYRSMAYEAKQSDLVDDTSEELKQYSNDFGQLAVELLEQSFRQDETMAMKLLTYELKNWSNSTCLKLAVSSRLRPFVAHTCTQMLLSDMWMGRLNMRKNSWYKVILSILVPPAILLLEYKTKAEMSHIPQSQDAHQMTMDDSENNFQNITEEIPMEVFKEVRILDSNEGKNEMEIQMKSKKLPITRKFYAFYHAPIVKFWFNTLAYLGFLMLYTFVVLVQMEQLPSVQEWIVIAYIFTYAIEKVREIFMSEAGKVNQKIKVWFSDYFNISDTIAIISFFIGFGLRFGAKWNFANAYDNHVFVAGRLIYCLNIIFWYVRLLDFLAVNQQAGPYVMMIGKMVANMFYIVVIMALVLLSFGVPRKAILYPHEAPSWTLAKDIVFHPYWMIFGEVYAYEIDVCANDSVIPQICGPGTWLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLQVKAISNIVWKYQRYHFIMAYHEKPVLPPPLIILSHIVSLFCCICKRRKKDKTSDGPKLFLTEEDQKKLHDFEEQCVEMYFNEKDDKFHSGSEERIRVTFERVEQMCIQIKEVGDRVNYIKRSLQSLDSQIGHLQDLSALTVDTLKTLTAQKASEASKVHNEITRELSISKHLAQNLIDDGPVRPSVWKKHGVVNTLSSSLPQGDLESNNPFHCNILMKDDKDPQCNIFGQDLPAVPQRKEFNFPEAGSSSGALFPSAVSPPELRQRLHGVELLKIFNKNQKLGSSSTSIPHLSSPPTKFFVSTPSQPSCKSHLETGTKDQETVCSKATEGDNTEFGAFVGHRDSMDLQRFKETSNKIKILSNNNTSENTLKRVSSLAGFTDCHRTSIPVHSKQAEKISRRPSTEDTHEVDSKAALIPDWLQDRPSNREMPSEEGTLNGLTSPFKPAMDTNYYYSAVERNNLMRLSQSIPFTPVPPRGEPVTVYRLEESSPNILNNSMSSWSQLGLCAKIEFLSKEEMGGGLRRAVKVQCTWSEHDILKSGHLYIIKSFLPEVVNTWSSIYKEDTVLHLCLREIQQQRAAQKLTFAFNQMKPKSIPYSPRFLEVFLLYCHSAGQWFAVEECMTGEFRKYNNNNGDEIIPTNTLEEIMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKAEEKRSCDMVFGPANLGEDAIKNFRAKHHCNSCCRKLKLPDLKRNDYTPDKIIFPQDEPSDLNLQPGNSTKESESTNSVRLML
種類予測
種類予測:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Rabbit
100
Pig
100
Bovine
91
Sheep
91
Dog
91
Horse
82
Chicken
73
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96QT4 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| T12 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| T523 | Phosphorylation | Uniprot | |
| S554 | Phosphorylation | Uniprot | |
| T555 | Phosphorylation | Uniprot | |
| K667 | Ubiquitination | Uniprot | |
| S764 | Phosphorylation | Uniprot | |
| Y776 | Phosphorylation | Uniprot | |
| T778 | Phosphorylation | Uniprot | |
| K827 | Ubiquitination | Uniprot | |
| S907 | Phosphorylation | Uniprot | |
| K1159 | Ubiquitination | Uniprot | |
| T1163 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| K1168 | Acetylation | Uniprot | |
| S1191 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1193 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1230 | Phosphorylation | Uniprot | |
| S1239 | Phosphorylation | Uniprot | |
| K1253 | Ubiquitination | Uniprot | |
| S1255 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1258 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1265 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1269 | Phosphorylation | Uniprot | |
| S1271 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1287 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1358 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1361 | Phosphorylation | Uniprot | |
| S1386 | Phosphorylation | Uniprot | |
| S1387 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1390 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1395 | Phosphorylation | Uniprot | |
| S1396 | Phosphorylation | Uniprot | |
| S1404 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1405 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1407 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1410 | Phosphorylation | Uniprot | |
| S1413 | Phosphorylation | Uniprot | |
| T1417 | Phosphorylation | Uniprot | |
| T1419 | Phosphorylation | Uniprot | |
| T1435 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1446 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1455 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1456 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1463 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1468 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1471 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| K1473 | Methylation | Uniprot | |
| K1473 | Ubiquitination | Uniprot | |
| S1476 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1477 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1482 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1488 | Phosphorylation | Uniprot | |
| S1493 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1500 | Phosphorylation | Uniprot | |
| S1504 | Phosphorylation | Uniprot | |
| T1505 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1508 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1513 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1527 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1533 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1537 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1542 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1543 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1551 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1567 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1569 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1583 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1598 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1615 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1660 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1685 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1695 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1779 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1830 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| K1833 | Ubiquitination | Uniprot | |
| S1851 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1860 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
PTMs - Q96QT4 酵素として
| Substrate | Site | Source |
|---|---|---|
| O00418 (EEF2K) | S78 | Uniprot |
| P04083 (ANXA1) | S5 | Uniprot |
| P28289 (TMOD1) | S2 | Uniprot |
| P28289 (TMOD1) | T54 | Uniprot |
| P35579 (MYH9) | T1800 | Uniprot |
| P35579 (MYH9) | S1803 | Uniprot |
| P35579 (MYH9) | S1808 | Uniprot |
| Q96QT4 (TRPM7) | T1163 | Uniprot |
| Q96QT4 (TRPM7) | S1191 | Uniprot |
| Q96QT4 (TRPM7) | S1193 | Uniprot |
| Q96QT4 (TRPM7) | S1255 | Uniprot |
| Q96QT4 (TRPM7) | S1258 | Uniprot |
| Q96QT4 (TRPM7) | T1265 | Uniprot |
| Q96QT4 (TRPM7) | S1271 | Uniprot |
| Q96QT4 (TRPM7) | S1287 | Uniprot |
| Q96QT4 (TRPM7) | S1358 | Uniprot |
| Q96QT4 (TRPM7) | S1387 | Uniprot |
| Q96QT4 (TRPM7) | S1390 | Uniprot |
| Q96QT4 (TRPM7) | S1404 | Uniprot |
| Q96QT4 (TRPM7) | T1405 | Uniprot |
| Q96QT4 (TRPM7) | S1407 | Uniprot |
| Q96QT4 (TRPM7) | T1435 | Uniprot |
| Q96QT4 (TRPM7) | S1446 | Uniprot |
| Q96QT4 (TRPM7) | T1455 | Uniprot |
| Q96QT4 (TRPM7) | S1456 | Uniprot |
| Q96QT4 (TRPM7) | S1463 | Uniprot |
| Q96QT4 (TRPM7) | S1468 | Uniprot |
| Q96QT4 (TRPM7) | T1471 | Uniprot |
| Q96QT4 (TRPM7) | S1476 | Uniprot |
| Q96QT4 (TRPM7) | S1477 | Uniprot |
| Q96QT4 (TRPM7) | T1482 | Uniprot |
| Q96QT4 (TRPM7) | S1493 | Uniprot |
| Q96QT4 (TRPM7) | T1505 | Uniprot |
| Q96QT4 (TRPM7) | T1508 | Uniprot |
| Q96QT4 (TRPM7) | S1513 | Uniprot |
| Q96QT4 (TRPM7) | S1527 | Uniprot |
| Q96QT4 (TRPM7) | S1533 | Uniprot |
| Q96QT4 (TRPM7) | T1537 | Uniprot |
| Q96QT4 (TRPM7) | T1542 | Uniprot |
| Q96QT4 (TRPM7) | S1543 | Uniprot |
| Q96QT4 (TRPM7) | T1551 | Uniprot |
| Q96QT4 (TRPM7) | S1567 | Uniprot |
| Q96QT4 (TRPM7) | S1569 | Uniprot |
| Q96QT4 (TRPM7) | T1583 | Uniprot |
| Q96QT4 (TRPM7) | S1598 | Uniprot |
| Q96QT4 (TRPM7) | S1615 | Uniprot |
| Q96QT4 (TRPM7) | S1660 | Uniprot |
| Q96QT4 (TRPM7) | T1685 | Uniprot |
| Q96QT4 (TRPM7) | S1695 | Uniprot |
| Q96QT4 (TRPM7) | S1779 | Uniprot |
| Q96QT4 (TRPM7) | T1830 | Uniprot |
| Q96QT4 (TRPM7) | S1851 | Uniprot |
| Q96QT4 (TRPM7) | S1860 | Uniprot |
研究背景
機能:
Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. Involved in TNF-induced necroptosis downstream of MLKL by mediating calcium influx. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
PTMs:
Autophosphorylated.
細胞の位置付け:
Membrane>Multi-pass membrane protein.
サブユニット構造:
Homodimer. Interacts with PLCB1 (By similarity). Forms heterodimers with TRPM6.
タンパク質ファミリー:
In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.
In the N-terminal section; belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM7 sub-subfamily.
研究領域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Digestive system > Mineral absorption.
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