PANX1 Antibody - #DF8316

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製品: PANX1 Antibody
カタログ: DF8316
タンパク質の説明: Rabbit polyclonal antibody to PANX1
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
分子量: 48 kDa; 48kD(Calculated).
ユニプロット: Q96RD7
RRID: AB_2841594

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
PANX1 Antibody detects endogenous levels of total PANX1.
RRID:
AB_2841594
引用形式: Affinity Biosciences Cat# DF8316, RRID:AB_2841594.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

innexin; MRS1; Pannexin 1; Pannexin-1; Panx1; PANX1_HUMAN; PX1; UNQ2529;

免疫原

免疫原:
Uniprot:

Q96RD7(PANX1_HUMAN) >>Visit HPA database.

遺伝子(ID):
PANX1(24145)
タンパク質配列:
MAIAQLATEYVFSDFLLKEPTEPKFKGLRLELAVDKMVTCIAVGLPLLLISLAFAQEISIGTQISCFSPSSFSWRQAAFVDSYCWAAVQQKNSLQSESGNLPLWLHKFFPYILLLFAILLYLPPLFWRFAAAPHICSDLKFIMEELDKVYNRAIKAAKSARDLDMRDGACSVPGVTENLGQSLWEVSESHFKYPIVEQYLKTKKNSNNLIIKYISCRLLTLIIILLACIYLGYYFSLSSLSDEFVCSIKSGILRNDSTVPDQFQCKLIAVGIFQLLSVINLVVYVLLAPVVVYTLFVPFRQKTDVLKVYEILPTFDVLHFKSEGYNDLSLYNLFLEENISEVKSYKCLKVLENIKSSGQGIDPMLLLTNLGMIKMDVVDGKTPMSAEMREEQGNQTAELQGMNIDSETKANNGEKNARQRLLDSSC

PTMs - Q96RD7 基板として

Site PTM Type Enzyme
Y10 Phosphorylation
Y150 Phosphorylation
S182 Phosphorylation
S189 Phosphorylation
Y199 Phosphorylation
K201 Ubiquitination
K203 Acetylation
K204 Acetylation
K212 Acetylation
N255 N-Glycosylation
K307 Ubiquitination
K343 Ubiquitination
K355 Ubiquitination
K381 Ubiquitination
S385 Phosphorylation
K409 Ubiquitination
S425 Phosphorylation

研究背景

機能:

Structural component of the gap junctions and the hemichannels. May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis.

PTMs:

S-nitrosylation inhibits channel currents and ATP release.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Cell junction>Gap junction. Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homohexamer. Forms homomeric or PANX1/PANX2-heteromeric intercellular channels on coexpression in paired Xenopus oocytes (By similarity).

タンパク質ファミリー:

Belongs to the pannexin family.

研究領域

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

参考文献

1). Processing of angiocrine alarmin IL-1α in endothelial cells promotes lung and liver fibrosis. International immunopharmacology, 2024 (PubMed: 38723369) [IF=5.6]
2). Carbenoxolone has the potential to ameliorate acute incision pain in rats. Molecular Medicine Reports, 2021 (PubMed: 34013377) [IF=3.4]

Application: WB    Species: Rat    Sample:

Figure 2. Px1 and Cx43 expression in the spinal cords of IP model rats. Expression of Px1 and Cx42 in rats was detected via western blotting at (A) 2, (B) 4, (C) 6 and (D) 24 h post-surgery. The scanning density of (E) Px1 and (F) Cx43 expression normalized to control. *P<0.05, **P<0.01 and ***P<0.001 vs. C group; #P<0.05, ##P<0.01 and ###P<0.001 vs. NS group; ΔP<0.05, ΔΔP<0.01 and ΔΔΔP<0.001 vs. CBX group. Data are presented as the mean ± SD. C, control group; IP, incision pain; NS, normal saline; CBX, carbenoxolone; 10panx, pannexin-1 mimetic inhibitory peptide; Px1, pannexin 1; Cx43, connexin 43.

Application: IF/ICC    Species: Rat    Sample:

Figure 3. Co-localization of Px1 and GFAP in IP model rats. Px1 (green) and GFAP (red) expression was analyzed using an immunofluorescence assay. Magnification, ×200; scale bar, 10 µm. C, control group; IP, incision pain; CBX, carbenoxolone; 10panx, pannexin-1 mimetic inhibitory peptide; Px1, pannexin 1; GFAP, glial fibrillary acidic protein.

Application: IF/ICC    Species: rat    Sample:

Figure 3. |Co‑localization of Px1 and GFAP in IP model rats. Px1 (green) and GFAP (red)expression was analyzed using an immunofluorescence assay.Magnification, x200; scale bar, 10 µm. C,control group; IP, incision pain; CBX, carbenoxolone; 10panx, pannexin‑1 mimetic inhibitory peptide; Px1, pannexin 1;GFAP, glial fibrillary acidic protein

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