MYH11 Antibody - #DF8344
製品: | MYH11 Antibody |
カタログ: | DF8344 |
タンパク質の説明: | Rabbit polyclonal antibody to MYH11 |
アプリケーション: | WB IHC |
反応性: | Human, Mouse, Rat |
予測: | Bovine, Horse, Rabbit, Dog |
分子量: | 227 kDa; 227kD(Calculated). |
ユニプロット: | P35749 |
RRID: | AB_2841611 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8344, RRID:AB_2841611.
折りたたみ/展開
AAT4; DKFZp686D10126; DKFZp686D19237; FAA4; FLJ35232; MGC126726; MGC32963; MYH 11; MYH11; MYH11_HUMAN; Myosin 11; Myosin heavy chain 11; Myosin heavy chain 11 smooth muscle; Myosin heavy chain; Myosin heavy chain smooth muscle isoform; Myosin heavy polypeptide 11 smooth muscle; Myosin-11; SMHC; SMMHC; smooth muscle isoform; Smooth muscle myosin heavy chain 11 isoform SM2; Smooth muscle myosin heavy chain isoform SM2;
免疫原
Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea. Isoform 1 is mostly found in slowly contracting tonic muscles.
- P35749 MYH11_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQKGQLSDDEKFLFVDKNFINSPVAQADWAAKRLVWVPSEKQGFEAASIKEEKGDEVVVELVENGKKVTVGKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYFSGLIYTYSGLFCVVVNPYKHLPIYSEKIVDMYKGKKRHEMPPHIYAIADTAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDTSITGELEKQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAIRQARDERTFHIFYYMIAGAKEKMRSDLLLEGFNNYTFLSNGFVPIPAAQDDEMFQETVEAMAIMGFSEEEQLSILKVVSSVLQLGNIVFKKERNTDQASMPDNTAAQKVCHLMGINVTDFTRSILTPRIKVGRDVVQKAQTKEQADFAVEALAKATYERLFRWILTRVNKALDKTHRQGASFLGILDIAGFEIFEVNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPNNPPGVLALLDEECWFPKATDKSFVEKLCTEQGSHPKFQKPKQLKDKTEFSIIHYAGKVDYNASAWLTKNMDPLNDNVTSLLNASSDKFVADLWKDVDRIVGLDQMAKMTESSLPSASKTKKGMFRTVGQLYKEQLGKLMTTLRNTTPNFVRCIIPNHEKRSGKLDAFLVLEQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILAANAIPKGFMDGKQACILMIKALELDPNLYRIGQSKIFFRTGVLAHLEEERDLKITDVIMAFQAMCRGYLARKAFAKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTKVKPLLQVTRQEEEMQAKEDELQKTKERQQKAENELKELEQKHSQLTEEKNLLQEQLQAETELYAEAEEMRVRLAAKKQELEEILHEMEARLEEEEDRGQQLQAERKKMAQQMLDLEEQLEEEEAARQKLQLEKVTAEAKIKKLEDEILVMDDQNNKLSKERKLLEERISDLTTNLAEEEEKAKNLTKLKNKHESMISELEVRLKKEEKSRQELEKLKRKLEGDASDFHEQIADLQAQIAELKMQLAKKEEELQAALARLDDEIAQKNNALKKIRELEGHISDLQEDLDSERAARNKAEKQKRDLGEELEALKTELEDTLDSTATQQELRAKREQEVTVLKKALDEETRSHEAQVQEMRQKHAQAVEELTEQLEQFKRAKANLDKNKQTLEKENADLAGELRVLGQAKQEVEHKKKKLEAQVQELQSKCSDGERARAELNDKVHKLQNEVESVTGMLNEAEGKAIKLAKDVASLSSQLQDTQELLQEETRQKLNVSTKLRQLEEERNSLQDQLDEEMEAKQNLERHISTLNIQLSDSKKKLQDFASTVEALEEGKKRFQKEIENLTQQYEEKAAAYDKLEKTKNRLQQELDDLVVDLDNQRQLVSNLEKKQRKFDQLLAEEKNISSKYADERDRAEAEAREKETKALSLARALEEALEAKEELERTNKMLKAEMEDLVSSKDDVGKNVHELEKSKRALETQMEEMKTQLEELEDELQATEDAKLRLEVNMQALKGQFERDLQARDEQNEEKRRQLQRQLHEYETELEDERKQRALAAAAKKKLEGDLKDLELQADSAIKGREEAIKQLRKLQAQMKDFQRELEDARASRDEIFATAKENEKKAKSLEADLMQLQEDLAAAERARKQADLEKEELAEELASSLSGRNALQDEKRRLEARIAQLEEELEEEQGNMEAMSDRVRKATQQAEQLSNELATERSTAQKNESARQQLERQNKELRSKLHEMEGAVKSKFKSTIAALEAKIAQLEEQVEQEAREKQAATKSLKQKDKKLKEILLQVEDERKMAEQYKEQAEKGNARVKQLKRQLEEAEEESQRINANRRKLQRELDEATESNEAMGREVNALKSKLRRGNETSFVPSRRSGGRRVIENADGSEEETDTRDADFNGTKASE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P35749 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S8 | Phosphorylation | Uniprot | |
S23 | Phosphorylation | Uniprot | |
K67 | Acetylation | Uniprot | |
K78 | Acetylation | Uniprot | |
K83 | Ubiquitination | Uniprot | |
S85 | Phosphorylation | Uniprot | |
K129 | Methylation | Uniprot | |
Y142 | Phosphorylation | Uniprot | |
K143 | Acetylation | Uniprot | |
K146 | Acetylation | Uniprot | |
Y155 | Phosphorylation | Uniprot | |
Y162 | Phosphorylation | Uniprot | |
S173 | Phosphorylation | Uniprot | |
K184 | Ubiquitination | Uniprot | |
Y194 | Phosphorylation | Uniprot | |
T208 | Phosphorylation | Uniprot | |
K232 | Ubiquitination | Uniprot | |
T233 | Phosphorylation | Uniprot | |
K235 | Ubiquitination | Uniprot | |
K244 | Ubiquitination | Uniprot | |
K268 | Ubiquitination | Uniprot | |
K362 | Acetylation | Uniprot | |
K363 | Acetylation | Uniprot | |
T367 | Phosphorylation | Uniprot | |
S371 | Phosphorylation | Uniprot | |
T376 | Phosphorylation | Uniprot | |
S395 | Phosphorylation | Uniprot | |
K547 | Ubiquitination | Uniprot | |
S548 | Phosphorylation | Uniprot | |
S559 | Phosphorylation | Uniprot | |
T604 | Phosphorylation | Uniprot | |
S637 | Phosphorylation | Uniprot | |
S638 | Phosphorylation | Uniprot | |
T652 | Phosphorylation | Uniprot | |
K658 | Methylation | Uniprot | |
T666 | Phosphorylation | Uniprot | |
T667 | Phosphorylation | Uniprot | |
R718 | Methylation | Uniprot | |
Y761 | Phosphorylation | Uniprot | |
S766 | Phosphorylation | Uniprot | |
K767 | Acetylation | Uniprot | |
K767 | Ubiquitination | Uniprot | |
Y826 | Phosphorylation | Uniprot | |
K828 | Acetylation | Uniprot | |
K828 | Ubiquitination | Uniprot | |
K842 | Ubiquitination | Uniprot | |
S883 | Phosphorylation | Uniprot | |
T900 | Phosphorylation | Uniprot | |
Y903 | Phosphorylation | Uniprot | |
K968 | Ubiquitination | Uniprot | |
S1009 | Phosphorylation | Uniprot | |
S1034 | Phosphorylation | Uniprot | |
K1088 | Acetylation | Uniprot | |
K1088 | Ubiquitination | Uniprot | |
K1111 | Acetylation | Uniprot | |
S1121 | Phosphorylation | Uniprot | |
T1153 | Phosphorylation | Uniprot | |
S1161 | Phosphorylation | Uniprot | |
T1162 | Phosphorylation | Uniprot | |
S1189 | Phosphorylation | Uniprot | |
K1216 | Acetylation | Uniprot | |
K1226 | Ubiquitination | Uniprot | |
S1266 | Phosphorylation | Uniprot | |
T1336 | Phosphorylation | Uniprot | |
S1347 | Phosphorylation | Uniprot | |
S1367 | Phosphorylation | Uniprot | |
T1368 | Phosphorylation | Uniprot | |
K1377 | Acetylation | Uniprot | |
K1378 | Acetylation | Uniprot | |
K1395 | Acetylation | Uniprot | |
T1405 | Phosphorylation | Uniprot | |
Y1408 | Phosphorylation | Uniprot | |
Y1415 | Phosphorylation | Uniprot | |
T1421 | Phosphorylation | Uniprot | |
S1444 | Phosphorylation | Uniprot | |
K1448 | Acetylation | Uniprot | |
K1449 | Acetylation | Uniprot | |
K1452 | Acetylation | Uniprot | |
K1452 | Methylation | Uniprot | |
K1452 | Ubiquitination | Uniprot | |
S1465 | Phosphorylation | Uniprot | |
Y1467 | Phosphorylation | Uniprot | |
K1484 | Acetylation | Uniprot | |
K1484 | Ubiquitination | Uniprot | |
S1487 | Phosphorylation | Uniprot | |
T1558 | Phosphorylation | Uniprot | |
K1562 | Ubiquitination | Uniprot | |
Y1601 | Phosphorylation | Uniprot | |
T1603 | Phosphorylation | Uniprot | |
K1619 | Acetylation | Uniprot | |
K1649 | Ubiquitination | Uniprot | |
S1667 | Phosphorylation | Uniprot | |
T1674 | Phosphorylation | Uniprot | |
K1680 | Acetylation | Uniprot | |
K1681 | Acetylation | Uniprot | |
R1701 | Methylation | Uniprot | |
K1731 | Acetylation | Uniprot | |
S1810 | Phosphorylation | Uniprot | |
K1811 | Acetylation | Uniprot | |
K1813 | Acetylation | Uniprot | |
S1814 | Phosphorylation | Uniprot | |
T1815 | Phosphorylation | Uniprot | |
Y1868 | Phosphorylation | Uniprot | |
K1925 | Methylation | Uniprot | |
S1954 | Phosphorylation | Uniprot | |
T1958 | Phosphorylation | Uniprot | |
T1960 | Phosphorylation | Uniprot |
研究背景
Muscle contraction.
Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea. Isoform 1 is mostly found in slowly contracting tonic muscles.
Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
参考文献
Application: WB Species: Rat Sample: VSMCs
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