UBA3 Antibody - #DF8358
| 製品: | UBA3 Antibody |
| カタログ: | DF8358 |
| タンパク質の説明: | Rabbit polyclonal antibody to UBA3 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 49 kDa; 52kD(Calculated). |
| ユニプロット: | Q8TBC4 |
| RRID: | AB_2841623 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8358, RRID:AB_2841623.
折りたたみ／展開
DKFZp566J164; EC 6.3.2.; hUba3; MGC22384; NEDD8 activating enzyme E1 catalytic subunit; NEDD8 activating enzyme E1C; Nedd8 activating enzyme hUba3; NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1C; uba3; UBA3 ubiquitin activating enzyme E1 homolog; UBA3_HUMAN; UBE1C; Ubiquitin activating enzyme 3; Ubiquitin activating enzyme E1C; Ubiquitin-activating enzyme 3; Ubiquitin-activating enzyme E1C; Ubiquitin-like modifier-activating enzyme 3;
免疫原
- Q8TBC4 UBA3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADGEEPEKKRRRIEELLAEKMAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8TBC4 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K9 | Methylation | Uniprot | |
| K10 | Methylation | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| T103 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| R135 | Methylation | Uniprot | |
| K147 | Sumoylation | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| Y185 | Phosphorylation | Uniprot | |
| T203 | Phosphorylation | Uniprot | |
| K207 | Sumoylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| K284 | Sumoylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| R295 | Methylation | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| K361 | Ubiquitination | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| T390 | Phosphorylation | Uniprot | |
| S392 | Phosphorylation | Uniprot | |
| S394 | Phosphorylation | Uniprot | |
| K398 | Ubiquitination | Uniprot | |
| S399 | Phosphorylation | Uniprot | |
| K409 | Acetylation | Uniprot | |
| K409 | Ubiquitination | Uniprot | |
| K434 | Ubiquitination | Uniprot |
研究背景
Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.
Ubiquitously expressed.
Heterodimer of UBA3 and NAE1. Interacts with NEDD8, UBE2F and UBE2M. Binds ESR1 and ESR2 with bound steroid ligand (By similarity). Interacts with TBATA (By similarity).
Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
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