METAP2 Antibody - #DF8406
| 製品: | METAP2 Antibody |
| カタログ: | DF8406 |
| タンパク質の説明: | Rabbit polyclonal antibody to METAP2 |
| アプリケーション: | WB |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 52 kDa; 53kD(Calculated). |
| ユニプロット: | P50579 |
| RRID: | AB_2841657 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8406, RRID:AB_2841657.
折りたたみ／展開
A930035J23Rik; AI047573; AL024412; Amp2; AU014659,; EIF 2 associated p67 homolog; EIF-2-associated p67; Initiation factor 2 associated 67 kDa glycoprotein; Initiation factor 2-associated 67 kDa glycoprotein; MAP 2; MAP2; MAP2_HUMAN; MetAP 2; Metap2; Methionine aminopeptidase 2; Methionyl aminopeptidase 2; MGC102452; MGC127390; MGC53792; MNPEP; p67; p67eIF2; Peptidase M; Peptidase M2;
免疫原
- P50579 MAP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSAAGEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKKKKKKKRGPKVQTDPPSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGDDY
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P50579 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| R23 | Methylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| T30 | Phosphorylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| T96 | Phosphorylation | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| Y137 | Phosphorylation | Uniprot | |
| T145 | Phosphorylation | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| Y182 | Phosphorylation | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| K323 | Ubiquitination | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| T365 | Phosphorylation | Uniprot | |
| T369 | Phosphorylation | Uniprot | |
| K385 | Ubiquitination | Uniprot | |
| K427 | Acetylation | Uniprot | |
| K427 | Ubiquitination | Uniprot | |
| C436 | S-Nitrosylation | Uniprot | |
| C448 | S-Nitrosylation | Uniprot |
研究背景
Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding.
Cytoplasm.
Note: About 30% of expressed METAP2 associates with polysomes.
Interacts strongly with the eIF-2 gamma-subunit EIF2S3 (By similarity). Binds EIF2S1 at low magnesium concentrations.
Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.
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