ENC1 Antibody - #DF8407
| 製品: | ENC1 Antibody |
| カタログ: | DF8407 |
| タンパク質の説明: | Rabbit polyclonal antibody to ENC1 |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 66 kDa; 66kD(Calculated). |
| ユニプロット: | O14682 |
| RRID: | AB_2841658 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8407, RRID:AB_2841658.
折りたたみ／展開
CCL 28; CCL28; Ectoderm neural cortex protein 1; Ectoderm-neural cortex protein 1; Ectodermal neural cortex (with BTB like domain); ENC 1; ENC-1; ENC1; ENC1_HUMAN; FLJ39259; Kelch like 35; Kelch like 37; Kelch-like protein 37; KLHL 35; KLHL35; KLHL37; NRPB; Nuclear matrix protein NRP/B; Nuclear restricted protein BTB domain like (brain); OTTHUMP00000128235; OTTHUMP00000222054; p53 induced gene 10 protein; p53-induced gene 10 protein; PIG 10; PIG10; TP53I10; Tumor protein p53 inducible protein 10;
免疫原
Detected in fetal brain tissue, moderate expression in fetal heart, lung and kidney. Highly expressed in adult brain, particularly high in the hippocampus and amygdala, and spinal chord. Detectable in adult pancreas. May be down-regulated in neuroblastoma tumors.
- O14682 ENC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSVSVHENRKSRASSGSINIYLFHKSSYADSVLTHLNLLRQQRLFTDVLLHAGNRTFPCHRAVLAACSRYFEAMFSGGLKESQDSEVNFDNSIHPEVLELLLDYAYSSRVIINEENAESLLEAGDMLEFQDIRDACAEFLEKNLHPTNCLGMLLLSDAHQCTKLYELSWRMCLSNFQTIRKNEDFLQLPQDMVVQLLSSEELETEDERLVYESAINWISYDLKKRYCYLPELLQTVRLALLPAIYLMENVAMEELITKQRKSKEIVEEAIRCKLKILQNDGVVTSLCARPRKTGHALFLLGGQTFMCDKLYLVDQKAKEIIPKADIPSPRKEFSACAIGCKVYITGGRGSENGVSKDVWVYDTLHEEWSKAAPMLVARFGHGSAELKHCLYVVGGHTAATGCLPASPSVSLKQVEHYDPTINKWTMVAPLREGVSNAAVVSAKLKLFAFGGTSVSHDKLPKVQCYDQCENRWTVPATCPQPWRYTAAAVLGNQIFIMGGDTEFSACSAYKFNSETYQWTKVGDVTAKRMSCHAVASGNKLYVVGGYFGIQRCKTLDCYDPTLDVWNSITTVPYSLIPTAFVSTWKHLPS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O14682 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| T46 | Phosphorylation | Uniprot | |
| Y70 | Phosphorylation | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| S406 | Phosphorylation | Uniprot | |
| Y417 | Phosphorylation | Uniprot | |
| K423 | Ubiquitination | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| S441 | Phosphorylation | Uniprot | |
| K443 | Ubiquitination | Uniprot | |
| T452 | Phosphorylation | Uniprot | |
| S453 | Phosphorylation | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| K520 | Ubiquitination | Uniprot | |
| K527 | Ubiquitination | Uniprot | |
| T569 | Phosphorylation | Uniprot | |
| T570 | Phosphorylation | Uniprot | |
| Y573 | Phosphorylation | Uniprot |
研究背景
Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. Down-regulates transcription factor NF2L2/NRF2 by decreasing the rate of protein synthesis and not via a ubiquitin-mediated proteasomal degradation mechanism.
Ubiquitinated by E3 ubiquitin ligase complex formed by CUL3 and RBX1 and probably targeted for proteasome-independent degradation. Quinone-induced oxidative stress increases its ubiquitination.
Nucleus matrix. Cytoplasm. Cytoplasm>Cytoskeleton.
Detected in fetal brain tissue, moderate expression in fetal heart, lung and kidney. Highly expressed in adult brain, particularly high in the hippocampus and amygdala, and spinal chord. Detectable in adult pancreas. May be down-regulated in neuroblastoma tumors.
Binds to RB1. Hypophosphorylated RB1 associates with ENC1 during neuronal differentiation, while hyperphosphorylated RB1 associates with ENC1 in undifferentiating cells. Part of a complex that contains CUL3, RBX1 and ENC1. Interacts indirectly with KEAP1.
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