SRD5A2 Antibody - #DF8416

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製品: SRD5A2 Antibody
カタログ: DF8416
タンパク質の説明: Rabbit polyclonal antibody to SRD5A2
アプリケーション: WB IHC
反応性: Human
予測: Pig, Bovine, Horse, Sheep
分子量: 28 kDa; 28kD(Calculated).
ユニプロット: P31213
RRID: AB_2841665

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MS Report
HPLC Report
MSDS
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(89%), Bovine(89%), Horse(89%), Sheep(89%)
クローナリティ:
Polyclonal
特異性:
SRD5A2 Antibody detects endogenous levels of total SRD5A2.
RRID:
AB_2841665
引用形式: Affinity Biosciences Cat# DF8416, RRID:AB_2841665.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

3 oxo 5 alpha steroid 4 dehydrogenase 2; 3-oxo-5-alpha-steroid 4-dehydrogenase 2; 5 alpha SR2; 5 alpha-SR2; 5ART2; e II 5 alpha reductase; EC; MGC138457; Micropenis, included; S5A2_HUMAN; S5AR 2; SR type 2; SRD5A2; Steroid 5 alpha reductase 2; Steroid 5-alpha-reductase 2; Steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2); Type II 5-alpha reductase;

免疫原

免疫原:
Uniprot:

P31213(S5A2_HUMAN) >>Visit HPA database.

遺伝子(ID):
SRD5A2(6716)
発現特異性:
P31213 S5A2_HUMAN:

Expressed in high levels in the prostate and many other androgen-sensitive tissues.

タンパク質配列:
MQVQCQQSPVLAGSATLVALGALALYVAKPSGYGKHTESLKPAATRLPARAAWFLQELPSFAVPAGILARQPLSLFGPPGTVLLGLFCVHYFHRTFVYSLLNRGRPYPAILILRGTAFCTGNGVLQGYYLIYCAEYPDGWYTDIRFSLGVFLFILGMGINIHSDYILRQLRKPGEISYRIPQGGLFTYVSGANFLGEIIEWIGYALATWSLPALAFAFFSLCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
89
Horse
89
Bovine
89
Sheep
89
Dog
78
Rabbit
67
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31213 基板として

Site PTM Type Enzyme
Y91 Phosphorylation
Y98 Phosphorylation
Y165 Phosphorylation
S177 Phosphorylation

研究背景

機能:

Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.

細胞の位置付け:

Microsome membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in high levels in the prostate and many other androgen-sensitive tissues.

タンパク質ファミリー:

Belongs to the steroid 5-alpha reductase family.

研究領域

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

参考文献

1). Risk subtyping and prognostic assessment of prostate cancer based on consensus genes. Communications Biology, 2022 (PubMed: 35293897) [IF=5.9]

Application: IHC    Species: Human    Sample: PCa tumor tissues

Fig. 4 Immunohistochemistry validation of SRD5A2 and ITGA11 expression in PCa tumor tissues. a Representative pictures showing the different protein levels of SRD5A2 in prostate cancer patients with different Gleason scores. Quantitative comparisons of H-scores were also performed in Gleason and pathological tumor stage subgroups. b Representative pictures showing the different protein levels of ITGA11 in prostate cancer patients with different Gleason scores. Quantitative comparison of staining density was also performed in Gleason and pathological tumor stage subgroups. Data are presented as the mean ± SD by t-test, bars represent mean values, error bars represent SD. Scale bars, 50 μm, 200 μm.

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