GHR Antibody - #DF8425
(2)
(3)
| 製品: | GHR Antibody |
| カタログ: | DF8425 |
| タンパク質の説明: | Rabbit polyclonal antibody to GHR |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 140 kDa, 80 kDa; 72kD(Calculated). |
| ユニプロット: | P10912 |
| RRID: | AB_2841673 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8425, RRID:AB_2841673.
折りたたみ／展開
GH receptor; GH-binding protein; GHBP; GHBP, included; GHR; GHR_HUMAN; Growth hormone binding protein; Growth hormone receptor; Growth hormone receptor precursor; Growth hormone-binding protein; Growth hormone-binding protein, included; Increased responsiveness to growth hormone, included; Serum binding protein; Serum-binding protein; Somatotropin receptor;
免疫原
Expressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression in placenta is predominant in chorion and decidua. Isoform 4 is highly expressed in placental villi. Isoform 2 is expressed in lung, stomach and muscle. Low levels in liver.
- P10912 GHR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDLWQLLLTLALAGSSDAFSGSEATAAILSRAPWSLQSVNPGLKTNSSKEPKFTKCRSPERETFSCHWTDEVHHGTKNLGPIQLFYTRRNTQEWTQEWKECPDYVSAGENSCYFNSSFTSIWIPYCIKLTSNGGTVDEKCFSVDEIVQPDPPIALNWTLLNVSLTGIHADIQVRWEAPRNADIQKGWMVLEYELQYKEVNETKWKMMDPILTTSVPVYSLKVDKEYEVRVRSKQRNSGNYGEFSEVLYVTLPQMSQFTCEEDFYFPWLLIIIFGIFGLTVMLFVFLFSKQQRIKMLILPPVPVPKIKGIDPDLLKEGKLEEVNTILAIHDSYKPEFHSDDSWVEFIELDIDEPDEKTEESDTDRLLSSDHEKSHSNLGVKDGDSGRTSCCEPDILETDFNANDIHEGTSEVAQPQRLKGEADLLCLDQKNQNNSPYHDACPATQQPSVIQAEKNKPQPLPTEGAESTHQAAHIQLSNPSSLSNIDFYAQVSDITPAGSVVLSPGQKNKAGMSQCDMHPEMVSLCQENFLMDNAYFCEADAKKCIPVAPHIKVESHIQPSLNQEDIYITTESLTTAAGRPGTGEHVPGSEMPVPDYTSIHIVQSPQGLILNATALPLPDKEFLSSCGYVSTDQLNKIMP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P10912 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T76 | Phosphorylation | Uniprot | |
| Y218 | Phosphorylation | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| Y226 | Phosphorylation | Uniprot | |
| Y332 | Phosphorylation | O60674 (JAK2) | Uniprot |
| S341 | Phosphorylation | Uniprot | |
| T357 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| Y487 | Phosphorylation | O60674 (JAK2) | Uniprot |
| Y534 | Phosphorylation | Uniprot | |
| Y566 | Phosphorylation | Uniprot | |
| Y595 | Phosphorylation | Uniprot | |
| Y627 | Phosphorylation | Uniprot | |
| S629 | Phosphorylation | Uniprot |
研究背景
Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway (By similarity).
The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.
Isoform 2 up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling.
The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17 (By similarity).
On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2.
On ligand binding, ubiquitinated on lysine residues in the cytoplasmic domain. This ubiquitination is not sufficient for GHR internalization (By similarity).
Cell membrane>Single-pass type I membrane protein.
Note: On growth hormone binding, GHR is ubiquitinated, internalized, down-regulated and transported into a degradative or non-degradative pathway.
Cell membrane>Single-pass type I membrane protein.
Note: Remains fixed to the cell membrane and is not internalized.
Secreted.
Note: Complexed to a substantial fraction of circulating GH.
Expressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression in placenta is predominant in chorion and decidua. Isoform 4 is highly expressed in placental villi. Isoform 2 is expressed in lung, stomach and muscle. Low levels in liver.
On growth hormone (GH) binding, forms homodimers and binds JAK2 via a box 1-containing domain. Binding to SOCS3 inhibits JAK2 activation, binding to CIS and SOCS2 inhibits STAT5 activation. Interacts with ADAM17.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
The extracellular domain is the ligand-binding domain representing the growth hormone-binding protein (GHBP).
The ubiquitination-dependent endocytosis motif (UbE) is required for recruitment of the ubiquitin conjugation system on to the receptor and for its internalization.
Belongs to the type I cytokine receptor family. Type 1 subfamily.
研究領域
· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
参考文献
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