ATP7A Antibody - #DF8506

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製品: ATP7A Antibody
カタログ: DF8506
タンパク質の説明: Rabbit polyclonal antibody to ATP7A
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 163 kDa; 163kD(Calculated).
ユニプロット: Q04656
RRID: AB_2841712

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(87%), Horse(93%), Sheep(87%), Rabbit(100%), Dog(93%)
クローナリティ:
Polyclonal
特異性:
ATP7A Antibody detects endogenous levels of total ATP7A.
RRID:
AB_2841712
引用形式: Affinity Biosciences Cat# DF8506, RRID:AB_2841712.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ATP 7A; ATP7A; ATP7A_HUMAN; ATPase copper transporting alpha polypeptide; ATPase Cu++ transporting alpha polypeptide (Menkes syndrome); ATPase Cu++ transporting alpha polypeptide; Copper pump 1; Copper transporting ATPase 1; Copper-transporting ATPase 1; Cu++ transporting P type ATPase; DSMAX; FLJ17790; MC 1; MC1; Menkes disease associated protein; Menkes disease-associated protein; Menkes syndrome; MK; MNK; OHS; OTTHUMP00000062077; SMAX3;

免疫原

免疫原:
Uniprot:

Q04656(ATP7A_HUMAN) >>Visit HPA database.

遺伝子(ID):
ATP7A(538)
発現特異性:
Q04656 ATP7A_HUMAN:

Found in most tissues except liver. Isoform 3 is widely expressed including in liver cell lines. Isoform 1 is expressed in fibroblasts, choriocarcinoma, colon carcinoma and neuroblastoma cell lines. Isoform 2 is expressed in fibroblasts, colon carcinoma and neuroblastoma cell lines.

タンパク質配列:
MDPSMGVNSVTISVEGMTCNSCVWTIEQQIGKVNGVHHIKVSLEEKNATIIYDPKLQTPKTLQEAIDDMGFDAVIHNPDPLPVLTDTLFLTVTASLTLPWDHIQSTLLKTKGVTDIKIYPQKRTVAVTIIPSIVNANQIKELVPELSLDTGTLEKKSGACEDHSMAQAGEVVLKMKVEGMTCHSCTSTIEGKIGKLQGVQRIKVSLDNQEATIVYQPHLISVEEMKKQIEAMGFPAFVKKQPKYLKLGAIDVERLKNTPVKSSEGSQQRSPSYTNDSTATFIIDGMHCKSCVSNIESTLSALQYVSSIVVSLENRSAIVKYNASSVTPESLRKAIEAVSPGLYRVSITSEVESTSNSPSSSSLQKIPLNVVSQPLTQETVINIDGMTCNSCVQSIEGVISKKPGVKSIRVSLANSNGTVEYDPLLTSPETLRGAIEDMGFDATLSDTNEPLVVIAQPSSEMPLLTSTNEFYTKGMTPVQDKEEGKNSSKCYIQVTGMTCASCVANIERNLRREEGIYSILVALMAGKAEVRYNPAVIQPPMIAEFIRELGFGATVIENADEGDGVLELVVRGMTCASCVHKIESSLTKHRGILYCSVALATNKAHIKYDPEIIGPRDIIHTIESLGFEASLVKKDRSASHLDHKREIRQWRRSFLVSLFFCIPVMGLMIYMMVMDHHFATLHHNQNMSKEEMINLHSSMFLERQILPGLSVMNLLSFLLCVPVQFFGGWYFYIQAYKALKHKTANMDVLIVLATTIAFAYSLIILLVAMYERAKVNPITFFDTPPMLFVFIALGRWLEHIAKGKTSEALAKLISLQATEATIVTLDSDNILLSEEQVDVELVQRGDIIKVVPGGKFPVDGRVIEGHSMVDESLITGEAMPVAKKPGSTVIAGSINQNGSLLICATHVGADTTLSQIVKLVEEAQTSKAPIQQFADKLSGYFVPFIVFVSIATLLVWIVIGFLNFEIVETYFPGYNRSISRTETIIRFAFQASITVLCIACPCSLGLATPTAVMVGTGVGAQNGILIKGGEPLEMAHKVKVVVFDKTGTITHGTPVVNQVKVLTESNRISHHKILAIVGTAESNSEHPLGTAITKYCKQELDTETLGTCIDFQVVPGCGISCKVTNIEGLLHKNNWNIEDNNIKNASLVQIDASNEQSSTSSSMIIDAQISNALNAQQYKVLIGNREWMIRNGLVINNDVNDFMTEHERKGRTAVLVAVDDELCGLIAIADTVKPEAELAIHILKSMGLEVVLMTGDNSKTARSIASQVGITKVFAEVLPSHKVAKVKQLQEEGKRVAMVGDGINDSPALAMANVGIAIGTGTDVAIEAADVVLIRNDLLDVVASIDLSRKTVKRIRINFVFALIYNLVGIPIAAGVFMPIGLVLQPWMGSAAMAASSVSVVLSSLFLKLYRKPTYESYELPARSQIGQKSPSEISVHVGIDDTSRNSPKLGLLDRIVNYSRASINSLLSDKRSLNSVVTSEPDKHSLLVGDFREDDDTAL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
93
Dog
93
Bovine
87
Sheep
87
Zebrafish
64
Xenopus
53
Pig
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q04656 基板として

Site PTM Type Enzyme
S147 Phosphorylation
T152 Phosphorylation
K155 Ubiquitination
K195 Ubiquitination
K226 Acetylation
K227 Acetylation
K239 Acetylation
Y244 Phosphorylation
K246 Ubiquitination
S266 Phosphorylation
S270 Phosphorylation
S272 Phosphorylation
Y273 Phosphorylation
T278 Phosphorylation
Y321 Phosphorylation
S325 Phosphorylation
T327 Phosphorylation
S330 Phosphorylation
K333 Ubiquitination
S339 Phosphorylation
Y343 Phosphorylation
S346 Phosphorylation
S349 Phosphorylation
S353 Phosphorylation
T354 Phosphorylation
S355 Phosphorylation
S357 Phosphorylation
S359 Phosphorylation
S361 Phosphorylation
S362 Phosphorylation
Y471 Phosphorylation
T476 Phosphorylation
Y517 Phosphorylation
S518 Phosphorylation
Y594 Phosphorylation
T601 Phosphorylation
K633 Ubiquitination
S827 Phosphorylation
K849 Ubiquitination
K855 Ubiquitination
K1037 Ubiquitination
K1039 Ubiquitination
K1045 Ubiquitination
K1060 Ubiquitination
K1132 Ubiquitination
T1204 Phosphorylation
T1212 Phosphorylation
S1306 Phosphorylation
K1412 Ubiquitination
S1424 Phosphorylation
S1430 Phosphorylation
S1432 Phosphorylation
S1435 Phosphorylation
S1444 Phosphorylation
S1447 Phosphorylation
K1449 Ubiquitination
Y1459 Phosphorylation
S1460 Phosphorylation
S1463 Phosphorylation
S1466 Phosphorylation
S1469 Phosphorylation
K1471 Ubiquitination
S1473 Phosphorylation
S1476 Phosphorylation
T1479 Phosphorylation
S1480 Phosphorylation
S1486 Phosphorylation
T1498 Phosphorylation

研究背景

機能:

May supply copper to copper-requiring proteins within the secretory pathway, when localized in the trans-Golgi network. Under conditions of elevated extracellular copper, it relocalized to the plasma membrane where it functions in the efflux of copper from cells.

細胞の位置付け:

Golgi apparatus>trans-Golgi network membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.
Note: Cycles constitutively between the trans-Golgi network (TGN) and the plasma membrane (PubMed:9147644). Predominantly found in the TGN and relocalized to the plasma membrane in response to elevated copper levels.

Cytoplasm>Cytosol.

Endoplasmic reticulum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Found in most tissues except liver. Isoform 3 is widely expressed including in liver cell lines. Isoform 1 is expressed in fibroblasts, choriocarcinoma, colon carcinoma and neuroblastoma cell lines. Isoform 2 is expressed in fibroblasts, colon carcinoma and neuroblastoma cell lines.

サブユニット構造:

Monomer. Interacts with PDZD11. Interacts with ATOX1 and COMMD1.

タンパク質ファミリー:

The C-terminal di-leucine, 1487-Leu-Leu-1488, is an endocytic targeting signal which functions in retrieving recycling from the plasma membrane to the TGN. Mutation of the di-leucine signal results in the accumulation of the protein in the plasma membrane.

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.

研究領域

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Organismal Systems > Digestive system > Mineral absorption.

参考文献

1). Metformin-based carbon dots based on biguanide functional groups for simultaneous chelation of copper ions and inhibitable colorectal cancer therapy. Carbon, 2023 [IF=10.9]
2). Copper transporter gene ATP7A: A predictive biomarker for immunotherapy and targeted therapy in hepatocellular carcinoma. International immunopharmacology, 2023 (PubMed: 36502594) [IF=5.6]

Application: IHC    Species: Human    Sample: HCC tissues

Fig. 5. ATP7A is associated with CD3 + T cells and CD8 + T cells in HCC. (A) Immunohistochemical analysis of ATP7A and CD3, CD8 expression in HCC tissues. (B-E) Association between ATP7A expression and CD3, CD8 expression in HCC patients. ***, P < 0.001.
3). Response of Fibroblasts from Menkes' and Wilson's Copper Metabolism-Related Disorders to Ionizing Radiation: Influence of the Nucleo-Shuttling of the ATM Protein Kinase. Biomolecules, 2023 (PubMed: 38136617) [IF=5.5]

Application: WB    Species: Human    Sample: fibroblasts

Figure 7 Expression of the ATP7A and ATP7B proteins in MD and WD fibroblasts. Anti-ATP7A and anti-ATP7B immunoblots with total or cytoplasmic protein extracts were applied to the indicated non-irradiated control Hs27, MD (A,B), and WD (C,D) fibroblasts. The grey levels corresponding to each condition are shown in Figure S4. Original western blot images can be found in raw data.

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