EAAT2 Antibody - #DF8535
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| 製品: | EAAT2 Antibody |
| カタログ: | DF8535 |
| タンパク質の説明: | Rabbit polyclonal antibody to EAAT2 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 62 kDa; 62kD(Calculated). |
| ユニプロット: | P43004 |
| RRID: | AB_2841739 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8535, RRID:AB_2841739.
折りたたみ／展開
EAA2_HUMAN; EAAT2; Excitatory amino acid transporter 2; Excitotoxic amino acid transporter 2; Glial high affinity glutamate transporter; GLT 1; GLT1; Glutamate aspartate transporter II; Glutamate transporter 1; Glutamate/aspartate transporter II; Slc1a2; Sodium dependent glutamate aspartate transporter 2; Sodium-dependent glutamate/aspartate transporter 2; solute carrier family 1 (glial high affinity glutamate transporter), member 2; Solute carrier family 1 glial high affinity glutamate transporter member 2; Solute carrier family 1 member 2;
免疫原
- P43004 EAA2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASTEGANNMPKQVEVRMHDSHLGSEEPKHRHLGLRLCDKLGKNLLLTLTVFGVILGAVCGGLLRLASPIHPDVVMLIAFPGDILMRMLKMLILPLIISSLITGLSGLDAKASGRLGTRAMVYYMSTTIIAAVLGVILVLAIHPGNPKLKKQLGPGKKNDEVSSLDAFLDLIRNLFPENLVQACFQQIQTVTKKVLVAPPPDEEANATSAVVSLLNETVTEVPEETKMVIKKGLEFKDGMNVLGLIGFFIAFGIAMGKMGDQAKLMVDFFNILNEIVMKLVIMIMWYSPLGIACLICGKIIAIKDLEVVARQLGMYMVTVIIGLIIHGGIFLPLIYFVVTRKNPFSFFAGIFQAWITALGTASSAGTLPVTFRCLEENLGIDKRVTRFVLPVGATINMDGTALYEAVAAIFIAQMNGVVLDGGQIVTVSLTATLASVGAASIPSAGLVTMLLILTAVGLPTEDISLLVAVDWLLDRMRTSVNVVGDSFGAGIVYHLSKSELDTIDSQHRVHEDIEMTKTQSIYDDMKNHRESNSNQCVYAAHNSVIVDECKVTLAANGKSADCSVEEEPWKREK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P43004 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | Uniprot | |
| T4 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| Y123 | Phosphorylation | Uniprot | |
| Y124 | Phosphorylation | Uniprot | |
| Y523 | Phosphorylation | Uniprot | |
| K571 | Sumoylation | Uniprot |
研究背景
Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate (By similarity).
Glycosylated.
Palmitoylation at Cys-38 is not required for correct subcellular localization, but is important for glutamate uptake activity.
Cell membrane>Multi-pass membrane protein.
Homotrimer. Isoform 3 can oligomerize with isoform 1. Interacts with AJUBA (By similarity).
Contains eight transmembrane regions plus two helical hairpins that dip into the membrane. These helical hairpin structures play an important role in the transport process. The first enters the membrane from the cytoplasmic side, the second one from the extracellular side. During the transport cycle, the regions involved in amino acid transport, and especially the helical hairpins, move vertically by about 15-18 Angstroms, alternating between exposure to the aqueous phase and reinsertion in the lipid bilayer. In contrast, the regions involved in trimerization do not move.
Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A2 subfamily.
研究領域
· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).
· Organismal Systems > Nervous system > Glutamatergic synapse.
参考文献
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