製品: ENT1 Antibody
カタログ: DF8542
タンパク質の説明: Rabbit polyclonal antibody to ENT1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
分子量: 50 kDa; 50kD(Calculated).
ユニプロット: Q99808
RRID: AB_2841746

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
ENT1 Antibody detects endogenous levels of total ENT1.
RRID:
AB_2841746
引用形式: Affinity Biosciences Cat# DF8542, RRID:AB_2841746.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Equilibrative NBMPR-sensitive nucleoside transporter; equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; Equilibrative nucleoside transporter 1; es-type; MGC1465; MGC3778; Nucleoside transporter; Nucleoside transporter, es-type; OTTHUMP00000016506; OTTHUMP00000016507; OTTHUMP00000016508; OTTHUMP00000016509; OTTHUMP00000016510; OTTHUMP00000016511; OTTHUMP00000016512; S29A1_HUMAN; Slc29a1; solute carrier family 29 (equilibrative nucleoside transporter), member 1; solute carrier family 29 (nucleoside transporters), member 1; Solute carrier family 29 member 1;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q99808 S29A1_HUMAN:

Detected in erythrocytes (at protein level). Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.

タンパク質配列:
MTTSHQPQDRYKAVWLIFFMLGLGTLLPWNFFMTATQYFTNRLDMSQNVSLVTAELSKDAQASAAPAAPLPERNSLSAIFNNVMTLCAMLPLLLFTYLNSFLHQRIPQSVRILGSLVAILLVFLITAILVKVQLDALPFFVITMIKIVLINSFGAILQGSLFGLAGLLPASYTAPIMSGQGLAGFFASVAMICAIASGSELSESAFGYFITACAVIILTIICYLGLPRLEFYRYYQQLKLEGPGEQETKLDLISKGEEPRAGKEESGVSVSNSQPTNESHSIKAILKNISVLAFSVCFIFTITIGMFPAVTVEVKSSIAGSSTWERYFIPVSCFLTFNIFDWLGRSLTAVFMWPGKDSRWLPSLVLARLVFVPLLLLCNIKPRRYLTVVFEHDAWFIFFMAAFAFSNGYLASLCMCFGPKKVKPAEAETAGAIMAFFLCLGLALGAVFSFLFRAIV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Chicken
100
Rabbit
100
Pig
90
Xenopus
50
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99808 基板として

Site PTM Type Enzyme
N48 N-Glycosylation
S63 O-Glycosylation
S63 Phosphorylation
T143 Phosphorylation
K239 Ubiquitination
T248 Phosphorylation
K249 Ubiquitination
S254 Phosphorylation
K255 Ubiquitination
K263 Ubiquitination
S266 Phosphorylation
S269 Phosphorylation
S271 Phosphorylation
S273 Phosphorylation
S281 Phosphorylation Q05655 (PRKCD)
K283 Ubiquitination
K287 Ubiquitination
K356 Ubiquitination
K381 Ubiquitination

研究背景

機能:

Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).

PTMs:

Glycosylated.

細胞の位置付け:

Basolateral cell membrane>Multi-pass membrane protein. Apical cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.
Note: Predominantly localized in the basolateral membrane in polarized MDCK cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in erythrocytes (at protein level). Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.

サブユニット構造:

Identified in a complex with STOM.

タンパク質ファミリー:

Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.

研究領域

· Human Diseases > Substance dependence > Alcoholism.

参考文献

1). e5NT inhibitor protects acute restraint stress‐induced depression by regulating nucleoside release in mice. JOURNAL OF PHARMACY AND PHARMACOLOGY, 2020 (PubMed: 32743802) [IF=3.3]

Application: WB    Species: mouse    Sample: hippocampus

Figure 2 |Effects of the expression changes in the expression of nucleoside-metabolizing enzyme induced by ARS in the hippocampus of mice.There were no significant changes in the expression of CNT1, CNT2 (a), ENT1 and ENT2 (b).

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