GnRH I Antibody - #DF8553

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製品: GnRH I Antibody
カタログ: DF8553
タンパク質の説明: Rabbit polyclonal antibody to GnRH I
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Monkey, Chicken, Xenopus
分子量: 10 kDa; 10kD(Calculated).
ユニプロット: P01148
RRID: AB_2841757

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Monkey(%), Chicken(83%), Xenopus(92%)
クローナリティ:
Polyclonal
特異性:
GnRH I Antibody detects endogenous levels of total GnRH I.
RRID:
AB_2841757
引用形式: Affinity Biosciences Cat# DF8553, RRID:AB_2841757.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

GnRH associated peptide 1; GNRH1; Gonadotrophin releasing hormone 1; GRH; HH12; LHRH; LNRH; Luliberin I; Luteinizing releasing hormone; Lutenizing hormome releasing hormone; Progonadoliberin 1; Progonadoliberin I; Prolactin release inhibiting factor;

免疫原

免疫原:
Uniprot:

P01148(GON1_HUMAN) >>Visit HPA database.

遺伝子(ID):
GNRH1(2796)
タンパク質配列:
MKPIQKLLAGLILLTWCVEGCSSQHWSYGLRPGGKRDAENLIDSFQEIVKEVGQLAETQRFECTTHQPRSPLRDLKGALESLIEEETGQKKI

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Chicken
83
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the GnRH family.

参考文献

1). The mechanism of Oatp1a5-mediated microcystin-leucine arginine entering into GnRH neurons. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2019 (PubMed: 31526925) [IF=6.8]

Application: WB    Species: mouse    Sample: GT1-7cells

Fig. 4. | N-linked glycosylation is involved in the process of Oatp1a5 transporting MC-LR. GT1-7 cells were pretreated with N-linked glycosylation inhibitor tunicamycin at a concentration of 0.01 μg/mL for 1 h, and followed by the treatment with MC-LR at a concentration of 500 nM for 24 h. (A) The presence of MC-LR in the cells was measured by immunofluorescence assay (scale bar = 50 μm). (B) Intracellular uptake of MC-LR by the transfected cells were detected by western blotting using an anti-MC-LR antibody. (C) The expression of GnRH was examined by western blotting.
2). Microcystin-leucine arginine induced the apoptosis of GnRH neurons by activating the endoplasmic reticulum stress resulting in a decrease of serum testosterone level in mice. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2021 (PubMed: 33396074) [IF=6.8]

Application: IHC    Species: mice    Sample: Brain

Fig. 2. MC-LR induced apoptosis of GnRH neurons. (A) Apoptotic cells in the hypothalamus of mice were detected by TUNEL test. The apoptotic cells with brown stained nuclei were stained by DAB, and apoptotic index was calculated based on the percentage of TUNEL positive cells in an image (scale bar = 50 µm, n = 6 mice/group, **P < 0.01 vs. the control). (B) A confocal fluorescence microscope was used to detect colocalization of Alexa Fluor 594-labled GPR54 (red), Alexa Fluor 488-labled active-caspase3 (green) and DAPI (blue) (scale bar = 50 µm, n = 3). The marker protein of GnRH neurons is GPR54.(For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
3). Aspartame Intake Delayed Puberty Onset in Female Offspring Rats and Girls. Molecular nutrition & food research, 2024 (PubMed: 38389198) [IF=5.2]
4). OPN5 Regulating Mechanism of Follicle Development Through the TSH-DIO2/DIO3 Pathway in Mountain Ducks Under Different Photoperiods. Frontiers in Physiology, 2022 (PubMed: 35733985) [IF=4.0]
5). Change on apoptosis, autophagy and mitochondria of the Harderian gland in Cricetulus barabensis during age. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2021 (PubMed: 33340652) [IF=2.2]

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