GRO gamma Antibody - #DF8554

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製品: GRO gamma Antibody
カタログ: DF8554
タンパク質の説明: Rabbit polyclonal antibody to GRO gamma
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
分子量: 11 kDa; 11kD(Calculated).
ユニプロット: P19876
RRID: AB_2841758

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
GRO gamma Antibody detects endogenous levels of total GRO gamma.
RRID:
AB_2841758
引用形式: Affinity Biosciences Cat# DF8554, RRID:AB_2841758.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

C-X-C motif chemokine 3; C-X-C motif chemokine ligand 3; Chemokine (C X C motif) ligand 3; Chemokine (CXC motif) ligand 3; Cinc 2; CINC 2b; Cinc2; CINC2b; CXCL 3; Cxcl3; CXCL3_HUMAN; Cytokine induced neutrophil chemoattractant 2; Dcip1; Dendritic cell inflammatory protein 1; Gm1960; GRO protein gamma; GRO-gamma; GRO-gamma(1-73); GRO-gamma(5-73); GRO3; GRO3 oncogene; GROG; Growth regulated protein gamma; Growth-regulated protein gamma; Macrophage inflammatory protein 2 beta precursor; Macrophage inflammatory protein 2-beta; Melanoma growth stimulatory activity gamma; Member 3; MGSA gamma; MIP 2b; MIP2-beta; MIP2B; SCYB3; Small inducible cytokine subfamily B;

免疫原

免疫原:
Uniprot:

P19876(CXCL3_HUMAN) >>Visit HPA database.

遺伝子(ID):
CXCL3(2921)
タンパク質配列:
MAHATLSAAPSNPRLLRVALLLLLLVAASRRAAGASVVTELRCQCLQTLQGIHLKNIQSVNVRSPGPHCAQTEVIATLKNGKKACLNPASPMVQKIIEKILNKGSTN

研究背景

機能:

Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes.

PTMs:

N-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the intercrine alpha (chemokine CxC) family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

参考文献

1). Single-cell transcriptomic analysis reveals that the APP-CD74 axis promotes immunosuppression and progression of testicular tumors. The Journal of pathology, 2024 (PubMed: 39161125) [IF=7.3]

Application: IF/ICC    Species: Human    Sample:

Figure 4 Characterization of myeloid cells in the seminoma testes. (A) t-SNE plots for seven distinct myeloid cell subclusters. (B) Dot plot showing the expression of the top five marker genes in each myeloid cell subcluster. (C) Violin plots showing the expression level of selected marker genes across the clusters. (D) Multiplex immunofluorescence staining for CD1C, CLEC9A, LAMP3, and DAPI in seminoma testes (scale bar: 100 μm). (E) Heatmap of the expression of the maturation, regulatory, migration, and Toll-like receptor (TLR)-related marker genes in each cluster. (F) Bubble plots showing the probability of ligand–receptor pairs between tumor cell cluster 1/3 and three types of dendritic cell subclusters. (G) Heatmap of the expression of the M1, M2, angiogenesis, and phagocytosis-related marker genes in two types of macrophages. (H) Multiplex immunofluorescence staining for SELENOP, CXCL3, and DAPI in seminoma testes (scale bar: 100 μm).
2). Transcriptomics Reveals Effect of Pulsatilla Decoction Butanol Extract in Alleviating Vulvovaginal Candidiasis by Inhibiting Neutrophil Chemotaxis and Activation via TLR4 Signaling. Pharmaceuticals (Basel, Switzerland), 2024 (PubMed: 38794163) [IF=4.6]
3). HDAC6-MYCN-CXCL3 axis mediates allergic inflammation and is necessary for allergic inflammation-promoted cellular interactions. Molecular immunology, 2024 (PubMed: 38176167) [IF=3.6]
4). Upregulation of ITGB6 in primary palmar hyperhidrosis. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 2023 (PubMed: 37212774) [IF=2.1]

Application: WB    Species: Human    Sample:

Fig. 6. Regulatory effects of integrin β6 (ITGB6) on CXCL3, CXCL5, CXCL10, CXCL11, and WNT2 expression levels in sweat gland cells. Sweat gland cells extracted from patients with primary palmar hyperhidrosis were treated with blank control or transfected with overexpression negative control (OE NC) or ITGB6 OE. The CXCL3, CXCL5, CXCL10, CXCL11, and WNT2 (A) mRNA (ITGB6 OE compared to control, Tukey’s honest significant difference (HSD), p < 0.001, all), and (B,C) protein expression (ITGB6 OE compared to control, CXCL3, CXCL5, and CXCL11: Tukey’s HSD, all p < 0.001; CXCL10: Tukey’s HSD, p = 0.004; WNT2: Tukey’s HSD, p = 0.027). The data are presented as mean ± 95% confidence interval (95% CI)

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