IDH2 Antibody - #DF8561
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製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8561, RRID:AB_2841765.
折りたたみ／展開
D2HGA2; ICD-M; IDH; IDH2; IDHM; IDHP_HUMAN; IDP; IDPM; Isocitrate dehydrogenase [NADP], mitochondrial; Isocitrate dehydrogenase 2 (NADP+), mitochondrial; mNADP-IDH; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase;
免疫原
- P48735 IDHP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGYLRVVRSLCRASGSRPAWAPAALTAPTSQEQPRRHYADKRIKVAKPVVEMDGDEMTRIIWQFIKEKLILPHVDIQLKYFDLGLPNRDQTDDQVTIDSALATQKYSVAVKCATITPDEARVEEFKLKKMWKSPNGTIRNILGGTVFREPIICKNIPRLVPGWTKPITIGRHAHGDQYKATDFVADRAGTFKMVFTPKDGSGVKEWEVYNFPAGGVGMGMYNTDESISGFAHSCFQYAIQKKWPLYMSTKNTILKAYDGRFKDIFQEIFDKHYKTDFDKNKIWYEHRLIDDMVAQVLKSSGGFVWACKNYDGDVQSDILAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHYREHQKGRPTSTNPIASIFAWTRGLEHRGKLDGNQDLIRFAQMLEKVCVETVESGAMTKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDRALGRQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P48735 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K48 | Acetylation | Uniprot | |
| K67 | Acetylation | Uniprot | |
| K69 | Acetylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K80 | Acetylation | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| T97 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| K106 | Acetylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| Y107 | Phosphorylation | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| T115 | Phosphorylation | Uniprot | |
| T117 | Phosphorylation | Uniprot | |
| K127 | Acetylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K133 | Acetylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| T146 | Phosphorylation | Uniprot | |
| K155 | Acetylation | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| K166 | Acetylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| Y179 | Phosphorylation | Uniprot | |
| K180 | Acetylation | Uniprot | |
| K180 | Ubiquitination | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| R188 | Methylation | Uniprot | |
| T191 | Phosphorylation | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| T197 | Phosphorylation | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| K242 | Acetylation | Uniprot | |
| Y247 | Phosphorylation | Uniprot | |
| K256 | Acetylation | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| K263 | Acetylation | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| K272 | Acetylation | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| K275 | Acetylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K280 | Acetylation | Uniprot | |
| K282 | Acetylation | Uniprot | |
| K282 | Ubiquitination | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| C308 | S-Nitrosylation | Uniprot | |
| T350 | Phosphorylation | Uniprot | |
| K384 | Acetylation | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| K413 | Acetylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| K442 | Acetylation | Uniprot | |
| K442 | Ubiquitination | Uniprot |
研究背景
Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.
Mitochondrion.
Homodimer.
Belongs to the isocitrate and isopropylmalate dehydrogenases family.
研究領域
· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)
· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).
· Metabolism > Metabolism of other amino acids > Glutathione metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
参考文献
Application: WB Species: Human Sample: HepG2 cells
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