IL4R/CD124 Antibody - #DF8567

(1)   (2)  
製品: IL4R/CD124 Antibody
カタログ: DF8567
タンパク質の説明: Rabbit polyclonal antibody to IL4R/CD124
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse
予測: Horse, Dog
分子量: 89 kDa; 90kD(Calculated).
ユニプロット: P24394
RRID: AB_2841771

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IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Horse(100%), Dog(88%)
クローナリティ:
Polyclonal
特異性:
IL4R/CD124 Antibody detects endogenous levels of total IL4R/CD124.
RRID:
AB_2841771
引用形式: Affinity Biosciences Cat# DF8567, RRID:AB_2841771.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CD124; IL 4R alpha; IL-4 receptor subunit alpha; IL-4-binding protein; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; IL4-BP; Il4r; IL4R nirs variant 1; IL4RA; IL4RA_HUMAN; Interleukin 4 receptor alpha chain; Interleukin 4 receptor; sIL4Ralpha/prot; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; Soluble interleukin-4 receptor subunit alpha;

免疫原

免疫原:
Uniprot:

P24394(IL4RA_HUMAN) >>Visit HPA database.

遺伝子(ID):
IL4R(3566)
発現特異性:
P24394 IL4RA_HUMAN:

Isoform 1 and isoform 2 are highly expressed in activated T-cells.

タンパク質配列:
MGWLCSGLLFPVSCLVLLQVASSGNMKVLQEPTCVSDYMSISTCEWKMNGPTNCSTELRLLYQLVFLLSEAHTCIPENNGGAGCVCHLLMDDVVSADNYTLDLWAGQQLLWKGSFKPSEHVKPRAPGNLTVHTNVSDTLLLTWSNPYPPDNYLYNHLTYAVNIWSENDPADFRIYNVTYLEPSLRIAASTLKSGISYRARVRAWAQCYNTTWSEWSPSTKWHNSYREPFEQHLLLGVSVSCIVILAVCLLCYVSITKIKKEWWDQIPNPARSRLVAIIIQDAQGSQWEKRSRGQEPAKCPHWKNCLTKLLPCFLEHNMKRDEDPHKAAKEMPFQGSGKSAWCPVEISKTVLWPESISVVRCVELFEAPVECEEEEEVEEEKGSFCASPESSRDDFQEGREGIVARLTESLFLDLLGEENGGFCQQDMGESCLLPPSGSTSAHMPWDEFPSAGPKEAPPWGKEQPLHLEPSPPASPTQSPDNLTCTETPLVIAGNPAYRSFSNSLSQSPCPRELGPDPLLARHLEEVEPEMPCVPQLSEPTTVPQPEPETWEQILRRNVLQHGAAAAPVSAPTSGYQEFVHAVEQGGTQASAVVGLGPPGEAGYKAFSSLLASSAVSPEKCGFGASSGEEGYKPFQDLIPGCPGDPAPVPVPLFTFGLDREPPRSPQSSHLPSSSPEHLGLEPGEKVEDMPKPPLPQEQATDPLVDSLGSGIVYSALTCHLCGHLKQCHGQEDGGQTPVMASPCCGCCCGDRSSPPTTPLRAPDPSPGGVPLEASLCPASLAPSGISEKSKSSSSFHPAPGNAQSSSQTPKIVNFVSVGPTYMRVS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
88
Rabbit
63
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P24394 基板として

Site PTM Type Enzyme
K192 Ubiquitination
S383 Phosphorylation
S387 Phosphorylation
S390 Phosphorylation
S391 Phosphorylation
S470 Phosphorylation
S474 Phosphorylation
T476 Phosphorylation
Y497 Phosphorylation
Y575 Phosphorylation
Y603 Phosphorylation
S616 Phosphorylation
Y631 Phosphorylation
Y713 Phosphorylation
S753 Phosphorylation
T756 Phosphorylation
S791 Phosphorylation
T820 Phosphorylation
Y821 Phosphorylation

研究背景

機能:

Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.

Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.

PTMs:

On IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction.

The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase.

細胞の位置付け:

Cell membrane>Single-pass type I membrane protein.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Isoform 1 and isoform 2 are highly expressed in activated T-cells.

サブユニット構造:

The functional IL4 receptor is formed by initial binding of IL4 to IL4R. Subsequent recruitment to the complex of the common gamma chain, in immune cells, creates a type I receptor and, in non-immune cells, of IL13RA1 forms a type II receptor. IL4R can also interact with the IL13/IL13RA1 complex to form a similar type II receptor. Interacts with PIK3C3 (By similarity). Interacts with the SH2-containing phosphatases, PTPN6/SHIP1, PTPN11/SHIP2 and INPP5D/SHIP. Interacts with JAK1 through a Box 1-containing region; inhibited by SOCS5. Interacts with SOCS5; inhibits IL4 signaling (By similarity). Interacts with JAK3. Interacts with CLM1 (By similarity).

タンパク質ファミリー:

The extracellular domain represents the IL4 binding protein (IL4BP).

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

Belongs to the type I cytokine receptor family. Type 4 subfamily.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

参考文献

1). Constructing a better binding peptide for drug delivery targeting the interleukin-4 receptor. JOURNAL OF DRUG TARGETING, 2020 (PubMed: 32363946) [IF=4.5]

Application: WB    Species: human    Sample: tumor cell

Figure 3.| ILBP-b is a better IL-4R-targeted peptide than ILBP-a. (A) Expression of IL-4Rα in different tumor cell lines. GAPDH was used as an internal control. And gray value analysis was performed on each lane in the Western Blot results map using ImageJ software. The obtained values were used as the numerical results of IL-4Rα relative expression levels in the manner of IL-4Rα/GAPDH. The error bars represent the mean ± SD, N=3.**p<0.01, ***p <0.001, compared with SMMC group.

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