製品: Phospho-Dab1 (Tyr232) Antibody
カタログ: AF3029
タンパク質の説明: Rabbit polyclonal antibody to Phospho-Dab1 (Tyr232)
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 60kDa; 64kD(Calculated).
ユニプロット: O75553
RRID: AB_2834459

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
クローナリティ:
Polyclonal
特異性:
Phospho-Dab1 (Tyr232) Antibody detects endogenous levels of Dab1 only when phosphorylated at Tyrosine 232.
RRID:
AB_2834459
引用形式: Affinity Biosciences Cat# AF3029, RRID:AB_2834459.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Dab 1; Dab reelin signal transducer 1; Dab, reelin signal transducer, homolog 1 (Drosophila); Dab1; DAB1_HUMAN; Disabled homolog 1; Disabled homolog 1 Drosophila; Scm; Scr; Scrambler; Yot; Yotari;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
O75553 DAB1_HUMAN:

Mainly expressed in brain.

タンパク質の説明:
DAB1 an adaptor molecule functioning in neural development. The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer.
タンパク質配列:
MSTETELQVAVKTSAKKDSRKKGQDRSEATLIKRFKGEGVRYKAKLIGIDEVSAARGDKLCQDSMMKLKGVVAGARSKGEHKQKIFLTISFGGIKIFDEKTGALQHHHAVHEISYIAKDITDHRAFGYVCGKEGNHRFVAIKTAQAAEPVILDLRDLFQLIYELKQREELEKKAQKDKQCEQAVYQTILEEDVEDPVYQYIVFEAGHEPIRDPETEENIYQVPTSQKKEGVYDVPKSQPVSNGYSFEDFEERFAAATPNRNLPTDFDEIFEATKAVTQLELFGDMSTPPDITSPPTPATPGDAFIPSSSQTLPASADVFSSVPFGTAAVPSGYVAMGAVLPSFWGQQPLVQQQMVMGAQPPVAQVMPGAQPIAWGQPGLFPATQQPWPTVAGQFPPAAFMPTQTVMPLPAAMFQGPLTPLATVPGTSDSTRSSPQTDKPRQKMGKETFKDFQMAQPPPVPSRKPDQPSLTCTSEAFSSYFNKVGVAQDTDDCDDFDISQLNLTPVTSTTPSTNSPPTPAPRQSSPSKSSASHASDPTTDDIFEEGFESPSKSEEQEAPDGSQASSNSDPFGEPSGEPSGDNISPQAGS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
91
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75553 基板として

Site PTM Type Enzyme
K43 Ubiquitination
K45 Ubiquitination
K84 Sumoylation
Y198 Phosphorylation
Y220 Phosphorylation
Y232 Phosphorylation
S433 Phosphorylation
T506 Phosphorylation
S524 Phosphorylation P24941 (CDK2)
S526 Phosphorylation
S548 Phosphorylation

研究背景

機能:

Adapter molecule functioning in neural development. May regulate SIAH1 activity.

PTMs:

Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons. Also phosphorylated on Ser-524 independently of reelin signaling.

組織特異性:

Mainly expressed in brain.

サブユニット構造:

Associates with the SH2 domains of SRC, FYN and ABL (By similarity). Interacts (phosphorylated on tyrosine residues) with CRK and CRKL (via respective SH2 domain) (By similarity). Interacts with SIAH1, LRP8 and VLDLR (By similarity). Interacts with LRP1. Interacts with APLP1 (via NPXY motif) (By similarity). Interacts with DAB2IP (By similarity).

タンパク質ファミリー:

The PID domain specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins.

参考文献

1). Ephrin‑b3 modulates hippocampal neurogenesis and the reelin signaling pathway in a pilocarpine‑induced model of epilepsy. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2018 (PubMed: 29512697) [IF=5.4]

Application: WB    Species: rat    Sample: hippocampus

Figure 3. |Reelin pathway expression in the hippocampus of rats during the development of SE.(E) Western blot analysis for p‑Dab1. β‑actin was used as an endogenous control. (F) Quantification of western blot results of p‑Dab1, presented as the p‑Dab1/Dab1 ratio. Data are presented as the mean ± standard deviation. ***P<0.0001, **P<0.001 by two‑way analysis of variance with Tukey's post hoc test (n=3 rats/per group). Ep, epilepsy; Con, control; Dab1, disabled 1; p‑Dab, phosphorylated Dab1.

Application: IHC    Species: rat    Sample: hippocampus

Figure 5. |Effect of EphB3‑Fc on reelin pathway expression in the hippocampus post‑SE.(C) Representative immunohistochemical staining of p‑Dab1 following EphB3‑Fc/Fc‑control treatment. Left: representative immunohistochemical staining of p‑Dab1 (scale bar=50 µm); right: high‑magnification bright‑field images and the arrows indicate the immunopositive cells (scale bar=10 µm).

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