SLC22A6 Antibody - #DF8582

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製品: SLC22A6 Antibody
カタログ: DF8582
タンパク質の説明: Rabbit polyclonal antibody to SLC22A6
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Horse, Sheep, Rabbit, Dog
分子量: 61 kDa; 62kD(Calculated).
ユニプロット: Q4U2R8
RRID: AB_2841786

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MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Horse(88%), Sheep(88%), Rabbit(100%), Dog(88%)
クローナリティ:
Polyclonal
特異性:
SLC22A6 Antibody detects endogenous levels of total SLC22A6.
RRID:
AB_2841786
引用形式: Affinity Biosciences Cat# DF8582, RRID:AB_2841786.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

FLJ55736; hOAT1; hPAHT; hROAT1; MGC45260; OAT1; Organic anion transporter 1; OTTHUMP00000236796; OTTHUMP00000236797; OTTHUMP00000236798; OTTHUMP00000236799; PAH transporter; PAHT; Para aminohippurate transporter; Renal organic anion transporter 1; ROAT1; S22A6_HUMAN; SLC22A6; Solute carrier family 22 (organic anion transporter) member 6; Solute carrier family 22 member 6;

免疫原

免疫原:
Uniprot:

Q4U2R8(S22A6_HUMAN) >>Visit HPA database.

遺伝子(ID):
SLC22A6(9356)
発現特異性:
Q4U2R8 S22A6_HUMAN:

Strongly expressed in kidney and to a lower extent in liver, skeletal muscle, brain and placenta. Found at the basolateral membrane of the proximal tubule.

タンパク質配列:
MAFNDLLQQVGGVGRFQQIQVTLVVLPLLLMASHNTLQNFTAAIPTHHCRPPADANLSKNGGLEVWLPRDRQGQPESCLRFTSPQWGLPFLNGTEANGTGATEPCTDGWIYDNSTFPSTIVTEWDLVCSHRALRQLAQSLYMVGVLLGAMVFGYLADRLGRRKVLILNYLQTAVSGTCAAFAPNFPIYCAFRLLSGMALAGISLNCMTLNVEWMPIHTRACVGTLIGYVYSLGQFLLAGVAYAVPHWRHLQLLVSAPFFAFFIYSWFFIESARWHSSSGRLDLTLRALQRVARINGKREEGAKLSMEVLRASLQKELTMGKGQASAMELLRCPTLRHLFLCLSMLWFATSFAYYGLVMDLQGFGVSIYLIQVIFGAVDLPAKLVGFLVINSLGRRPAQMAALLLAGICILLNGVIPQDQSIVRTSLAVLGKGCLAASFNCIFLYTGELYPTMIRQTGMGMGSTMARVGSIVSPLVSMTAELYPSMPLFIYGAVPVAASAVTVLLPETLGQPLPDTVQDLESRWAPTQKEAGIYPRKGKQTRQQQEHQKYMVPLQASAQEKNGL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
100
Horse
88
Sheep
88
Dog
88
Bovine
75
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q4U2R8 基板として

Site PTM Type Enzyme
N39 N-Glycosylation
N56 N-Glycosylation
N92 N-Glycosylation
N97 N-Glycosylation
N113 N-Glycosylation
K297 Ubiquitination
K303 Ubiquitination
K315 Ubiquitination
T318 Phosphorylation
S325 Phosphorylation

研究背景

機能:

Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of 2,3-dimercapto-1-propanesulfonic acid (DMPS) (By similarity). Mediates the sodium-independent uptake of p-aminohippurate (PAH), ochratoxin (OTA), acyclovir (ACV), 3'-azido-3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF), cidofovir, adefovir, 9-(2-phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl) diaminopurine (PMEDAP) and edaravone sulfate. PAH uptake is inhibited by p-chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), sulindac, diclofenac, carprofen, glutarate and okadaic acid (By similarity). PAH uptake is inhibited by benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, furosemide, indomethacin, bumetamide, losartan, probenecid, phenol red, urate, and alpha-ketoglutarate.

PTMs:

Glycosylated. Glycosylation at Asn-113 may occur at a secondary level. Glycosylation is necessary for proper targeting of the transporter to the plasma membrane.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Strongly expressed in kidney and to a lower extent in liver, skeletal muscle, brain and placenta. Found at the basolateral membrane of the proximal tubule.

タンパク質ファミリー:

Multiple cysteine residues are necessary for proper targeting to the plasma membrane.

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.

参考文献

1). Hypouricemic effect of gallic acid, a bioactive compound from Sonneratia apetala leaves and branches, on hyperuricemic mice. Food & Function, 2022 (PubMed: 36125096) [IF=6.1]
2). Ameliorative action of "daitongxiao" against hyperuricemia includes the "uric acid transporter group". Frontiers in pharmacology, 2024 (PubMed: 38983915) [IF=5.6]

Application: WB    Species: quail    Sample: kidneys

FIGURE 4 DTX treatment decreased the expression of GLUT9, OAT1, and OAT3 in the HUA quail kidneys (n = 3). The melting curves of RT-PCR products of renal (A) GLUT9, (B) OAT3, (C) GAPDH, (D) OAT1, and (E) β-actin in the quail kidneys. The amplification curve of the RT-PCR products of (F) GLUT9, (G) OAT3, (H) GAPDH, (I) OAT1, and (J) β-actin in the quail kidneys. The bar diagram showing the relative expression levels of (K) GLUT9 mRNA, (L) OAT3 mRNA, and (M) OAT1 mRNA. (N) Results of Western blotting showing the relative expression levels of GLUT9, OAT1, OAT3, and GAPDH in the kidneys of control, HUA model, and drug-treated quails. An equal amount (75 µg/lane) of total protein was loaded in each lane of 10% SDS-polyacrylamide gel, fractionated, and then analyzed. The bar diagram shows the relative expression levels of the (O) GLUT9 protein, (P) OAT1 protein, and (Q) OAT3 protein in the quail kidneys. Relative expression was estimated after normalizing with GAPDH/β-actin. #p < 0.05 and ##p < 0.01 compared with the control group. *p < 0.05 and **p < 0.01 compared with the model group; ns: p > 0.05.

Application: IF/ICC    Species: Rat    Sample: kidneys

FIGURE 6 DTX decreased the expression of GLUT9 and increased the expression of OAT1 and OAT3 in rat kidneys (n = 3). The melting curve of the RT-PCR product of renal (A) GLUT9, (B) OAT1, (C) OAT3, and (D) GAPDH of rats. The amplification curve of the RT-PCR product of renal (E) GLUT9, (F) OAT1, (G) OAT3, and (H) GAPDH of rats. The representative photomicrographs showing the immunodetection of (I) GLUT9, (J) OAT1, and (K) OAT3 protein expression in rat kidney sections. The bar diagrams show the relative expression levels of renal (L) GLUT9 mRNA, (M) OAT1 mRNA, (N) OAT3 mRNA, (O) GLUT9 protein, (P) OAT1 protein, and (Q) OAT3 protein in control, HUA, and DTX-treated HUA rats. The mean fluorescent intensity was measured across the selected region of each subfigure of immunofluorescence. Compared with the control group, #p < 0.05 and ##p < 0.01. Compared with the model group, *p < 0.05 and **p < 0.01.
3). Hypouricaemic and nephroprotective effects of Poria cocos in hyperuricemic mice by up-regulating ATP-binding cassette super-family G member 2. PHARMACEUTICAL BIOLOGY, 2021 (PubMed: 33651969) [IF=3.8]

Application: WB    Species: Mice    Sample: kidney tissues

Figure 4. Effects of PCE and PCW on renal ABCG2, OAT3, OAT1 and OCT2 protein expression detected by Western blot: immunoreactive bands (a) and densitometries (b,c,d and –e, expressed as mean ± SD; n n ¼ 3).  p < 0.05,  p < 0.01 versus the normal control; #p < 0.05, ##p < 0.01 versus the hyperuricemic control;  p < 0.01 versus the allopurinol control.
4). Ethanol extract of Nepeta angustifoia C. Y. Wu ameliorates hyperuricemia in fructose-induced mice. Pakistan journal of pharmaceutical sciences, 2022 (PubMed: 36008897) [IF=0.8]

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